Summary
Using techniques of starch gel zone electrophoresis, a considerable variation among esterase and leucine aminopeptidase isoenzyme patterns was found in the endosperm of dormant seeds of Norway spruce (Picea abies). Since the so-called primary endosperm of conifers is only a further developmental stage of the haploid female gametophyte, simple Mendelian segregations can be determined in seeds of individual open-pollinated trees. It was therefore possible to identify some esterase and leucine aminopeptidase loci only with regard to phenotypic frequency distributions without difficult crossing procedures.
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Bergmann, F. Genetische Untersuchungen bei Picea abies mit Hilfe der Isoenzym-Identifizierung. Theoret. Appl. Genetics 43, 222–225 (1973). https://doi.org/10.1007/BF00309137
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DOI: https://doi.org/10.1007/BF00309137