Summary
Colchicine (0.1 M) or vinblastine (0.01 M) was locally applied on the sciatic nerves of newborn rats. Both colchicine and vinblastine caused reversible disappearance of axonal neurotubules and appearance of increased amounts of neurofilaments at the site of application. Subsequent morphogenesis of myoneural junctions in the tibialis anterior muscle was studied after histochemical demonstration of acetylcholinesterase (AChE; E.C. 3.1.1.7) and non-specific cholinesterase (Ns. ChE; E.C. 3.1.1.8) activity in the myoneural area.
Development of the postsynaptic muscle plasma membrane of the myoneural junction was arrested in the ipsilateral, but not in the contralateral control side, for a period of about three weeks following treatment with the test substances. After this delay the myoneural morphogenesis continued normally and neurotubules were seen in the axoplasm.
Since disruption of neurotubules is likely to cause blockage of the intratubular axoplasmic transport system, it seems possible that the neurotrophic influence responsible for the development of the postsynaptic muscle membrane is mediated through a secretory product transported along axons intratubularly to the nerve endings.
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References
Barondes, S. H.: Further studies of the transport of protein to nerve endings. J. Neurochem. 15, 342–350 (1968).
Dahlström, A.: Effect of colchicine on transport of amine storage granules in sympathetic nerves of rat. Europ. J. Pharmacol. 5, 111–113 (1968).
Dahlström, A.: The effects of drugs of axonal transport of amine storage granules. Bayer-Symposium II, 20–36 (1970).
Dahlström, A.: Effects of vinblastine and colchicine on monoamine containing neurons of the rat, with special regard to the axoplasmic transport of amine granules. Acta neuropath. (Berl.), Suppl. V, 226–237 (1971).
Edström, A., Mattsson, H.: Fast axonal transport in vitro in the sciatic system of the frog. J. Neurochem. 19, 205–214 (1972).
Eränkö, O., Teräväinen, H.: Cholinesterases and eserine-resistant carboxylic esterases in degenerating and regenerating motor end-plates of the rat. J. Neurochem. 14, 947–954 (1967).
Gomori, G.: Microscopic histochemistry, principles and practice, p. 137–221. Chicago: Chicago University Press 1952.
Guth, L.: “Trophic” influences of the nerve on muscle. Physiol. Rev. 48, 4, 645–687 (1968).
Hökfelt, T., Dahlström, A.: Effects of two mitosis inhibitor (colchicine and vinblastine) on the distribution and axonal transport of noradrenaline storage particles, studied by fluorecence and electron microscopy. Z. Zellforsch. 119, 460–482 (1971).
Holtzman, E.: Cytochemical studies of protein transport in the nervous system. Phil. Trans. B. 261, 407–421 (1971).
James, K. A., Bray, J. J., Morgan, I. G., Austin, L.: The effect of colchicine on the transport of axonal protein in the chicken. Biochem. J. 117, 767–771 (1970).
Juntunen, J., Teräväinen, H.: Morphogenesis of myoneural junctions induced postnatally in the tibialis anterior muscle of the rat. Acta physiol. scand. 79, 464–468 (1970).
Juntunen, J., Teräväinen, H.: Structural development of myoneural junctions in the human embryo. Histochemie 32, 107–112 (1972).
Karlsson, J. O., Sjöstrand, J.: The effect of colchicine on the axonal transport of protein in the optic nerve and tract of the rabbit. Brain Res. 13, 617–619 (1969).
Karlsson, J. O., Sjöstrand, J., Hansson, H. A.: Effect of colchicine on axonal transport and morphology of retinal ganglion cells. Z. Zellforsch. 115, 265–283 (1971).
Koelle, G. B.: The elimination of enzymatic diffusion artifacts in the histochemical localization of cholinesterases and a survey of their cellular distributions. J. Pharmacol. exp. Ther. 103, 153–171 (1951).
Korr, I. M., Appeltauer, G.S.I.: Axonal transport of nerve cell proteins to muscle. Fed. Proc. 30, 665 (1971).
Kreutzberg, G. V.: Neuronal dynamics and axonal flow. IV. Blockage of intraaxonal enzyme transport by colchicine. Proc. nat. Acad. Sci. (Wash.) 62, 722–728 (1969).
Malawista, S. E., Bensch, K. G., Sato, H.: Vinblastine and griseofulvine reversibly disrupt the mitotic spindle. Science 160, 770–772 (1968).
Musick, J., Hubbard, J. I.: Release of protein from mouse motor nerve terminals. Nature (Lond) 237, 5353, 297–281 (1972).
Norström, A., Hansson, H-A., Sjöstrand, J.: Effect of colchicine on axonal transport and ultrastructure of the hypothalamo-neurohypophyseal system of the rat. Z. Zellforsch, 113, 271–293 (1971).
Partlow, I. M., Ross, C. D., Motwani, R., McDougal, D. E., Jr.: Transport of axonal enzymes in surviving segments of frog sciatic nerve. J. Gen. Physiol. 60, 388–405 (1972).
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Sabatini, D. D., Bensch, K., Barrnett, R. J.: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17, 19–58, (1963).
Sjöstrand, J., Frizell, M., Hasselgren, P-O.: Effects of colchicine on axonal transport in peripheral nerves. J. Neurochem. 17, 1563–1570 (1970).
Teräväinen, H.: Carboxylic esterase in developing myoneural junctions of rat striated muscle. Histochemie 12, 307–315 (1968 a).
Teräväinen, H.: Development of the myoneural junction in the rat. Z. Zellforsch. 87, 249–265 (1968 b).
Teräväinen, H., Juntunen, J.: Effects of temporary denervation on the development of the acetylcholinesterase-positive structures of the rat myoneural junction. Histochemie 15, 261–269 (1968).
Watson, M. L.: Staining of tissue sections for electron microscopy with heavy metals. J. Cell Biol. 4, 475–478 (1958).
Weiss, P., Hiscoe, H.: Experimentals on the mechanism of nerve growth. J. exp. Zool. 107, 315–396 (1948).
Weiss, P., Hiscoe, H.: Cellular dynamics of the neuron. Cell Biol. (Barondes, S., Ed.) 3–34 (1969).
Wisniewski, H., Shelanski, M. L., Terry, R. D.: Effects of mitotic spindle inhibitors of neurotubules and neurofilaments in anterior horn cells. J. Cell. Biol. 38, 224–229 (1968).
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Juntunen, J. Effects of colchicine and vinblastine on neurotubules of the sciatic nerve and cholinesterases in the developing myoneural junction of the rat. Z.Zellforsch 142, 193–204 (1973). https://doi.org/10.1007/BF00307032
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DOI: https://doi.org/10.1007/BF00307032