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Requirements for the initiation of polyphenylalanine synthesis by recombined ribosomal subunits from yeast

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Abstract

A reaction stimulated by elongation factor 2 (EF-2) is necessary for the formation of the initial peptide-bond in poly(U)-directed peptide synthesis on yeast ribosomal subunits. The stimulation is inhibited by fusidic acid or diphtheria toxin together with NAD. It is assumed that the reaction of EF-2 represents translocation: in the presence of EF-2 almost twice the amount of N-acetyl-phenylalanyl-tRNA is bound to ribosomes and moreover, upon addition of puromycin a considerable stimulation of N-acetyl-phenylalanyl-puromycin formation is found. Contradictory data in the literature on this subject may be due to nonenzymatic translocation caused by a high monovalent cation concentration in the ribosome preparations or to an unspecific method for the extraction of N-acetyl-phenylalanyl-puromycin.

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Author notes

  1. Marleen H. Roos

    Present address: Dept. of Endocrinology, Academic Hospital of Utrecht, The Netherlands

  2. Ben A. M. Van Der Zeijst

    Present address: Institute of Virology, Veterinary Faculty, University of Utrecht, The Netherlands

  3. Henri P. J. Bloemers

    Present address: Dept. of Biochemistry, University of Nijmegen, Nijmegen, The Netherlands

Authors and Affiliations

  1. Van 't Hoff Laboratory, Biophysical Chemistry Department of the University of Utrecht, Transitorium III, Utrecht, The Netherlands

    Martyn H. Pranger, Marleen H. Roos, Ben A. M. Van Der Zeijst & Henri P. J. Bloemers

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  1. Martyn H. Pranger
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  2. Marleen H. Roos
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  3. Ben A. M. Van Der Zeijst
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  4. Henri P. J. Bloemers
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Pranger, M.H., Roos, M.H., Van Der Zeijst, B.A.M. et al. Requirements for the initiation of polyphenylalanine synthesis by recombined ribosomal subunits from yeast. Mol Biol Rep 1, 321–327 (1974). https://doi.org/10.1007/BF00309565

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  • DOI: https://doi.org/10.1007/BF00309565

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