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Summary

A light and electron microscopic autoradiographic analysis revealed that H3-valine infused into the lateral ventricle of normal and acutely dehydrated cats is preferentially taken up by the supraoptic (SON) and paraventricular nucleus (PVN) of the hypothalamus. Grain counts for these magnocellular neurons in normal unstressed cats were highest at one hour post infusion with a significant fall off by three hours. Uptake by the SON and PVN at one hour exceeded neighboring nuclear groups by a factor of 7 and 4 fold respectively. Electron microscopic autoradiographs from acutely dehydrated cats revealed the presence of emission grains in association with rough endoplasmic reticulum and large osmiophilic neurosecretory vesicles. In view of statistically significant uptake values and rapid turnover of H3-valine by SON and PVN in normal animals, coupled with emission tracks in direct association with underlying neurosecretory product in acutely dehydrated ones, it is speculated that valine may be an amino acid component of one or both of the neurophysins to which neurohypophyseal hormones are non-covalently linked.

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References

  • Akmayev, I. G.: Morphologic aspects of the hypothalamic-hypophyseal system. II. Functional morphology of pituitary microcirculation. Z. Zellforsch. 116, 178–194 (1971).

    Google Scholar 

  • Bachmann, L., Salpeter, M. M.: Absolute sensitivity of electron microscope radioautography. J. Cell Biol. 33, 299–305 (1967).

    Google Scholar 

  • Bargmann, W.: The neurosecretory system of the diencephalon. Endeavour 19, 125–133 (1960).

    Google Scholar 

  • Bargmann, W., Knoop, A.: Elektronenmikroskopische Beobachtungen an der Neurohypophyse. Z. Zellforsch. 46, 242–251 (1957).

    Google Scholar 

  • Bergland, R. M., Torack, R. M.: An electron microscopic study of the human infundibulum. Z. Zellforsch. 99, 1–12 (1969).

    Google Scholar 

  • Bleier, R.: The hypothalamus of the cat: a cytoarchitectonic atlas with Horsley-Clark coordinates, p. 1–109. Johns Hopkins Press 1961.

  • Brightman, M.: Personal Communication (1972).

  • Buford, G. D., Moens, L.: Isolation of rat neurohypophysial proteins and their tentative identification as neurophysins. J. Endocr. 51, 609–610 (1971).

    Google Scholar 

  • Caro, L. G., Tubergen, R. P. van: High-resolution autoradiography. I. Methods. J. Cell Biol. 15, 173–188 (1962).

    Google Scholar 

  • Cheng, K. W., Friesen, H. G.: Isolation and characterization of a third component of porcine neurophysin. J. biol. Chem. 246, 7656–7665 (1971).

    Google Scholar 

  • Duncan, D., Alexander, R.: An electron microscope study of the supraoptic nucleus of the rat. Anat. Rec. 139, 223 (1961).

    Google Scholar 

  • Du Vigneaud, V., Ressler, C., Swan, J. M., Roberts, C. W., Katsoyannis, P. G., Gordon, S.: The synthesis of an octapeptide amide with the hormonal activity of oxytocin. J. Amer. chem. Soc. 75, 4879–4880 (1953).

    Google Scholar 

  • Enestrom, S.: Nucleus supraopticus. A morphological and experimental study in the rat. Acta. path. microbiol. scand., Suppl. 186, 1–99 (1967).

    Google Scholar 

  • Ficq, A., Flament-Durand, J.: Autoradiography in endocrine research. In: Techniques in endocrine research (P. Eckstein and F. Knowles, eds.), p. 73–85. New York: Academic Press 1963.

    Google Scholar 

  • Finley, K. H.: Angio-architecture of the hypothalamus and its peculiarities. Res. Publ. Ass. nerv. ment. Dis. 20, 286–319 (1940).

    Google Scholar 

  • Gerschenfeld, H. M., Tramezzani, J. H., De Robertis, E.: Ultrastructure and function in neurohypophysis of the toad. Endocrinology 66, 741–762 (1960).

    Google Scholar 

  • Green, J. D., Maxwell, D. S.: Comparative anatomy of the hypophysis and observations on the mechanism of neurosecretion. In: Comparative endocrinology (A. Gorbman, ed.), p. 368–392. New York: Wiley 1959.

    Google Scholar 

  • Hartmann, J. F.: Electron microscopy of the neurohypophysis in normal and histamine-treated rats. Z. Zellforsch. 48, 291–308 (1958).

    Google Scholar 

  • Hayward, J. N.: Central neural regulation of antidiuretic hormone release and unit activity in the supraoptic nucleus of the behaving rhesus monkey. Amer. J. Anat. 129, 203–206 (1970).

    Google Scholar 

  • Hayward, J. N.: Effects of changes in carotid osmolality on behavior, EEG and hypothalamic single unit activity in the monkey. Fed. Proc. 29, 831 (1970b).

    Google Scholar 

  • Hayward, J. N., Vincent, J. D.: Activity of single hypothalamic neurons in behaving monkeys using a method for repeated intracarotid injections. Anat. Rec. 166, 317 (1970a).

    Google Scholar 

  • Jewell, P. A., Verney, E. B.: An experimental attempt to determine the site of the neurohypophyseal osmoreceptors in the dog. Phil. Trans. 240, 197–324 (1957).

    Google Scholar 

  • Karnovsky, M. J.: A formaldehyde glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol. 27, 137A-138A (1965).

    Google Scholar 

  • Kasten, F. H.: Personal Communication (1967).

  • Koizumi, K., Ishikawa, T., Brooks, C. Mc C.: Control of activity of neurons in the supraoptic nucleus. J. Neurophysiol. 27, 878–892 (1964).

    Google Scholar 

  • Kurosumi, K., Matsuzawa, T., Shibasaki, S.: Electron microscope studies on the fine structures of the pars nervosa and pars intermedia, and their morphological interrelation in the normal rat hypophysis. Gen. comp. Endocr. 1, 433–452 (1961).

    Google Scholar 

  • Lederis, K.: An electron microscopical study of the human neurohypophysis. Z. Zellforsch. 65, 847–868 (1965).

    Google Scholar 

  • Monroe, B. G., Scott, D. E.: Ultrastructural changes in the neural lobe of the hypophysis of the rat during lactation and suckling. J. Ultrastruct. Res. 14, 497–517 (1966).

    Google Scholar 

  • Murakami, M.: Elektronenmikroskopische Untersuchung der neurosekretorischen Zellen im Hypothalamus der Maus. Z. Zellforsch. 56, 277–299 (1962).

    Google Scholar 

  • Nibbelink, D. W.: Paraventricular nuclei, neurohypophysis, and parturition. Amer. J. Physiol. 200, 1229–1232 (1961).

    Google Scholar 

  • Norstrom, A., Sjöstrand, J.: Axonal transport of proteins in the hypothalamo-neurohypophysial system of the rat. J. Neurochem. 18, 29–39 (1971).

    Google Scholar 

  • Olivecrona, H.: Paraventricular nucleus and pituitary gland. Acta physiol. scand. 40, Suppl. 136, 1–178 (1957).

    Google Scholar 

  • Oota, Y.: The fine structure of the median eminence and pars nervosa of the mouse. J. Fac. Sci. Univ. Tokyo 10, 155–168 (1963).

    Google Scholar 

  • Palay, S. L.: Fine structure of the neurohypophysis. Progr. Neurobiol. 2, 31–49 (1957).

    Google Scholar 

  • Rechardt, L.: Electron microscopic and histochemical observations on the supraoptic nucleus of normal and dehydrated rats. Acta. physiol. scand., Suppl. 329, 1–79 (1969).

    Google Scholar 

  • Revel, J.-P., Hay, E. D.: An autoradiographic and electron microscopic study of collagen synthesis in differentiating cartilage. Z. Zellforsch. 61, 110–144 (1963).

    Google Scholar 

  • Rodríguez, E. M.: Hormonal content and ultrastructure of the disconnected neural lobe of the grass frog (Rana pipiens). Gen. comp. Endocr. 15, 272–288 (1970b).

    Google Scholar 

  • Rodríguez, E. M., Dellmann, H.-D.: Ultrastructure and hormonal content of the proximal stump of the transected hypothalamo-hypophysial tract of the frog (Rana pipiens). Z. Zellforsch. 104, 449–470 (1970a).

    Google Scholar 

  • Rodríguez, E. M., La Pointe, J.: Histology and ultrastructure of the neural lobe of the lizard, (Klauberina riversiana). Z. Zellforsch. 95, 35–57 (1969).

    Google Scholar 

  • Rose, W. C.: The nutritive significance of the amino acids. Physiol. Rev. 18, 109–136 (1938).

    Google Scholar 

  • Sachs, H.: Neurosecretion in the mammalian hypothalamo-neurohypophysial complex. Protides of the Biological Fluids 13, 181–192 (1966).

    Google Scholar 

  • Scharrer, B.: The role of neurosecretion in neuroendocrine integration. New York: Wiley & Sons 1958.

    Google Scholar 

  • Scharrer, E.: Die Lichtempfindlichkeit blinder Elritzen. (Untersuchungen über das Zwischenhirn der Fische I.) Z. vergl. Physiol. 7, 1–38 (1928).

    Google Scholar 

  • Scott, D. E.: Fine structural features of the neural lobe of the hypophysis of the rat with homozygous diabetes insipidus (Brattleboro strain). Neuroendocrinology 3, 156–176 (1968a).

    Google Scholar 

  • Scott, D. E.: An autoradiographic and electron microscopic investigation of the hypothalamo-neurohypophyseal system of normal rats, acutely dehydrated rats, and chronically dehydrated rats with diabetes insipidus. Thesis. Diss. Abst. 29, 24-B (1968b).

  • Scott, D. E.: Ultrastructural changes in the neural lobe of the hypophysis of the kangaroo rat (Dipodomys merriami) following intraperitoneal injection of hypertonic sodium chloride. Neuroendocrinology 4, 347–367 (1969).

    Google Scholar 

  • Scott, D. E., Dudley, G., Krobisch, Gibbs, F. P., Brown, G. M.: The mammalian median eminence. A comparative and experimental model. In: Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich, 1971 (K. M. Knigge, D. E. Scott and A. Weindl, eds.), p. 35–49. Basel: Karger 1972.

    Google Scholar 

  • Scott, D. E., Knigge, K. M., Dudley, G. Krobisch: The mammalian median eminence, a neuroendocrine transducer. Sci. Amer. In Press (1971).

  • Senchik, J. I., Polenov, A. L.: On lipid inclusions in the neurosecretory cells of the supraoptic nucleus of the white mouse subjected to a chronic salt load. Z. Zellforsch. 100, 118–125 (1969).

    Google Scholar 

  • Sloper, J. C., Arnott, D. J., King, B. C.: Sulphur metabolism in the pituitary and hypothalamus of the rat: a study of radioisotope-uptake after the injection of 35SDL-cysteine, methionine and sodium sulfate. J. Endocr. 20, 9–23 (1960).

    Google Scholar 

  • Sloper, J. C., Bateson, R. G.: Ultrastructure of neurosecretory cells in the supraoptic nucleus of the dog and rat. J. Endocr. 31, 139–150 (1965).

    Google Scholar 

  • Sokol, H. W., Valtin, H.: Evidence for the synthesis of oxytocin and vasopressin in separate neurons. Nature (Lond.) 214, 314–316 (1967).

    Google Scholar 

  • Streefkerk, J. G.: Functional changes in the morphological appearance of the hypothalamo-hypophyseal neurosecretory and catecholaminergic neural system, and in the adenohypophysis of the rat. Thesis. Amsterdam: Drukkerij Wed. G. van Soest. N. V. (1967).

    Google Scholar 

  • Talanti, S.: The incorporation of 35S-labelled cysteine in the hypothalamic-hypophyseal neurosecretory system of the dehydrated rat. Z. Zellforsch. 115, 110–113 (1971).

    Google Scholar 

  • Talanti, S., Attila, U., Kekki, M.: The kinetics of 35S-labelled cysteine in the hypothalamic-hypophyseal tract of the rat, studied by autoradiography. Z. Zellforsch. 124, 342–353 (1972).

    Google Scholar 

  • Talanti, S., Pasanen, V.: The incorporation of S35-labelled cysteine in the hypothalamic-hypophyseal neurosecretory system of rats treated with thiouracil and excess thyroxin. Z. Zellforsch. 88, 220–227 (1968).

    Google Scholar 

  • Van Dyke, H. B.: Vasopressins. Proc. XXI Int. Cong. Physiol. Sci. 61 (1959).

  • Venable, J. H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25, 407–408 (1965).

    Google Scholar 

  • Verney, E. B.: The antidiuretic hormone and the factors which determine its release. Proc. roy. Soc. B 135, 25–106 (1947).

    Google Scholar 

  • Weindl, A., Joynt, R. J.: The median eminence as a circumventricular organ. In: Brainendocrine interaction. Median eminence: Structure and function. Int. Symp. Munich, 1971 (K. M. Knigge, D. E. Scott and A. Weindl, eds.), p. 280–297. Basel: Karger.

  • Wuu, T. C., Crumm, S., Saffran, M.: Amino acid sequence of porcine neurophysin-I. J. biol. Chem. 246, 6043–6063 (1971).

    Google Scholar 

  • Zambrano, D.: Personal Communication. (1971).

  • Zambrano, D., De Robertis, E.: The secretory cycle of supraoptic neurons in the rat. A structural-functional correlation. Z. Zellforsch. 73, 414–431 (1966).

    Google Scholar 

  • Zambrano, D., De Robertis, E.: Ultrastructure of the hypothalamic neurosecretory system of the dog. Z. Zellforsch. 81, 264–282 (1967).

    Google Scholar 

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Supported by U.S.P.H.S. Grant NS 08171.

U.S.P.H.S. Career Development Awardee K04 GM70001.

The authors are deeply indebted to Dr. Finley P. Gibbs and Dr. Sandy Sorrentino, Jr. for their advice and assistance in statistically quantifying autoradiographic data.

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Scott, D.E., Dudley, G.K., Weindl, A. et al. An autoradiographic analysis of hypothalamic magnocellular neurons. Z.Zellforsch 138, 421–437 (1973). https://doi.org/10.1007/BF00307103

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