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Biological activity of 125iodine labelled globin mRNA in an Ehrlich ascites cell-free system

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Abstract

Globin mRNA was isolated from avian erythroblasts and labelled with 125iodine to a specific activity of 4.5×106 dpm μg-1 mRNA. The labelling procedure was modified as compared to common techniques in order to protect the mRNA against degradation. When this RNA is translated in an Ehrlich ascites cell-free system globin chains are synthesized as is demonstrated by the analysis of the in vitro synthesized products. The biological activity of 125iodine labelled mRNA has proven to be 30% of that of unlabelled mRNA.

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Abbreviations

EDTA:

ethylenediamine tetraacetic acid

SDS:

sodium dodecyl sulfate

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Authors and Affiliations

  1. Institut für Molekularbiologie und Biochemie, Freie Universität, Berlin, Germany

    Walter Knöchel

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  1. Walter Knöchel
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Knöchel, W. Biological activity of 125iodine labelled globin mRNA in an Ehrlich ascites cell-free system. Mol Biol Rep 1, 311–320 (1974). https://doi.org/10.1007/BF00309564

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  • DOI: https://doi.org/10.1007/BF00309564

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