Abstract
The molecular weights calculated from the amino acid sequences of the αA and αB chains of the lens protein α-crystallin differ only slightly (19830 and 20070, respectively). SDS gel electrophoresis of these chains and comparison with marker proteins yield apparent molecular weights of 19500 for αA and 22500 for αB. The discrepancy between the value of 22500 and the real molecular weight of 20070 for αB vanishes by the combined use of SDS and 6 M urea in the polyacrylamide gels.
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Van Der Ouderaa, F.J.G., De Jong, W.W. & Bloemendal, H. The molecular weight of the basic polypetide chain αB2 of α-crystallin. Mol Biol Rep 1, 365–367 (1974). https://doi.org/10.1007/BF00309571
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DOI: https://doi.org/10.1007/BF00309571