Summary
The distribution of gamma-aminobutyric acid (GABA)-like and glutamate decarboxylase (GAD)-like immunoreactivity was studied in the cervical sympathetic ganglion complex of rats, including the intermediate and inferior cervical ganglia and the uppermost thoracic ganglion. GABA-positive axons may enter the ganglion complex via its caudal end. Others apparently arise from small GABA-positive cell bodies which are scattered among principal neurons, within clusters of SIF cells and in bundles of GABA-negative axons. The majority of these cells is located in the lower half of the ganglion complex. Principal neurons did not react with antibodies against GABA or GAD. An unevenly distributed meshwork of GABA-immunoreactive axons was seen in each of the ganglia. Immunoreactive axons formed numerous varicosities. Some of them were aggregated in a basket-like form around a subpopulation of GABA-negative principal ganglion cell bodies. Electron-microscopic immunocytochemistry revealed that GABA-positive nerve fibers establish asymmetric synaptic junctions with dendritic and somatic spines of principal neurons, whereas postsynaptic densities are inconspicous or absent on dendritic shafts and somata. The results suggest that in the cervical sympathetic ganglion complex principal neurons are not GABAergic, but are innervated by axons which react with both antibodies against GAD and/ or GABA antibodies and originate from a subpopulation of small neurons.
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References
Appenzeller O (1982) The autonomic nervous system. Elsevier, Amsterdam
Arbab MA-R, Wiklund L, Delgado T, Svengaard NA (1988) Stellate ganglion innervation of the vertebrobasilar arterial system demonstrated in the rat with anterograde and retrograde WGA-HRP tracing. Brain Res 445:175–180
Armour JA, Hopkins DA (1981) Localization of sympathetic postganglionic neurons of physiologically identified cardiac nerves in the dog. J Comp Neurol 202:169–184
Bachoo M, Isacoff E, Polosa C (1986) Properties of noncholinergic mechanisms in cat stellate ganglion (SG) for control of heart rate (HR). Proc Int Union Physiol Sci 16:318–321
Bacon SJ, Smith AD (1988) Preganglionic sympathetic neurones innervating the rat adrenal medulla: immunocytochemical evidence of synaptic input from nerve terminals containing substance P, GABA or 5-hydroxytryptamine. J Auton Nerv Syst 24:97–122
Barber RP, Vaughn JE, Roberts E (1982) The cytoarchitecture of GABAergic neurones in the rat spinal cord. Brain Res 238:305–328
Bosnjak ZJ, Kampine JP (1985) Electrophysiological and morphological characterization of neurons in stellate ganglion of cats. Am J Physiol 248, Regul Integrative Comp Physiol 17:R288-R292
Bowers CW, Zigmond RE (1981) Sympathetic neurons in lower cervical ganglia send axons through the superior cervical ganglion. Neuroscience 6:1783–1791
Bowery NG (1986) Introduction. In: Erdö SL, Bowery NG (eds) GABAergic Mechanisms in the Mammalian Periphery. Raven Press, New York, pp 1–3
Dail WG, Barton S (1983) Structure and organization of mammalian sympathetic ganglia. In: Elfvin L-G (ed) Autonomic ganglia. Wiley, New York, pp 3–25
Darvesh S, Nance DM, Hopkins DA, Armour JA (1987) Distribution of neuropeptide-like immunoreactivity in intact and chronically decentralized middle cervical and stellate ganglia of dogs. J Auton Nerv Syst 21:167–180
Davanger S, Ottersen OP, Storm-Mathisen J (1987) Immunocytochemical localization of GABA in cat myenteric plexus. Neurosci Lett 73:27–32
Dobó E, Kása P, Wenthold RJ, Wolff JR (1989a) Pronase treatment increases the staining intensity of GABA-immunoreactive structures in the paravertebral sympathetic ganglia. Histochemistry 93:13–18
Dobó E, Kása P, Wenthold RJ, Joó F, Wolff JR (1989b) Evidence for GABAergic fibers entering the superior cervical ganglion of rat from the preganglionic nerve trunk. Histochemistry 92:133–136
Elfvin L-G (1971) Ultrastructural studies on the synaptology of the inferior mesenteric ganglion of the cat. I Observation on the cell surface of the postganglionic perikarya. J Ultrastruct Res 37:411–425
Elfvin L-G (1983) Autonomic ganglia. Wiley, New York
Fränkö O, Härkönen M (1965) Monoamine-containing cells in the superior cervical ganglion of the rat and organ composed of them Acta Physiol Scand 63:511–512
Erdö SL, Bowery NG (1986) GABAergic mechanisms in the mammalian periphery. Raven Press, New York
Gabella G (1976) Structure of the autonomic nervous system. Chapman and Hall, London
Gordon-Weeks PR (1988) The ultrastructure of noradrenergic and cholinergic neurons in the autonomic nervous system. In: Björklund A, Hökfelt T, Guzman C (eds) Handbook of chemical neuroanatomy. Elsevier, Amsterdam New York Oxford, pp 117–142
Greene EC (1968) Anatomy of the rat. Hafner, New York, pp 134–135
Häppölä O, Päivärinta H, Soinila S, Wu J-Y, Panula P (1987) Localization of L-glutamate decarboxylase and GABA transaminase immunoreactivity in the sympathetic ganglia of the rat. Neuroscience 21:271–281
Hedger JH, Webber RH (1976) Anatomical study of the cervical sympathetic trunk and ganglia in the albino rat (Mus norvegicus albinus). Acta Anat 96:206–217
Heym Ch, Reinecke M, Weihe E, Forssmann WG (1984) Dopamine-beta-hydroxylase-, neurotensin-, substance P-, vasoactive intestinal polypeptide- and enkephalin-immunohistochemistry of paravertebral and prevertebral ganglia in the cat. Cell Tissue Res 235:411–418
Hills JM, Jessen KR, Mirsky R (1987) An immunohistochemical study of the distribution of enteric GABA-containing neurons in the rat and guinea pig intestine. Neuroscience 22:301–312
Jessen KR, Hills JM, Saffrey M (1986) Immunohistochemical demonstration of GABAergic neurons in the enteric nervous system. J Neurosci 6:1628–1634
Kása P, Joó E, Dobó E, Wenthold RJ, Ottersen OP, Storm-Mathisen J, Wolff JR (1988) Heterogeneous distribution of GABA-immunoreactive nerve fibers and axon terminals in the superior cervical ganglion of adult rat. Neuroscience 26:635–644
Kenny SL, Ariano MA (1986) The immunofluorescence localization of glutamate decarboxylase in the rat superior cervical ganglion. J Auton Nery Syst 17:211–215
Kharkevich DA (1980) Pharmacology of ganglionic transmission. Handbook of experimental pharmacology. Springer, Berlin Heidelberg New York
Kiernan JA (1981) Histological and histochemical methods: theory and practice, 2nd edn. Pergamon Press, Oxford, p 29
Kosaka T, Tauchi M, Dahl JL (1988) Cholinergic neurons containing GABA-like and/or glutamic acid decarboxylase-like immunoreactivities in various brain regions of the rat. Exp Brain Res 70:605–617
Lindh B, Staines W, Hökfelt T, Terenius L, Salvaterra PM (1986) Immunohistochemical demonstration of choline acetyltransferase-immunoreactive preganglionic nerve fibers in guinea-pig autonomic ganglia. Proc Natl Acad Sci USA 83:5316–5320
Matthews MR (1983) The ultrastructure of junctions in sympathetic ganglia of mammals. In: Elfvin L-G (ed) Autonomic ganglia. Wiley, New York, pp 27–66
Oertel WH, Schmechel DE, Tappaz ML, Kopin IJ (1981) Production of a specific antiserum to rat brain glutamic acid decarboxylase by injection of an antigen-antibody complex. Neuroscience 6:2689–2700
Ottersen OP, Storm-Mathisen J, Madsen S, Skumlien S, Stromhaug J (1986) Evaluation of the immunocytochemical method for amino acids. Med Biol 64:147–158
Pelto-Huikko M, Heryonen A, Helen P, Linnoila I, Pickel VM, Miller RJ (1980) Localization of (Met5)-and (Leu5)-enkephalin in nerve terminals and SIF cells in adult human sympathetic ganglia. In: Eränkö O, Soinila S, Päivärinta H (eds) Histochemistry and cell biology of autonomic neurons, SIF cells, and paraneurons. Raven Press, New York, pp 379–383
Saito N, Tanaka C (1986) Immunohistochemical demonstration of GABA containing neurons in the guinea pig ileum using purified GABA antiserum. Brain Res 376:78–84
Schultzberg M, Lindh B (1988) Transmitters and peptides in autonomic ganglia. In: Björklund A, Hökfelt T, Owman C (eds) Handbook of chemical neuroanatomy, Vol 6. Elsevier, Amsterdam New York Oxford, pp 297–326
Verhofstad AAJ, Steinbusch HWM, Penke B, Varga J, Joosten HWM (1981) Serotonin-immunoreactive cells in the superior cervical ganglion of the rat. Evidence for the existence of separate serotonin- and catecholamine-containing small ganglion cells. Brain Res 212:39–49
Wenthold RJ, Zempel JM, Parakkal MH, Reeks KA, Altschuler RA (1986) Immunocytochemical localization of GABA in the cochlear nucleus of the guinea pig. Brain Res 380:7–18
Wolff JR (1981) Evidence for dual role of GABA as a synaptic transmitter and a promoter of synaptogenesis. In: DeFeudis FN, Mandel P (eds) Amino Acids Neurotransmitters. Raven Press, New York, pp 459–466
Wolff JR, Joó F, Kása P, Storm-Mathisen J, Toldi J, Balcar V (1986) Presence of neurons with GABA-like immunoreactivity in the superior cervical ganglion of the rat. Neurosci Lett 71:157–162
Wolff JR, Joó F, Kása P (1987) Synaptic, metabolic and morphogenetic effects of GABA in the superior cervical ganglion of rats. In: Redburn D, Schousboe A (eds) Neurotrophic activity of GABA during development. Liss, New York, pp 221–252
Wolff JR, Kása P, Dobó E, Joó F (1988) GABAergic innervation of sympathetic ganglion cells. In: Elsner N, Creutzfeldt O (eds) New frontiers in brain research. Thieme, Stuttgart, p 313
Wolff JR, Kása P, Dobó E, Wenthold RJ, Joó F (1989) Quantitative analysis of the number and distribution of neurons richly innervated by GABA-immunoreactive axons in the rat superior cervical ganglion. J Comp Neurol 282:264–273
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Dobó, E., Kása, P., Joó, F. et al. Structures with GABA-like and GAD-like immunoreactivity in the cervical sympathetic ganglion complex of adult rats. Cell Tissue Res 262, 351–361 (1990). https://doi.org/10.1007/BF00309890
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DOI: https://doi.org/10.1007/BF00309890