Summary
Prior to the onset of mitotic activity in the regenerating mouse liver, the concentrations of total protein and DNA, but not of RNA, decreased to 93 per cent of their levels in normal liver. During maximum mitosis (48–72 hours), the DNA and RNA concentrations were 110 per cent of their normal levels. The concentrations of liver nucleic acids 24 hours after a sham-operation were also about 110 per cent of normal values.
Increased concentrations of insoluble protein were found at 12, 24 and 144 hours after partial hepatectomy. This may reflect an increase in the stability of the liver cell membranes during the regenerative period. Increased amounts of various cytoplasmic membranes were indicated by electronmicroscopy and may also have contributed to the increase in insoluble protein.
A temporary increase in the measured solubility of the liver protein occurred during maximum mitosis. It is suggested that this was due to the association of protein with lipids during the depletion of accumulated fat from the regenerating liver.
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References
Bartók, I., Totovic, V., Gedigk, P.: Über die Entstehung der peribiliären dichten Körper der Leberzellen. Virchows Arch. path. Anat. 343, 1–19 (1967).
Becker, F. F., Lane, B. P.: Regeneration of the mammalian liver. VI. Retention of phenobarbital-induced cytoplasmic alterations in dividing hepatocytes. Amer. J. Path. 52, 211–225 (1968).
Bengmark, S.: Liver steatosis and liver resection. Digestion 2, 304–311 (1969).
Bengmark, S.: Liver regeneration. In: Tumours of the liver, ed. by Pack, G. T., and Islami, A. H., p. 187–212. Berlin-Heidelberg-New York: Springer 1970.
Bucher, N. L. R.: Regeneration of the mammalian liver. Int. Rev. Cytol. 15, 245–300 (1963).
Bucher, N. L. R.: Exp. aspects of hepatic regeneration. New Engl. J. Med. 277, 686–696, 738–746 (1967).
Bucher, N. L. R., Swaffield, M. N.: Ribonucleic acid synthesis in relation to precursor pools in regenerating rat liver. BBA 174, 491–502 (1969).
Dougherty, T. F.: Effect of hormones on lymphatic tissue. Physiol. Rev. 32, 379–401 (1952).
Enwonvu, C. O., Stambaugh, R., Sreebny, L.: Synthesis and degradation of liver ribosomal RNA in fed and fasted rats. J. Nutr. 101, 337–345 (1971).
Grisham, J. W.: A morphologic study of deoxyribonucleic acid synthesis and cell proliferation in regenerating rat liver; Autoradiography with thymidine-H3. Cancer Res. 22, 842–849 (1962).
Grundmann, E., Seidel, H. J.: Die reparative Parenchymregeneration am Beispiel der Leber nach Teilhepatektomie. In: Handbuch der allgemeinen Pathologie, VI/2, hrsg. von Büchner, F., S. 129–243. Berlin-Heidelberg-New York: Springer 1969.
Heby, O., Lewan, L.: Putrescine and polyamines in relation to nucleic acids in mouse liver after partial hepatectomy. Virchows Arch. Abt. B 8, 58–66 (1971).
Higgins, G. M., Andersson, R. M.: Experimental pathology of the liver. Arch. Path. 12, 186–202 (1931).
Husáková, A., Simek, J.: Changes in the composition of serum beta-lipoproteins in rats after partial hepatectomy and adrenalectomy. Physiol. bohemoslov. 19, 95–100 (1970).
Jardetszky, C. D., Barnum, C. P., Vermund, H.: Deoxyribonucleic acid and phospholipide metabolism in regenerating liver and the effect of x-radiation. J. biol. Chem. 222, 421–433 (1956).
Jordan, S. W.: Electron microscopy of hepatic regeneration. Exp. molec. Path. 3, 183–200 (1964).
Lancker, J. L. van, Sempoux, D. G.: Regeneration of various cellular components in rat livers after subtotal hepatectomy. Arch. Biochem. 77, 129–137 (1958).
Lewan, L.: Nuclear proliferation and the amount of DNA per parenchymal nucleus during mouse liver regeneration. Virchows Arch. Abt. B 9, 271–279 (1971).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951). J. biol. Chem. 193, 265–275 (1951).
Luft, J. H.: Permanganate—a new fixative for electron microscopy. J. biophys. biochem. Cytol. 2, 799–802 (1956).
Majumdar, C., Tsukuda, K., Lieberman, I.: Liver protein synthesis after partial hepatectomy and acute stress. J. biol. Chem. 242, 700–704 (1967).
Morgan, E. H., Brackenridge, C. J.: Serum protein changes and plasma total iron binding capacity after laparatomy or partial hepatectomy and in anaemia in the albino rat. Quart. J. exp. Physiol. 47, 66–73 (1962).
Munro, H. N., Fleck, A.: Recent developments in the measurement of nucleic acids in biological materials. Analyst 91, 78–88 (1966).
Schreiber, G., Urban, J., Zähringer, J., Reuter, W., Frosch, U.: The secretion of serum protein and the synthesis of albumin and total protein in regenerating rat liver. J. biol. Chem. 240, 4531–4538 (1971).
Trotter, N. L.: A fine structure study of lipid in mouse liver regenerating after partial hepatectomy. J. Cell Biol. 21, 233–244 (1964).
Tsuboi, K. K., Yokoyama, H. O., Stowell, R. E., Wilson, M. E.: The chemical composition of regenerating mouse liver. Arch. Biochem. 48, 275–292 (1954).
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This investigation was facilitated by grants from the Royal. Physiographical Society.
I wish to thank Professor I. Agrell and Docent B. Karlsson for helpful advice and criticism and Miss E. K. Persson for skilled technical assistance.
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Lewan, L. Protein, nucleic acids and cell structure in the regenerating mouse liver. Z. Zellforsch 129, 56–64 (1972). https://doi.org/10.1007/BF00307110
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DOI: https://doi.org/10.1007/BF00307110