Skip to main content
SpringerLink
Log in

Connections between cisternae of the golgi apparatus and the granular endoplasmic reticulum in Amoeba proteus

  • Published:
Zeitschrift für Zellforschung und Mikroskopische Anatomie Aims and scope Submit manuscript

Summary

Numerous morphological continuities between the cisternae of the convex face of the Golgi apparatus and the granular endoplasmic reticulum (GER) were observed in post-division amebae that had divided following enucleation and renucleation. Electron microscopic radioautography with the use of 3H-uridine as a tracer indicated that perhaps the Golgi apparatus is derived from the GER. The possibility of the connections between GER and Golgi apparatus facilitating transport of materials between the two is also discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Caro, L. G., Palade, G. E.: Protein synthesis, storage, and discharge in the pancreatic exocrine cell: An autoradiographic study. J. Cell Biol. 20, 473–495 (1964).

    Google Scholar 

  • Flickinger, C. J.: The effects of enucleation on the cytoplasmic membranes of Amoeba proteus. J. Cell Biol. 37, 300–315 (1968).

    Google Scholar 

  • — The development of Golgi complexes and their dependence upon the nucleus in amebae. J. Cell Biol. 43, 250–262 (1969).

    Google Scholar 

  • Friend, D. S.: The fine structure of Brunner's glands in the mouse. J. Cell Biol. 25, 563–576 (1965).

    Google Scholar 

  • Grimstone, A. V.: Cytoplasmic membrane and the nuclear membrane in the flagellate Trichonympha. J. biophys. biochem. Cytol. 6, 369–378 (1959).

    Google Scholar 

  • Jamieson, J. D., Palade, G. E.: Intracellular transport of secretory proteins in the pancreatic exocrine cell. I. Role of the peripheral elements of the Golgi complex. J. Cell Biol. 34, 577–596 (1967).

    Google Scholar 

  • Jeon, K. W., Lorch, I. J.: New simple method of micrurgy on living cells. Nature (Lond.) 217, 463 (1968).

    Google Scholar 

  • Karnovsky, M. J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use inelectron microscopy. J. Cell Biol. 27, 137A (1965).

    Google Scholar 

  • Kessel, R. G.: Origin of the Golgi apparatus in embryonic cells of the grasshopper. J. Ultrastruct. Res. 34, 260–275 (1971).

    Google Scholar 

  • Meldolesi, J., Jamieson, J. D., Palade, G. E.: Composition of cellular membranes in the pancreas of the guinea pig. II. Lipids. J. Cell Biol. 49, 130–149 (1971).

    Google Scholar 

  • — Composition of cellular membranes in the pancreas of the guinea pig. III. Enzymatic activities. J. Cell Biol. 49, 150–158 (1971).

    Google Scholar 

  • Mollenhauer, H. H.: Transition forms of Golgi apparatus secretion vesicles. J. Ultrastruct. Res. 12, 439–446 (1965).

    Google Scholar 

  • Novikoff, A. B., Shin, W. Y.: The endoplasmic reticulum in the Golgi zone and its relation to microbodies, Golgi apparatus and autophagic vacuoles in rat liver cells. J. Microscopie 3, 187–206 (1964).

    Google Scholar 

  • Prescott, D. M., Carrier, R. F.: Experimental procedure and cultural methods for Euplotes eurystomus and Amoeba proteus. In: Methods in cell physiology (D. M. Prescott, ed.), vol. I, p. 85–95. New York: Academic Press 1965.

    Google Scholar 

  • Sjöstrand, F. S., Hanzon, V.: The ultrastructure of the Golgi-apparatus of the exocrine cells of the mouse pancreas. Exp. Cell Res. 7, 415–429 (1954).

    Google Scholar 

  • Stevens, A. R.: High resolution autoradiography. In: Methods in cell physiology (D. M. Prescott, ed.), vol. II, p. 255–310. New York: Academic Press 1966.

    Google Scholar 

  • Wise, G. E., Flickinger, C. J.: Relation of the Golgi apparatus to the cell coat in amoebae. Exp. Cell Res. 61, 13–23 (1970).

    Google Scholar 

  • — Cytochemical staining of the Golgi apparatus in Amoeba proteus. J. Cell Biol. 46, 620–626 (1970).

    Google Scholar 

  • Wolstenholme, D. R., Plaut, W. J.: Cytoplasmic DNA-containing bodies in Amoeba proteus. III. Further studies on the nature of the DNA-containing elements. J. Cell Biol. 22, 505–513 (1964).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado

    Gary E. Wise

Authors
  1. Gary E. Wise
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

The author is indebted to Dr. Lester Goldstein for generously supplying the transplanted nuclei and for reviewing the manuscript. Thanks are also extended to Mary Beeson and Ellen Codner for assistance in culturing and labeling the Tetrahymena and amebae.

This research was supported by U.S. Public Health Service research grant GM 15156 to Dr. Lester Goldstein.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wise, G.E. Connections between cisternae of the golgi apparatus and the granular endoplasmic reticulum in Amoeba proteus . Z.Zellforsch 126, 431–436 (1972). https://doi.org/10.1007/BF00306903

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00306903

Key words

Search

Navigation