Summary
Succinate-, α-glycerophosphate-, NADH-, and NADPH dehydrogenases (tetrazolium reductases) were studied histochemically in brain sections. The distribution of these enzymes in the hippocampal region was mapped in different postnatal stages. In addition to the hippocampus and the dentate area which have also been dealt with by other authors, the description includes the entorhinal area, parasubiculum, presubiculum, and subiculum.
In all the areas of the hippocampal region the enzyme activity is first localized mainly in cell bodies. Activity in the neuropil shows a tendency to increase with age throughout the stages studied. Examples of particularly early or late differentiation of the histochemical pattern exist. Thus already after 4–6 days the differentiated staining pattern in the hippocampal layers as seen in the adult, begins to appear. In the entorhinal area the early enzyme distribution agrees with that in the adult and facilitates identification of its layers. After 22 days the fundamental enzyme chemoarchitectonics of the adult rat brain has been obtained. Histochemical characteristics of the different areas, support the view that the hippocampal region constitutes an entity.
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Valuable advice and suggestions on the manuscipt by Professor T. W. Blackstad, Århus, Denmark are greatfully acknowledged. The author is indebted to Miss L. Skarstein, Miss V. Neeson, Mr. R. Jensen and Mr. R. Haakonsen for technical assistance.
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Mellgren, S.I. Distribution of succinate-, α-glycerophosphate-, NADH-, and NADPH dehydrogenases (tetrazolium reductases) in the hippocampal region of the rat during postnatal development. Z.Zellforsch 141, 347–373 (1973). https://doi.org/10.1007/BF00307411
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DOI: https://doi.org/10.1007/BF00307411