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Isolation and characterization of a 5S RNA-protein complex from human placental ribosomes

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Abstract

Large ribosomal subunits treated with EDTA change their sedimentation rate in sucrose gradients and lose the 5S RNA molecule, which is released in a ribonucleoprotein particle sedimenting at about 7S. The proteins bound in this complex were identified by two-dimensional (2-D) polyacrylamide gel electrophoresis as ribosomal proteins L3 and L4, both having a molecular weight of about 37000.

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Authors and Affiliations

  1. Afdeling Biochemie, Department Humane Biologie, Fakulteit Geneeskunde, Katholieke Universiteit Leuven, B-300, Leuven, Belgium

    B. Peeters, L. Vanduffel & W. Rombauts

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  1. B. Peeters
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  2. L. Vanduffel
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  3. W. Rombauts
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Peeters, B., Vanduffel, L. & Rombauts, W. Isolation and characterization of a 5S RNA-protein complex from human placental ribosomes. Mol Biol Rep 1, 349–354 (1974). https://doi.org/10.1007/BF00309569

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  • DOI: https://doi.org/10.1007/BF00309569

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