Abstract
The interaction of netropsin with DNA and synthetic polydeoxyribonucleotides was studied by absorption spectrophotometry and circular dichroism. The results are consistent with a model in which a netropsin molecule occupies five base pairs in binding and carries three reaction sites each capable of interacting with one AT base pair. We associate these reaction sites with the antibiotic peptide groups which probably interact with AT base pairs by a hydrogen bonding mechanism.
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Schabel, F. M., Laster, W. R., Brockman, R. W., and Skipper, H. E., Proc. Soc. Exp. Biol. Med. 83, 1 (1953).
Zimmer, C. H., Luck, G., Thrum, H., and Pitra, C. H., Eur. J. Biochem. 26, 81 (1972).
Chandra, P., Zimmer, C. H., and Thrum, H., FEBS Letters 7, 90 (1970).
Zimmer, C. H., Reinert, K. E., Luck, G., Wähnert, U., Löber, G., and Thrum, H., J. Mol. Biol. 58, 329 (1971).
Reinert, K. E., J. Mol. Biol. 72, 593 (1972).
Krey, A. K., Alison, R. G., and Hahn, FEBS Letters 29, 58 (1973).
Thrum, H., Naturwissenschaften 46, 87 (1959).
Beabealaschvilly, R. S. and Savotchkina, L. P., Mol. Biol. U.S.S.R. 7, 93 (1973).
Brahms, J. and Mommaerts, W. H. M., J. Mol. Biol. 10, 73 (1964).
Ivanov, V. I., Minchenkova, L. E., Scholkina, A. K., and Poletayev, A. I., Biopolymers 12, 89 (1973).
Zasedatelev, A. S., Gursky, G. V., Zimmer, C. H., and Thrum, H., in preparation.
Zasedatelev, A. S., Gursky, G. V., and Volkenstein, M. V., Studia Biophysica 40, 79 (1973).
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Zasedatelev, A.S., Gursky, G.V., Zimmer, C. et al. Binding of netropsin to DNA and synthetic polynucleotides. Mol Biol Rep 1, 337–342 (1974). https://doi.org/10.1007/BF00309567
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DOI: https://doi.org/10.1007/BF00309567