Summary
The development of annulate lamellae (AL) in the rabbit zygote has been described previously (Gulyas, 1971). The present study is a continuation of the earlier work on the fate of AL during first cleavage. Intranuclear AL (IAL) reappear during chromosome condensation which occurs at the proximal regions of the pronuclei. Chromosomes become attached to the convoluted pronuclear envelope (PNE) and the newly forming IAL. Both the PNEs and the cytoplasmical undergo similar alterations although the PNE precedes that of the AL. The pores disappear from the PNEs prior to their breakdown, and paired cisternae of PNE origin are observed throughout the remaining phases of division. Just prior to and during breakdown of the PNE, pores disappear from AL and the lamellae coalesce into paired and multilamellar cisternae. Electron dense substance remains deposited between some partially coalesced cisternae at periodic intervals, giving them a beaded appearance. Clusters of punctate material accompany the paired and multilamellar cisternae throughout division. Paired and multilamellar cisternae, which are accompanied by punctate electron dense substance, are seen throughout division including early interphase of the daughter cells. These cisternae do not participate in the early formation of NE. Annulate lamellae are reformed from paired and multilamellar cisternae during late telophase and early interphase. New AL are also formed from vesicles of the nuclear envelope of the blastomere nucleus.
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The author is most grateful to Drs. Daniel Szollosi, Griff Ross and William Tullner for critically reviewing the manuscript.
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Gulyas, B.J. The rabbit zygote. Z.Zellforsch 133, 187–200 (1972). https://doi.org/10.1007/BF00307141
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DOI: https://doi.org/10.1007/BF00307141