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Effect of bleomycin on the fine structure of mouse fibroblasts

Veränderungen der Ultrastruktur von Mäusefibroblasten unter dem Einfluß von Bleomycin

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Summary

Asynchronously dividing mouse fibroblasts (L-cells) treated with the antitumor antibiotic Bleomycin show various rather specific morphological alterations. Many of the cells exposed to bleomycin assume a more or less epitheloid cell shape and are larger than untreated cells; in addition to an increase in nuclear size these cells often contain multiple nuclei. In most of the cells nuclei show an almost complete loss of peripheral condensed chromatin. The nucleolar hypertrophy initially observed is followed by a shrinkage and segregation of the nucleolar components.

The cytoplasmic alterations include dilatation of the cisternae of the rough endoplasmic reticulum as well as an increase of free, non membrane attached ribosomes, often arranged in spiral- and rosette-shaped polysemes; they are not specific for bleomycin.

Zusammenfassung

Asynchron wachsende Mäusefibroblasten (L-Zellen) ändern unter der Einwirkung von Bleomycin ihre typische Zellform. In den vergrößerten, teilweise epitheloiden Zellen finden sich häufig multiple Zellkerne; die Nucleolen sind vergrößert. Eine langdauernde Bleomycin-Behandlung führt hingegen zu einer Verkleinerung der Nucleolen, die häufig mit einer Segregation der nucleolären Bausteine einhergeht. Die auffallendsten ultrastrukturellen Veränderungen bestehen in einem nahezu vollständigen Verlust besonders des kondensierten randständigen Kernchromatins, in einer starken Erweiterung der Cysternen des rauhen endoplasmatischen Reticulums sowie in einer Zunahme der freien, nicht membrangebunden Ribosomen.

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Authors and Affiliations

  1. Pathologisches Institut (Ludwig-Aschoff-Haus) der Universität Freiburg i. Br., Bundesrepublik Deutschland

    H. Madreiter, R. Osieka, P. Kaden, A. Rombach & C. Mittermayer

  2. Innere Univ.-Klinik und Poliklinik (Tumorforschung), Klinikum Essen der Gesamthochschule Essen, Bundesrepublik Deutschland

    H. Madreiter, R. Osieka, P. Kaden, A. Rombach & C. Mittermayer

  3. Univ.-Kinderklinik/Laboratorien, A-6020, Innsbruck, Anichstraße 35, Österreich

    H. Madreiter

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  1. H. Madreiter
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Madreiter, H., Osieka, R., Kaden, P. et al. Effect of bleomycin on the fine structure of mouse fibroblasts. Z. Krebsforsch. 85, 63–72 (1976). https://doi.org/10.1007/BF00308130

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  • DOI: https://doi.org/10.1007/BF00308130

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