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The formation of mesoderm and mesectoderm in presomite rat embryos cultured in vitro, using WGA-Au as a marker

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Summary

Presomite rat embryos cultured in vitro were injected with the cell marker wheat germ agglutinin-gold in order to find out whether the ectoderm already formed mesodermal cells. These labelled so-called mesectodermal cells were found in all embryos studied, ranging in age from 8.7 to 9.3 days post coitum. In embryos younger than 9.0 days, the entire head fold ectoderm produced mesectodermal cells. From 9.0 days onwards, the neural crest and surface ectoderm placodes were recognizable as separate entities, both producing mesectodermal cells. The early onset of mesectodermal cell formation and the numerous and continuous manufacture led us to the conclusion that mesectodermal cells are deposited at their definitive location and that subsequent long-distance migration is unnecessary.

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References

  • Adelmann HB (1925) The development of the neural folds and cranial ganglia in the rat. J Comp Neurol 39:19–171

    Google Scholar 

  • Barge JAJ (1918) Die Entwicklung der Craniovertabralgrenze beim Schaf. Anat H Abt I, 55:415–442

    Google Scholar 

  • Cobett P, Smithson KG, Hatton GI (1985) Dye-coupled magnocellular peptidergic neurons of the rat paraventricular nucleus show homotypic immunoreactivity. Neurosci 16:885–895

    Google Scholar 

  • Goedbloed JF (1972) The embryonic and postnatal growth of rat and mouse. I. Acta Anat 82:305–336

    Google Scholar 

  • Goedbloed JF, Smits-van Prooije AE (1986) A quantitative analysis of the temporal pattern of somite formation in mouse and rat. A simple and accurate method for age determination. Acta Anat 125:76–82

    Google Scholar 

  • Hamburger V, Hamilton HL (1953) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92

    Google Scholar 

  • Innes PB (1985) The ultrastructure of early cephalic neural crest cell migration in the mouse. Anat Embryol 172:33–38

    Google Scholar 

  • Johnston ME (1975) The neural crest in abnormalities of the face and brain. In: Bergsma D (ed) Morphogenesis and malformation of face and brain. The National Foundation — March of Dimes, vol XI, 7. Alan R Liss Inc, New York, pp 1–18

    Google Scholar 

  • Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol 27:137A

    Google Scholar 

  • Lamers CHJ, Rombout JWHM, Timmermans LPM (1981) An experimental study on neural crest migration in Barbus conchonius (Cyprinidae, Teleostei), with special reference to the origin of the enteroendocrine cells. J Embryol Exp Morphol 62:309–323

    Google Scholar 

  • Langman J (1963) Medical Embryology. The Williams and Wilkins Co., Baltimore

    Google Scholar 

  • Le Douarin NM (1982) The neural crest. Cambridge University Press, London New York

    Google Scholar 

  • Le Dourarin NM, Cochard P, Vincent M, Duband JL, Tucker GC, Teillet MA, Thiery JP (1984) Nuclear, cytoplasmic, and membrane markers to follow neural crest cell migration: a comparative study. In: Trelstad RL (ed) The role of extracellular matrix in development. Alan R Liss Inc, New York, pp 373–398

    Google Scholar 

  • Lumsden AG (1984) Tooth-forming potential of mammalian neural crest. J Embryol Exp Morphol [Suppl] 82:68

    Google Scholar 

  • Morriss GM, Thorogood PV (1978) An approach to cranial neural crest cell migration and differentiation in mammalian embryos. In: Johnson MH (ed) Development of mammals. North Holland Publishing Co 3:363–412

  • Nichols DH (1981) Neural crest formation in the head of the mouse embryo as observed using a new histological technique. J Embryol Exp Morphol 64:105–120

    Google Scholar 

  • Nichols DH (1986) Formation and distribution of neural crest mesenchyme to the first branchial arch region of the mouse embryo. Am J Anat 176:221–231

    Google Scholar 

  • Noden DM (1973) The migratory behavior of neural crest cells. In: Bosma JF (ed) Development in the fetus and infant, 4th symposiu on oral sensation and perception. DHEW Publ No (NIH) 73-546. Bethesda, Maryland, pp 9–33

  • Noden DM (1984) Craniofacial development: new views on old problems. Anat Rec 208:1–13

    Google Scholar 

  • O'Rahilly R, Müller F (1981) The first appearance of the human nervous system at stage 8. Anat Embryol 163:1–13

    Google Scholar 

  • Poelmann RE (1980) Differential mitosis and degeneration patterns in relation to alterations in shape of the embryonic ectoderm of early post-implantation mouse embryos. J Embryol Exp Morphol 55:33–51

    Google Scholar 

  • Poelmann RE (1981) The head-process and the formation of the definitive endoderm in the mouse embryo. Anat Embryol 162:41–49

    Google Scholar 

  • Shepard TH, Tanimura T, Robkin MA (1970) Energy metabolism in early mammalian embryos. Dev Biol [Suppl] 4:42–58

    Google Scholar 

  • Smits-van Prooije AE, Poelmann RE, Vermeij-Keers Chr (1984) Lectin-coated colloidal gold as a marker for neural crest cells in mammalian embryos, cultured in vitro. Ultramicroscopy 14:402

    Google Scholar 

  • Smits-van Prooije AE, Vemeij-Keers Chr, Poelmann RE, Mentink MMT, Dubbeldam JA (1985) The neural crest in presomite to 40-somite murine embryos. Act Morphol Neerl Scand 23:99–114

    Google Scholar 

  • Smits-van Prooije AE, Poelmann RE, Dubbeldam JA, Dubbeldam JA, Mentink MMT, Vermeij-Keers Chr (1986a) WGA-Au as a novel marker for mesectoderm formation in mouse embryos cultured in vitro. Stain Technol 61:97–106

    Google Scholar 

  • Smits-van Prooije A, Poelmann RE, Mentink M, Dubbeldam J, Vermeij-Keers Chr (1986b) Lectin coated colloidal gold as a marker for mesoderm formation in mammalian embryos, cultured in vitro. Acta Histochem [Suppl], vol XXXII, pp 175–180

    Google Scholar 

  • Smits-van Prooije AE, Vermeij-Keers Chr, Dubbeldam JA, Mentink MMT, Poelmann RE (1987) The formation of mesoderm and mesectoderm in somite stage rat embryos cultured in vitro, using WGA-Au as a marker. Anat Embryol 175: submitted

  • Snow MHL (1977) Gastrulation in the mouse: growth and regionalization of the epiblast. J Embryol Exp Morphol 42:292–303

    Google Scholar 

  • Stone LS (1929) Experiments showing the role of migrating neural crest (mesectoderm) in the formation of head skeleton and loose connective tissue in Rana palustris. Wilhelm Roux' Arch Entw Mech 118:40–77

    Google Scholar 

  • Thiery JP, Duband JL, Delouvée A (1982) Pathways and mechanisms of avian trunk neural crest cell migration and localization. Dev Biol 93:324–344

    Google Scholar 

  • Vermeij-Keers Chr, Poelmann RE (1980) The neural crest: a study on cell degeneration and the improbability of cell migration in mouse embryos. Neth J Zool 30:74–81

    Google Scholar 

  • Weston JA, Ciment G, Girdlestone J (1984) The role of the extracellular matrix in neural crest development: a reevaluation. In: Trelstad RL (ed) The role of extracellular matrix in development. Alan R Liss Inc, New York, pp 433–460

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy and Embryology, State University of Leiden, Wassenaarseweg 62, NL-2300 AL, Leiden, The Netherlands

    A. E. Smits-van Prooije, Chr. Vermeij-Keers, J. A. Dubbeldam, M. M. T. Mentink & R. E. Poelmann

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  1. A. E. Smits-van Prooije
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  2. Chr. Vermeij-Keers
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  3. J. A. Dubbeldam
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  4. M. M. T. Mentink
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  5. R. E. Poelmann
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Smits-van Prooije, A.E., Vermeij-Keers, C., Dubbeldam, J.A. et al. The formation of mesoderm and mesectoderm in presomite rat embryos cultured in vitro, using WGA-Au as a marker. Anat Embryol 176, 71–77 (1987). https://doi.org/10.1007/BF00309754

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