The lorenzinian ampullae of Polyodon spathula

  • J. Mørup Jørgensen
  • Å. Flock
  • J. Wersäll
Article

Summary

Light and electron microscopic observations on the ampullary organs of Polyodon spathula (Chondrostei, Osteichthyes) reveal a sensory epithelium similar to that found in the Lorenzinian ampulla, an electroreceptor found in marine Elasmobranchs.

The sensory cells have a very small luminal part provided with a cilium. They are innervated by many nerve endings. Each nerve fibre apparently makes synaptic contact with several sensory cells. The synaptic structure in the sensory cell is composed of a flat sheet, the outermost part of which is surrounded by 3 or 4 annuli of densely staining material. The sheet extends into a protrusion of the sensory cell, and there is a corresponding invagination in the nerve terminal.

The conclusion that these organs are electroreceptors, is supported by the finding that the fish responds to the introduction of an iron tube in the aquarium, whereas a wooden rod introduced in the same way causes no response.

Key-words

Lorenzinian ampullae Polyodon spathula Sensory epithelium Synapses Electron microscopy 

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References

  1. Barets, A., Szabo, T.: Appareil synaptique des cellules sensorielles de l'ampoule de Lorenzini chez la Torpille, Torpedo marmorata. J. Micr. 1, 47–54 (1962).Google Scholar
  2. Barets, A., Szabo, T.: Ultrastructure des cellules sensorielles des mormyromastes de Gnathonemus et de leur appareil synaptique. J. Micr. 3, 85–90 (1964).Google Scholar
  3. Derbin, C., Denizot, J.-P.: Ultrastructure de l'organe ampullaire de Gymnotus carapo (Gymnotidae); origine et nature des mucopolysaccharides. Z. Zellforsch. 113, 531–543 (1971).Google Scholar
  4. Dijkgraaf, S.: The functioning and significance of the lateral-line organs. Biol. Rev. 38, 51–105 (1963).Google Scholar
  5. Dijkgraaf, S.: Biological significance of the lateral line organs. In: Lateral line detectors (P. Cahn, ed.), p. 83–95. Bloomington: Indiana University Press 1967.Google Scholar
  6. Dijkgraaf, S., Kalmijn, A. J.: Verhaltensversuche zur Funktion der Lorenzinischen Ampullen. Naturwissenschaften 49, 400 (1962).Google Scholar
  7. Dijkgraaf, S., Kalmijn, A. J.: Untersuchungen über die Funktion der Lorenzinischen Ampullen an Haifischen. Z. vergl. Physiol. 47, 438–456 (1963).Google Scholar
  8. Dijkgraaf, S., Kalmijn, A. J.: Versuche zur biologischen Bedeutung der Lorenzinischen Ampullen bei den Elasmobranchiern. Z. vergl. Physiol. 53, 187–194 (1966).Google Scholar
  9. Disler, N.: Structure and innervation of the cutaneous sensory organs of the lateral system in Acipenser stellatus. C. R. Acad. URSS. 23, 295–297 (1939).Google Scholar
  10. Dotterweich, H.: Bau und Funktion der Lorenzini'schen Ampullen. Zool. Jb. Allg. Zool. 50, 347–418 (1932).Google Scholar
  11. Engström, H.: The ultrastructure of the sensory cells of the cochlea. J. Laryng. 81, 687–715 (1967).Google Scholar
  12. Flock, Å.: Electron microscopic and electrophysiological studies on the lateral line canal organ. Acta oto-laryng. (Stockh.), Suppl. 199, 1–90 (1965).Google Scholar
  13. Flock, A., Duvall, A. J.: The ultrastructure of the kinocilium of the sensory cells in the inner ear and lateral line organ. J. Cell Biol. 25, 1–8 (1965).Google Scholar
  14. Friedrich-Freksa, H.: Lorenzinische Ampullen bei dem Siluroiden Plotosus anguillaris Bloch. Zool. Anz. 87, 49–66 (1930).Google Scholar
  15. Hamilton, D. W.: The cilium on mammalian vestibular hair cells. Anat. Rec. 164, 253–258 (1969).Google Scholar
  16. Hensel, H.: Die Wirkung thermischer und mechanischer Reize auf die Lorenzinischen Ampullen der Selachier. Pflügers Arch. ges. Physiol. 263, 48–53 (1956).Google Scholar
  17. Hensel, H.: Die Wirkung verschiedener Kohlensäure- und Sauerstoffspannungen auf isolierte Lorenzinische Ampullen von Selachiern. Pflügers Arch. ges. Physiol. 264, 228–244 (1957).Google Scholar
  18. Hoogenboom, K. J. H.: Das Gehirn von Polyodon folium Lacép. Z. mikr.-anat. Forsch. 18, 311–392 (1929).Google Scholar
  19. Ito, S., Karnovsky, M. J.: Formaldehyde-glutaraldehyde fixatives containing trinitro compounds. J. Cell Biol. 39, 168a-169a (1968).Google Scholar
  20. Kistler, H. D.: The primitive pores of Polyodon spathula. J. comp. Neurol. Psych. 16, 294–298 (1906).Google Scholar
  21. Ladman, A. J.: The fine structure of the rod-bipolar cell synapse in the retina of the albino rat. J. biophys. biochem. Cytol. 4, 459–465 (1958).Google Scholar
  22. Leydig, F.: Anatomisch-histologische Untersuchungen über Fische und Reptilien. Berlin: G. Reimer 1853.Google Scholar
  23. Lissmann, H. W.: On the function and evolution of electric organs in fish. J. exp. Biol. 35, 156–191 (1958).Google Scholar
  24. Lissmann, H. W., Machin, K. E.: The mechanism of object location in Gymnarchus niloticus and similar fish. J. exp. Biol. 35, 451–486 (1958).Google Scholar
  25. Lissmann, H. W., Mullinger, A. M.: Organization of ampullary electric receptors in Gymnotidae (Pisces). Proc. roy. Soc. B 169, 345–378 (1968).Google Scholar
  26. Loewenstein, W. R., Ishiko, N.: Sodium chloride sensitivity and electrochemical effects in a Lorenzinian ampulla. Nature (Lond.) 194, 292–294 (1962).Google Scholar
  27. Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 400–414 (1961).Google Scholar
  28. Mullinger, A. M.: The fine structure of ampullary electric receptors in Amiurus. Proc. roy. Soc. B 160, 345–359 (1964).Google Scholar
  29. Mullinger, A. M.: The organization of ampullary sense organs in the electric fish, Gymnarchus niloticus. Tissue & Cell 1, 31–52 (1969).Google Scholar
  30. Murray, R. W.: Evidence for a mechanoreceptive function of the ampullae of Lorenzini. Nature (Lond.) 179, 106–107 (1957).Google Scholar
  31. Murray, R. W.: The response of the ampullae of Lorenzini of elasmobranchs to mechanical stimulation. J. exp. Biol. 37, 417–424 (1960).Google Scholar
  32. Murray, R. W.: The response of the ampullae of Lorenzini of elasmobranchs to electrical stimulation. J. exp. Biol. 39, 119–128 (1962).Google Scholar
  33. Nachtrieb, H. F.: The primitive pores of Polyodon spathula (Walbaum). J. exp. Zool. 9, 455–468 (1910).Google Scholar
  34. Nieuwenhuys, R.: Comparative anatomy of the cerebellum. — Progress in Brain Res. 25, 1–93 (1967).Google Scholar
  35. Norris, H. W.: Observations upon the peripheral distribution of the cranial nerves of certain ganoid fishes (Amia, Lepidosteus, Polyodon, Scaphirhynchus and Acipenser). J. comp. Neurol. 39, 345–416 (1925).Google Scholar
  36. Parker, G. H.: Influence of the eyes, ears, and other allied sense organs on the movements of the dogfish, Mustelus canis (Mitchill). Bull. U.S. Bur. Fish. 29, 43–57 (1909).Google Scholar
  37. Parker, G. H., Heusen, A. P. van: The responses of the catfish, Amiurus nebulosus, to metallic and non-metallic rods. Amer. J. Physiol. 44, 405–420 (1917).Google Scholar
  38. Sand, A.: The function of the ampullae of Lorenzini, with some observations on the effect of temperature on sensory rhythms. Proc. roy. Soc. B 125, 524–553 (1938).Google Scholar
  39. Sjöstrand, F. S.: Ultrastructure of retinal rod synapses of the guinea pig eye as revealed by three-dimensional reconstructions from serial sections. J. Ultrastruct. Res. 2, 122–170 (1958).Google Scholar
  40. Spoendlin, H.: The organization of the cochlear receptor. Fortschr. Hals-Nas.-Ohrenheilk. 13, 1–227 (1966).Google Scholar
  41. Szabo, T.: Sense organs of the lateral line system in some electric fish of the Gymnotidae, Mormyridae and Gymnarchidae. J. Morph. 117, 229–250 (1965).Google Scholar
  42. Szabo, T., Wersäll, J.: Ultrastructure of an electroreceptor (Mormyromast) in a mormyrid fish, Gnathonemus petersii. J. Ultrastruct. Res. 30, 473–490 (1970).Google Scholar
  43. Szamier, R. B., Wachtel, A. W.: Special cutaneous receptor organs of fish: III. The ampullary organs of Eigenmannia, J. Morph. 128, 261–290 (1969).Google Scholar
  44. Szamier, R. B., Wachtel, A. W.: Special cutaneous receptor organs of fish: VI. Ampullary and tuberous organs of Hypopomus. J. Ultrastruct. Res. 30, 450–471 (1970).Google Scholar
  45. Wachtel, A. W., Szamier, R. B.: Special cutaneous receptor organs of fish: The tuberous organs of Eigenmannia. J. Morph. 119, 51–80 (1966).Google Scholar
  46. Wachtel, A. W., Szamier, R. B.: Special cutaneous receptor organs of fish: IV. Ampullary organs of the nonelectric catfish, Kryptopterus. J. Morph. 128, 291–308 (1969).Google Scholar
  47. Waltman, B.: Electrical properties and fine structure of the ampullary canals of Lorenzini. Acta physiol. scand. 66, Suppl. 264, 1–60 (1966).Google Scholar
  48. Wersäll, J.: Studies on the structure and innervation of the sensory epithelium of the cristae ampullares in the guinea pig. Acta oto-laryng. (Stockh.), Suppl. 126, 1–85 (1956).Google Scholar
  49. Wersäll, J., Flock, Å, Lundquist, P. G.: Structural basis for directional sensitivity in cochlear and vestibular sensory receptors. Cold Spr. Harb. Symp. quant. Biol. 30, 115–132 (1965).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • J. Mørup Jørgensen
    • 1
  • Å. Flock
    • 2
    • 3
  • J. Wersäll
    • 2
    • 3
  1. 1.Institute of Comparative AnatomyCopenhagen
  2. 2.King Gustaf Vth Research InstituteStockholm
  3. 3.Dept. of OtolaryngologyKarolinska SjukhusetStockholm

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