Skip to main content
SpringerLink
Log in

Expression of the wheat chloroplast gene for CF0 subunit IV of ATP synthase

  • Original Articles
  • Published:
Current Genetics Aims and scope Submit manuscript

Summary

The nucleotide sequence of the wheat chloroplast atp I gene encoding CF0 subunit IV of ATP synthase has been determined. The gene encodes a polypeptide of 247 amino acid residues with high sequence similarity to subunit IV from other plant chloroplasts and from cyanobacteria. The polypeptide shows sequence homology to the C-terminus of the F0 a subunit of Escherichia coli ATP synthase and subunit 6 of mitochondrial ATP synthase. The atp I gene is co-transcribed with the atp H, atp F and atp A genes for other subunits of ATP synthase in wheat. A gene-fusion of most of the atpI coding region with cro 1-lacI'-lacZ' has been constructed in pEX2 and the fusion-protein has been used to raise antibodies in rabbits. The antibodies react with a polypeptide of 17 kDa in wheat thylakoid membranes indicating that the wheat atpI gene is expressed at the protein level. A model for the organisation of the polypeptide in the thylakoid membrane with four membrane-spanning segments is proposed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anderson S, Bankier AT, Barrell BG, de Bruijn MHL, Coulson AR, Drouin J, Eperon I, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG (1981) Nature 290: 457–465

    Google Scholar 

  • Anderson S, de Bruijn MHL, Coulson AR, Eperon IC, Sanger F, Young IG (1982) J Mol Biol 156: 683–717

    Google Scholar 

  • Berthold DA, Babcock GT, Yocum CF (1981) FEBS Lett 134: 231–234

    Google Scholar 

  • Berzborn RJ, Otto J, Finke W, Meyer HE, Block J (1987) Biol Chem Hoppe-Seyler 368: 551–552

    Google Scholar 

  • Bibb MJ, Van Etten RA, Wright CT, Walberg MW, Clayton DA (1981) Cell 26: 167–180

    Google Scholar 

  • Bird CR, Koller B, Auffret AD, Huttly AK, Howe CJ, Dyer TA, Gray JC (1985) EMBO J 4: 1381–1388

    Google Scholar 

  • Bland MM, Levings CS, Matzinger DF (1987) Curr Genet 12: 475–487

    Google Scholar 

  • Bowman CM, Koller B, Delius H, Dyer TA (1981) Mol Gen Genet 183: 93–101

    Google Scholar 

  • Breen GA, Miller DL, Holmans PL, Welch G (1985) J Biol Chem 261: 11680–11685

    Google Scholar 

  • Cain BD, Simoni RD (1986) J Biol Chem 261: 10043–10050

    Google Scholar 

  • Cain BD, Simoni RD (1988) J Biol Chem 263: 6606–6612

    Google Scholar 

  • Christophe D, Brocas H, Vassart G (1982) Anal Biochem 120: 259–261

    Google Scholar 

  • Clary DO, Wolstenhome DR (1985) J Mol Evol 22: 252–271

    Google Scholar 

  • Covey SN, Hull R (1981) Virology 111: 463–474

    Google Scholar 

  • Cozens AL, Walker JE (1987) J Mol Biol 194: 359–383

    Google Scholar 

  • Cozens AL, Walker JE, Phillips AL, Huttly AK, Gray JC (1986) EMBO J 5: 217–222

    Google Scholar 

  • Cox GB, Fimmel AL, Gibson F, Hatch L (1986) Biochim Biophys Acta 849: 62–69

    Google Scholar 

  • Curtis SE (1988) Photosynth Res 18: 223–244

    Google Scholar 

  • Dale RMK, McClure BA, Houchins JP (1985) Plasmid 13: 31–40

    Google Scholar 

  • Dewey RE, Levings CS, Timothy DH (1985) Plant Physiol 79: 914–919

    Google Scholar 

  • Filligame RH (1981) Curr Top Bioenerget 11: 31–106

    Google Scholar 

  • Fromme P, Graber P, Salnikow J (1987) FEBS Lett 218: 27–30

    Google Scholar 

  • Gray JC, Dunn PPJ, Eccles CJ, Green RM, Hird SM, Höglund AS, Webber AN, Willey DL, Dyer TA (1988) Biochem Soc Trans 16: 704–706

    Google Scholar 

  • Grisi E, Brown TA, Waring RB, Scazzochio C, Davies RW (1982) Nucleic Acids Res 10: 3531–3539

    Google Scholar 

  • Grosskopf R, Feldmann H (1981) Curr Genet 4: 151–158

    Google Scholar 

  • Gunsalus RP, Brusilow WSA, Simoni RD (1982) Proc Natl Acad Sci USA 79: 320–324

    Google Scholar 

  • Henikoff S (1984) Gene 28: 351–359

    Google Scholar 

  • Hennig J, Herrmann RG (1986) Mol Gen Genet 203: 117–128

    Google Scholar 

  • Höglund AS, Gray JC (1987) Nucleic Acids Res 15: 10590

    Google Scholar 

  • Howe CJ, Auffret A, Doherty A, Bowman CM, Dyer TA, Gray JC (1982) Proc Natl Acad Sci USA 79: 6903–6907

    Google Scholar 

  • Hudson GS, Mason JG, Holton TA, Koller B, Cox GB, Whitfeld PR, Bottomley W (1987) J Mol Biol 196: 283–298

    Google Scholar 

  • Huttly AK, Gray JC (1984) Mol Gen Genet 194: 402–409

    Google Scholar 

  • John UP, Wilson TA, Linnane AW, Nagley P (1986) Nucleic Acids Res 14: 7437–7451

    Google Scholar 

  • Kumamoto CA, Simoni RD (1986) J Biol Chem 261: 10037–10042

    Google Scholar 

  • Laemmli UK (1970) Nature 227: 680–685

    Google Scholar 

  • Lightowlers RN, Howitt SM, Hatch L, Gibson F, Cox GB (1987) Biochim Biophys Acta 894: 399–406

    Google Scholar 

  • Macino G, Tzagoloff A (1980) Cell 20: 507–517

    Google Scholar 

  • Morelli G, Macino G (1984) J Mol Biol 178: 491–507

    Google Scholar 

  • Nelson N (1981) Curr Top Bioenerget 11: 1–33

    Google Scholar 

  • Newman BJ, Gray JC (1988) Plant Mol Biol 10: 511–520

    Google Scholar 

  • Ohta S, Yohda M, Ishizuka M, Hirata H, Hamamoto T, Otawara-Hamamoto Y, Matsuda K, Kagawa Y (1988) Biochim Biophys Acta 933: 141–155

    Google Scholar 

  • Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Nature 322: 572–574

    Google Scholar 

  • Pick U, Racker E (1979) J Biol Chem 254: 2793–2799

    Google Scholar 

  • Plant AL, Covey SN, Grierson D (1985) Nucleic Acids Res 13: 8305–8321

    Google Scholar 

  • Roe BA, Din-Pow M, Wilson RK, Wong JFH (1985) J Biol Chem 260: 9759–9774

    Google Scholar 

  • Sanger F, Coulson AR, Barrell BG, Smith AJH, Roe BA (1980) J Mol Biol 143: 161–178

    Google Scholar 

  • Schneider E, Altendorf K (1985) EMBO J 4: 515–518

    Google Scholar 

  • Schuster W, Brennicke A (1987) Nucleic Acids Res 15: 9092

    Google Scholar 

  • Sebald W, Wachter E (1980) FEBS Lett 122: 307–311

    Google Scholar 

  • Senior AE (1983) Biochim Biophys Acta 726: 81–95

    Google Scholar 

  • Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohta C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5: 2043–2049

    Google Scholar 

  • Stanley KK, Luzio JP (1984) EMBO J 3: 1429–1434

    Google Scholar 

  • Süss KH (1980) FEBS Lett 112: 255–259

    Google Scholar 

  • Vik SB, Cain BD, Chun KT, Simoni RD (1988) J Biol Chem 263: 6599–6605

    Google Scholar 

  • Von Heijne G (1983) Eur J Biochem 133: 17–21

    Google Scholar 

  • Von Meyenberg K, Jorgensen BB, Michelsen O, Sorensen L, McCarthy JEG (1985) EMBO J 4: 2357–2363

    Google Scholar 

  • Walker JE, Saraste M, Gay NJ (1984) Biochim Biophys Acta 768: 164–200

    Google Scholar 

  • Webber AN, Hird SM, Packman LC, Dyer TA, Gray JC (1989a) Plant Mol Biol 12: 141–151

    Google Scholar 

  • Webber AN, Packman LC, Chapman DJ, Barber J, Gray JC (1989b) FEBS Lett 242: 259–262

    Google Scholar 

  • Westhoff P, Alt J, Nelson N, Herrmann RG (1985) Mol Gen Genet 199: 290–299

    Google Scholar 

  • Yen TSB, Webster RE (1981) J Biol Chem 256: 11259–11265

    Google Scholar 

Download references

Author information

Author notes

  1. Anna-Stina Höglund

    Present address: Department of Cell Research, Uppsala Biomedical Center, University of Agricultural Sciences, Box 596, S-75124, Uppsala, Sweden

  2. Aine L. Plant

    Present address: Department of Botany and Plant Science, University of California, 92521, Riverside, CA, USA

Authors and Affiliations

  1. Botany School, University of Cambridge, Downing Street, CB2 3EA, Cambridge, England, UK

    Anna-Stina Höglund, Aine L. Plant & John C. Gray

Authors
  1. Anna-Stina Höglund
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Aine L. Plant
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. John C. Gray
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by C. J. Leaver

Rights and permissions

Reprints and permissions

About this article

Cite this article

Höglund, AS., Plant, A.L. & Gray, J.C. Expression of the wheat chloroplast gene for CF0 subunit IV of ATP synthase. Curr Genet 18, 471–476 (1990). https://doi.org/10.1007/BF00309919

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00309919

Key words

Search

Navigation