Summary
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1.
The immersion fixation effect of aceton, ethanol, formaldehyde, hydroxyadipaldehyde, glutaraldehyde on the CAH activity of rat liver and kidney cortex has been examined.
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2.
The activity is observed to be preserved best after aceton and hydroxyadipaldehyde fixation.
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3.
The histochemical reaction is most successful, when hydroxyadipaldehyde is used. The other aldehydes are best suited for fixing big blocks.
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4.
The localization of CAH activity is found to be in the luminal surface of the kidney tubule cells, which gives support to the mechanism of urinary acidification presented in literature. Differences in localization with different aldehyde fixations are not observed.
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Hyyppä, M. The effects of fixations on Carbonic anhydrase activity. Histochemie 12, 184–188 (1967). https://doi.org/10.1007/BF00310369
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DOI: https://doi.org/10.1007/BF00310369