Summary
With autoradiography after labelling with tritiated thymidine, the kinetics of zymogen and parietal cells were studied in the gastric mucosa of mice. After one intraperitoneal injection of the DNA precursor, zymogen cells in the DNA synthesis phase were clearly identified on autoradiograms, whereas no parietal cells were seen to synthesize DNA.
In another group of mice, multiple injections were used in order to obtain a greater number of labelled cells. Following the latter procedure, analysis of grain count distributions over labelled zymogen cells and of labelling indices allowed detection of two subsequent zymogen cell divisions within an interval of approximately two months. This indicates that the cell turnover of zymogen cells is at least partly assured by their own mitotic activity.
By contrast, parietal cells showed no evidence of cell division, but appeared to be derived through differentiation from other cells in the neck area of the gland. Analysis of spatial distribution of the labelled parietal cells in the glandular tube indicated that, in time, most newly formed parietal cells undergo a slow migration directed downwards to the bottom of the fundic glands.
These results clearly show that the zymogen and the parietal cell population of the fundic glands have a different kinetic behaviour.
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References
Bensley, R. R.: In Cowdry's special cytology, vol. I: The gastric glands, p. 197. New York: Paul B. Hoeber Inc. 1932.
Bonfils, S., Lewin, M., Vatier, J., Dubrasquet, M., Bader, J. P.: Correlation between acid and peptic secretion in man (K25). In: The physiology of gastric secretion, p. 164–173, L. S. Semb and J. Myren, eds. Oslo: Universitätsvorlaget 1968.
Bowie, D. J.: A method for staining pepsinogen granules in gastric glands. Anat. Rec. 64, 357–368 (1936).
Chang, W. W. L., Leblond, C. P.: Renewal of the epithelium in the descending colon of the mouse. I. Presence of three cell populations: vacuolated-columnar, mucous and argentaffin. Amer. J. Anat. 131, 73–100 (1971).
Corpron, R. E.: The ultrastructure of the gastric mucosa in normal and hypophysectomized rats. Amer. J. Anat. 118, 53–90 (1966).
Creamer, B., Shorter, R. G., Bamforth, J.: The turnover and shedding of epithelial cells. I. The turnover in the gastrointestinal tract. Gut 2, 110–118 (1961).
Crean, G. P., Marshall, M. W., Rumsey, R. D. E.: Parietal cell hyperplasia of the gastric mucosa induced by the administration of pentagastrin to rats. Gastroenterology 57, 147–155 (1969).
Deschner, E. E., Lipkin, M.: An autoradiographic study of the renewal of argentaffin cells in human rectal mucosa. Exp. Cell Res. 43, 661–665 (1966).
Gerard, A.: Etudes histochimiques et histophysiologiques des glycoprotéines de la muqueuse gastrique chez le chien. Arch. Biol. (Liège) 79, 1–104 (1968).
Hughes, W. L., Bond, V. P., Brecker, G., Cronkite, E. P., Painter, R. B., Quastler, H., Sherman, F. G.: Cellular proliferation in the mouse as revealed by autoradiography with tritiated thymidine. Proc. nat. Acad. Sci. (Wash.) 44, 476–483 (1958).
Hunt, T. E., Hunt, E. A.: H3-thymidine autoradiographs of the gastric mucosa of the rat after stimulation with compound 48/80. Anat. Rec. 139, 240–241 (1961).
Hunt, T. E., Hunt, E. A.: Radioautographic study of proliferation in the stomach of the rat using H3-thymidine and compound 48/80. Anat. Rec. 142, 505–511 (1962).
Ito, S., Winchester, R.: The fine structure of the gastric mucosa in the bat. J. Cell Biol. 16, 541–577 (1963).
Lane, B. P., Europa, D. L.: Differential staining of ultrathin sections of epon-embedded tissues for light microscopy. J. Histochem. Cytochem. 13, 579–580 (1965).
Lawn, A. M.: Observations on the fine structure of the gastric parietal cell of the rat. J. biophys. biochem. Cytol. 7, 161–166 (1960).
Lawson, H. H.: The origin of chief and parietal cells in regenerating gastric mucosa. Brit. J. Surg. 57, 139–141 (1970).
Lillie, R. D.: Histopathologic technic and practical histochemistry, second edition. New York: McGraw Hill 1954.
Lipkin, M., Sherlock, P., Bell, B.: Cell proliferation kinetics in the gastrointestinal tract of man. II. Cell renewal in stomach, ileum, colon, rectum. Gastroenterology 45, 721–729 (1963).
MacDonald, W. C., Trier, J. S., Everett, N. B.: Cell proliferation and migration in the stomach, duodenum and rectum of man: radioautographic studies. Gastroenterology 46, 405–417 (1964).
Matsuyama, M., Susuki, H.: Differentiation of immature mucous cells into parietal, argyrophil and chief cells in stomach grafts. Science 169, 385–387 (1970).
Merzel, J.: Preparation of semi-thin serial sections of epon embedded material. Experientia (Basel) 27, 611–612 (1971).
Messier, B., Leblond, C. P.: Preparation of coated radioautographs by dipping sections in fluid emulsion. Proc. Soc. exp. Biol. (N.Y.) 96, 476–485 (1960).
Myrhe, E.: Regeneration of the fundic mucosa in rats. Arch. Path. 70, 476–485 (1960).
Ragins, H., Ditbrenner, H., Diaz, J.: Comparative histochemistry of the gastric mucosa: a survey of the common laboratory animals and man. Anat. Rec. 150, 179–194 (1964).
Ragins, H., Winckze, F., Liu, S. M., Ditbrenner, H.: The origin and survival of gastric parietal cells in the mouse. Anat. Rec. 162, 99–110 (1968).
Rubin, W., Ross, L. L., Sleisenger, M. H., Jeffries, G. H.: The normal human gastric epithelia. A fine structural study. Lab. Invest. 19, 598–626 (1968).
Samloff, I. M.: Cellular localization of group I pepsinogens in human gastric mucosa by immunofluorescence. Gastroenterology 61, 185–188 (1971).
Sawicki, W., Blaton, O., Rowinsky, J.: Correction of autoradiographic grain count in respect to precisely calculated background. Histochemie 26, 67–73 (1971).
Stevens, C. E., Leblond, C. P.: Renewal of the mucous cells in the gastric mucosa of the rat. Anat. Rec. 115, 231–246 (1953).
Traurig, H. H.: Cell proliferation in the mammary gland during late pregnancy and lactation. Anat. Rec. 157, 489–503 (1967).
Willems, G., Galand, P., Chretten, J.: Autoradiographic studies on cell population kinetics in dog gastric and rectal mucosa. A comparison between in vitro and in vivo methods. Lab. Invest. 23, 635–639 (1970).
Willems, G., Vansteenkiste, Y., Limbosch, J. M.: Stimulating effect of gastrin on cell proliferation kinetics in canine fundic mucosa. Gastroenterology 62, 583–589 (1972).
Willems, G., Vansteenkiste, Y., Smets, Ph.: Effects of food ingestion on the cell proliferation kinetics in the canine fundic mucosa. Gastroenterology 61, 323–327 (1971).
Willems, G., Vansteenkiste, Y., Verbeustel, S.: Autoradiographic study of cell renewal in fundic mucosa of fasting dogs. Acta anat. (Basel) 80, 23–32 (1971).
Zeitoun, P., Potet, F., Zylberberg, E.: In: L'antre gastrique (1 vol.) Histologie de la muqueuse antrale, p. 10–18. Paris: Masson, édit. 1969.
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This work was supported by a grant of the “Nationaal Fonds voor Wetenschappelijk Onderzoek”.
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Willems, G., Galand, P., Vansteenkiste, Y. et al. Cell population kinetics of zymogen and parietal cells in the stomach of mice. Z.Zellforsch 134, 505–518 (1972). https://doi.org/10.1007/BF00307670
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DOI: https://doi.org/10.1007/BF00307670