Skip to main content
SpringerLink
Log in

Fibronectin distribution during cell type conversion in newt lens regeneration

  • Original Articles
  • Published:
Anatomy and Embryology Aims and scope Submit manuscript

Summary

The distribution of fibronectin during the cell type conversion from iris into lens that occurs in newt lens regeneration was studied by immunofluorescence. Newts were lentectomized and irises at different stages of dedifferentiation and redifferentiation were examined using as a probe a rabbit antiserum prepared to Xenopus plasma fibronectin. In the normal iris, fibronectin is predominantly located at the basal surface of the pigmented iris epithelial cells. During activation and early dedifferentiation fibronectin staining is progressively displayed at the basolateral and apical surface of the depigmenting cell, to eventually surround the surface of the dedifferentiated cells. As cells redifferentiate into lens fibers, staining for cell surface fibronectin decreases and is displayed mainly in the nascent lens capsule. Fibronectin deposition may be associated with the formation of intercellular spaces during dedifferentiation. The fibronectin-rich extracellular matrix could be important in cell reprogramming.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ali IU, Hynes RO (1978) Effects of LETS glycoprotein on cell motility. Cell 14:439–446

    Google Scholar 

  • Balian G, Click EM, Crouch E, Davidson JM, Borstein P (1979) Isolation of a collagen-binding fragment from fibronectin and cold-insoluble globulin. J Biol Chem 254:1429–1432

    Google Scholar 

  • Boucaut JC, Darribere T (1983) Fibronectin in early amphibian embryos: migrating mesodermal cells contact fibronectin established prior to gastrulation. Cell Tissue Res 234:135–145

    Google Scholar 

  • Boucaut JC, Darribere T, Boulekbache H, Thiery JP (1984) Prevention of gastrulation but not neurulation by antibodies to fibronectin in amphibian embryos. Nature 307:364–366

    Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Google Scholar 

  • Chen JM, Chen WT (1987) Fibronectin-degrading proteases from the membranes of transformed cells. Cell 48:193–203

    Google Scholar 

  • Chen W, Hasegawa E, Hasegawa T, Weinstock C, Yamada KM (1985) Development of cell surface linkage complexes in cultured fibroblasts. J Cell Biol 100:1103–1114

    Google Scholar 

  • Crichley DR, England MA, Wakely J, Hynes RO (1979) Distribution of fibronectin in the ectoderm of gastrulating chick embryos. Nature 280:498–500

    Google Scholar 

  • Darribere T, Boucher D, Lacroix JC, Boucaut JC (1984) Fibronectin synthesis during oogenesis and early development of the amphibian Pleurodeles waltlii. Cell Differ 14:171–177

    Google Scholar 

  • Dessau W, Jilek F, Adelmann BC, Hormann H (1978) Similarity of antigelatin factor and cold insoluble globulin. Biochim Biophys Acta 533:227–237

    Google Scholar 

  • Dumont JN, Yamada T (1972) Dedifferentiation of iris epithelial cells. Dev Biol 29:385–401

    Google Scholar 

  • Dumont JN, Yamada T, Cone VM (1970) Alteration of nucleolar ultrastructure in iris epithelial cells during initiation of Wolffian lens regeneration. J Exp Zool 174:187–204

    Google Scholar 

  • Eisenberg S, Yamada T (1966) A study of DNA synthesis during the transformation of the iris into lens in the lentectomized newt. J Exp Zool 162:353–368

    Google Scholar 

  • Elgert KL, Zalik SE (1986) Fibronectin distribution during newt lens regeneration. J Cell Biol 103:251a

    Google Scholar 

  • Greenberg JH, Seppa S, Seppa H, Hewitt AT (1981) Role of collagen and fibronectin in neural crest cell adhesion and migration. Dev Biol 87:259–266

    Google Scholar 

  • Gulati AK, Zalewski AA, Reddi AH (1983) An immunofluorescent study of the distribution of fibronectin and laminin during limb regeneration in the adult newt. Dev Biol 96:355–365

    Google Scholar 

  • Hawkes R, Niday E, Gordon J (1982) A dot immunobinding assay for monoclonal and other antibodies. Anal Biochem 119:142–147

    Google Scholar 

  • Hayashi M, Yamada K (1981) Differences in domain structures between plasma and cellular fibronectins. J Biol Chem 256:11292–11300

    Google Scholar 

  • Hayashi M, Yamada KM (1982) Divalent cation modulation of fibronectin binding to heparin and to DNA. J Biol Chem 257:5263–5267

    Google Scholar 

  • Hayashi M, Yamada KM (1983) Domain structure of the carboxylterminal half of human plasma fibronectin. J Biol Chem 258:3332–3340

    Google Scholar 

  • Heasman J, Hynes RO, Swan AP, Thomas V, Wylie CC (1981) Primordial germ cells of Xenopus embryos: the role of fibronectin in their adhesion during migration. Cell 27:437–447

    Google Scholar 

  • Hynes RO (1982) Fibronectin and its relation to cellular structure and behavior. In: Hay ED (ed) Cell Biology of Extracellular Matrix. Plenum Press, New York, pp 295–334

    Google Scholar 

  • Jilek F, Hormann H (1977) Cold-insoluble globulin, II. Plasminolysis of cold-insoluble globulin. Hoppe-Seyler's Zeits. Physiol Chem 358:133–136

    Google Scholar 

  • Johnson GD, Davidson RS, McNamee KC, Goodwin RD, Holborow EJ (1982) Fading in immunofluorescence during microscopy: a study of the phenomenon and its remedy. J Immunol Methods 55:231–242

    Google Scholar 

  • Karasaki S (1964) An electron microscopic study of Wolffian lens regeneration in the adult newt. J Ultrastruct Res 11:246–273

    Google Scholar 

  • Keski-Oja J, Yamada K (1981) Isolation of an actin-binding fragment of fibronectin. Biochem J 193:615–620

    Google Scholar 

  • Kulyk WM, Zalik SE (1982) Synthesis of sulfated glycosaminoglycans in newt iris during lens regeneration. Differentiation 23:29–35

    Google Scholar 

  • Kulyk WM, Zalik SE, Dimitrov E (1987) Hyaluronic acid production and hyaluronidase activity in the newt iris during lens regeneration. Exp Cell Res 172:180–191

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Google Scholar 

  • Lee G, Hynes RO, Kirschner M (1984) Temporal and spatial regulation of fibronectin in early Xenopus development. Cell 36:729–740

    Google Scholar 

  • McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative: a new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1084

    Google Scholar 

  • Obrink B (1982) Hepatocyte — collagen adhesion. In: Cunnigham LW, Fredericksen (eds) Methods in Enzymology, vol 82, Part A. Academic Press, NY, pp 513–529

    Google Scholar 

  • Patmore M, Yamada T (1982) The role of calcium in depigmentation of iris epithelial cells during cell-type conversion. Dev Biol 92:266–274

    Google Scholar 

  • Pearlstein E (1976) Plasma membrane glycoprotein which mediates adhesion of fibroblasts to collagen. Nature 262:497–499

    Google Scholar 

  • Reese DH (1973) In vitro initiation in the newt iris of some early molecular events of lens regeneration. Exp Eye Res 17:435–444

    Google Scholar 

  • Reese DH, Puccia E, Yamada T (1969) Activation of ribosomal RNA synthesis in initiation of Wolffian lens regneration. J Exp Zool 170:259–268

    Google Scholar 

  • Repesh LA, Furcht LT, Smith D (1981) Immunocytochemical localization of fibronectin in limb tissues of the adult newt Notophthalmus viridescens. J Histochem Cytochem 29:937–945

    Google Scholar 

  • Repesh LA, Fitzgerald TJ, Furcht LT (1982) Changes in the distribution of fibronectin during limb regeneration in newts using immunocytochemistry. Differentiation 22:125–131

    Google Scholar 

  • Reyer RW (1977) The amphibian eye: development and regeneration. In: Crescitelli F (ed) Handbook of Sensory Physiology, vol 7 (5). Springer Verlag, Berlin Heidelberg, pp 309–390

    Google Scholar 

  • Ruoslahti E, Hayman EG, Kuusela P, Shively JE, Engvall E (1979) Isolation of tryptic fragments containing the collagen-binding site of plasma fibronectin. J Biol Chem 254:6054–6059

    Google Scholar 

  • Ruoslahti E, Hayman EG, Pierschbacher M, Engvall E (1982) Fibronectin: purification, immunochemical properties and biological activities. In: Cunningham LW, Frederiksen DW (eds) Methods in Enzymology, vol 82, Part A. Academic Press, New York, pp 803–830

    Google Scholar 

  • Saba TM, Jaffe E (1980) Plasma fibronectin (opsonic glycoprotein): Its synthesis by vascular endothelial cells and role in cardiopulmonary integrity after trauma as related to reticuloendothelial function. Am J Med 68:577–594

    Google Scholar 

  • Sekiguchi K, Hakomori S (1980) Functional domain structure of fibronectin. Proc Natl Acad Sci USA 77:2661–2665

    Google Scholar 

  • Stone LS (1967) An investigation recording all salamanders which can and cannot regenerate a lens from the dorsal iris. J Exp Zool 164:87–104

    Google Scholar 

  • Sugrue SP, Hay ED (1981) Response of basal epithelial cell surface and cytoskeleton to solubilized extracellular matrix molecules. J Cell Biol 19:45–54

    Google Scholar 

  • Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354

    Google Scholar 

  • Van der Water III L, Schroeder S, Crenshaw III BE, Hynes RO (1981) Phagocytosis of gelatin-latex particles by a murine macrophage line is dependent on fibronectin and heparin. J Cell Biol 90:32–39

    Google Scholar 

  • Yamada T (1977) Control mechanisms in cell-type conversion in newt lens regeneration. In: Wolsky A (ed) Monographis in Development Biology, vol 13, S Karger, Basel

    Google Scholar 

  • Yamada KM (1981) Fibronectin and other structural proteins. In: Hay ED (ed) Cell Biology of Extracellular Matrix. Plenum Press, New York, pp 95–114

    Google Scholar 

  • Yamada KM, Kennedy DW (1984) Dualistic nature of adhesive protein function: Fibronectin and its biologically active peptide fragments can autoinhibit fibronectin function. J Cell Biol 99:29–26

    Google Scholar 

  • Yamada T, McDevitt DS (1984) Conversion of iris epithelial cells as a model of differentiation control. Differentiation 27:1–12

    Google Scholar 

  • Yamada KM, Olden K, Pastan I (1978) Transformation-sensitive cell surface protein: Isolation, characterization and role in cellular morphology and adhesion. Ann NY Acad Sci 312:256–277

    Google Scholar 

  • Yamada T, Roesel ME (1969) Activation of DNA replication in the iris epithelium by lens removal. J Exp Zool 171:425–432

    Google Scholar 

  • Yamada T, Takata C (1963) An autoradiographic study of protein synthesis in regenerative tissue transformation of iris into lens in the newt. Dev Biol 8:358–369

    Google Scholar 

  • Yamada KM, Humphries MJ, Hasegawa T, Hasegawa E, Olden K, Chen WT, Akiyama ST (1985) Fibronectin: Molecular approaches to analyzing cell interactions with the extracellular matrix. In: Edelman GM, Thiery JP (eds) The Cell in Contact. John Wiley and Sons, New York, pp 303–332

    Google Scholar 

  • Zalik SE, Scott V (1973) Sequential disappearance of cell surface components during dedifferentiation in lens regeneration. Nature New Biol 244:212–214

    Google Scholar 

  • Zalik SE, Scott V (1972) Cell surface changes during dedifferentiation in the metaplastic transformation of iris into lens. J Cell Biol 55:134–146

    Google Scholar 

  • Zardie L, Lisi A, Carnemolla B, Cosulich E, Viale G, Sanzi L (1980) A simplified procedure for the preparation of antibodies to serum fibronectin. J Immunol Method 34:155–165

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Zoology, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada

    Karen L. Elgert & Sara E. Zalik

Authors
  1. Karen L. Elgert
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sara E. Zalik
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This paper is dedicated to Dr. Tuneo Yamada on occasion of his 80th birthday

Rights and permissions

Reprints and permissions

About this article

Cite this article

Elgert, K.L., Zalik, S.E. Fibronectin distribution during cell type conversion in newt lens regeneration. Anat Embryol 180, 131–142 (1989). https://doi.org/10.1007/BF00309764

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00309764

Key words

Search

Navigation