Summary
The accessory cells of rat, guinea-pig and chick sympathetic ganglia have been studied in tissue culture with phase-contrast microscopy, time-lapse cinematography and fluorescence histochemistry. Small intensely fluorescent (SIF) cells have been described for the first time in culture. Satellite cells, which are closely associated with nerve cell bodies, and interstitial cells, which are dispersed throughout the ganglion, appeared to be identical cell types; they do not correspond to oligodendrocytes of the central nervous system as has been suggested previously. However, a small number of cells closely resembling central oligodendrocytes were present. Astrocyte-like cells have also been seen occasionally, although their identity is not clear. Several other cell types were present and have been identified as Schwann cells, sheath cells, fibroblasts, perineural epithelium, macrophages and endothelial cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bennett, T., Malmfors, T.: The adrenergic nervous system of the domestic fowl (Gallus domesticus). Z. Zellforsch. 106, 22–50 (1970).
Bertrand, I., Guillain, J.: La microglie et l'oligodendroglie ganglionaires. C. R. Soc. Biol. (Paris) 113, 382–383 (1933).
Botár, J.: The autonomic nervous system, p. 183–238. Budapest: Akadémiai Kiadó 1966.
Brizzee, K. R.: Histogenesis of the supporting tissue in the spinal and the sympathetic trunk ganglia in the chick. J. comp. Neurol. 91, 129–146 (1949).
Cajal, Ramón, Y. S.: Notas preventivas sobre el gran simpático y retina de los mamiferos. Graceto Sanitaria de Barcelona 10 déc (1891).
Cajal, Ramón, Y. S.: Histologie du système nerveux de l'homme et des vertébres. Paris: A. Maloine 1911.
Castro, F. de: Sympathetic ganglia, normal and pathological. In: Cytology and cellular pathology of the nervous system, ed. Penfield, W., p. 317–379. New York: P. B. Hoeber 1932.
Chamley, J. H., Mark, G. E., Campbell, G. R., Burnstock, G.: Sympathetic ganglia in culture. I. Neurons. Z. Zellforsch. 135, 287–314 (1972).
Costa, M.: Observations on extra-adrenal chromaffin tissue. In: Second International Meeting of the International Society for Neurochemistry, eds. Paoletti, R., Fumagalli, R., and Galli, C., p. 136–137 (1969).
Eränkö, O., Härkönen, M.: Histochemical demonstration of fluorogenic amines in the cytoplasm of sympathetic ganglion cells of the rat. Acta physiol. scand. 58, 285–286 (1963).
Ernyei, S., Young, M. R.: Pulsatile and myelin activities of Schwann cells in vitro. J. Physiol. (Lond.) 183, 469–480 (1966).
Falck, B.: Observations on the possibilities of the cellular localisation of monoamines by a fluorescence method. Acta physiol. scand. 56, Suppl. 197 (1962).
Jacoby, F.: Macrophages. In: Cells and tissues in culture. Methods, biology and physiology, ed. Willmer, E. N., vol. 2, p. 1–93. London-New York: Academic Press 1965.
Kuntz, A., Sulkin, N. M.: The neuroglia in the autonomic ganglia: cytologic structure and reactions to stimulation. J. comp. Neurol. 86, 467–477 (1947).
Lumsden, C. E., Pomerat, C. M.: Normal oligodendrocytes in tissue culture: A preliminary report on the pulsatile glial cells in tissue cultures from the corpus callosum of the normal adult rat brain. Exp. Cell Res. 2, 103–114 (1951).
Matthews, M. R., Raisman, G.: The ultrastructural and somatic efferent synapses of small granule-containing cells in the superior cervical ganglion. J. Anat. (Lond.) 105, 255–282 (1969).
Murray, M. R., Stout, A. P.: Adult human ganglion cells cultivated in vitro. Amer. J. Anat. 80, 225–273 (1947).
Nakai, J.: Transformations and multiplication of neuroglia in tissue culture. In: Proc. IV. International Congress of Neuropath, Munich, ed. Jacob, H., vol. 2, p. 241–246. Stuttgart: Georg Thieme 1962.
Nakai, J., Okamoto, M.: Identification of neuroglial cells in tissue culture. In: Morphology of neuroglia, ed. Nakai, J., Igaku Shoin Ltd. Tokyo Japan, p. 65–102. Springfield, Illinois: Charles C. Thomas 1963.
Orden, L. S. van, Burke, J. P., Geyer, M., Lodoen, F. V.: Localisation of depletion-sensitive and depletion-resistant noradrenaline storage sites in autonomic ganglia. J. Pharmacol. exp. Ther. 174, 357–368 (1970).
Pick, J.: The autonomic nervous system. Morphological, comparative, clinical and surgical aspects. Philadelphia-Toronto: J. B. Lippincott Co. 1970.
Pomerat, C. M.: Pulsatile activity of cells from the human brain in tissue culture. J. nerv. ment. Dis. 114, 430–449 (1951).
Pomerat, C. M.: Functional concepts based on tissue culture studies of neuroglia cells. In: Biology of Neuroglia, ed. Windle, W. F., p. 162–175. Springfield, Illinois: Charles C. Thomas 1958.
Pomerat, C. M.: Rhythmic contraction of Schwann cells. Science 130, 1759–1760 (1959).
Rio-Hortega, P. del, Prado, J. M.: Estudios sobre la neuroglia periférica. I. La neuroglia de los ganglios simpaticos. Rev. Soc. argent. Biol. 17, 512–521 (1941).
Sano, K., Satoh, F., Chigasaki, H.: Tissue culture of glioma. In: Morphology of neuroglia, ed. Nakai, J., p. 133–150. Springfield, Illinois, Charles C. Thomas 1963.
Shanthaveerappa, T. R., Bourne, G. H.: The perineural epithelium of sympathetic nerves and ganglia and its relation to the pia arachnoid of the central nervous system and perineural epithelium of the peripheral nervous system. Z. Zellforsch. 61, 742–753 (1964).
Varon, S. S., Raiborn, C. W., Jr., Seto, T., Pomerat, C. M.: A cell line from trypsinized adult rabbit brain tissue. Z. Zellforsch. 59, 35–46 (1963).
Author information
Authors and Affiliations
Additional information
This work was supported by the National Heart Foundation of Australia and the Australian Research Grants Committee. We are particularly grateful to Janet McConnell for her excellent technical assistance.
Rights and permissions
About this article
Cite this article
Chamley, J.H., Mark, G.E. & Burnstock, G. Sympathetic ganglia in culture. Z.Zellforsch 135, 315–327 (1972). https://doi.org/10.1007/BF00307179
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00307179