Summary
The feeding tentacles of Choanophrya contain a central canal lined by microtubules. Only one tentacle develops during metamorphosis of the embryo into the adult, but others develop at intervals throughout adult life. Each tentacle forms adjacent to a solitary, subcortical kinetosome which lies parallel to the body surface, lacks accessory elements and never develops a cilium. Small condensations of electron-dense material and short bundles of microtubules form adjacent to the cartwheel region of the kinetosome. Initially these bundles are orientated randomly but later they become radially arranged and curved into prolamellae around a disc-shaped condensation centre, to form a paddlewheel-like tentacle primordium 0.8–1.1 μm in diameter. The condensation centre consists of alternating concentric electron-dense and electron-transparent zones, and lies with its axis perpendicular to both the kinetosome and the cortex. The microtubules in each prolamella increase in number and pairs of short tip microtubules develop between adjacent prolamellae. Subsequently the developing lamellae become enclosed by a cylinder of ring microtubules. Once all the microtubule components of the tentacle primordium are established it increases in length by addition of material to the basal ends of the microtubules to form a short microtubule canal. As the canal elongates the epiplasm above it disappears and the pellicle membranes become uplifted around the protruding tentacle. An epiplasmic collar differentiates around the growing tentacle whilst spheroid vesicles and solenocysts begin to accumulate in the surrounding cytoplasm.
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References
Allen, T.D.: A cytological study of Dendrocometes paradoxus Stein. Ph. D. Thesis, University of Manchester (1970)
Anderson, R.G.W., Brenner, R.M.: The formation of basal bodies (centrioles) in the rhesus monkey oviduct. J. Cell Biol. 50, 10–34 (1971)
Bardele, C.F.: Budding and metamorphosis in Acineta tuberosa. An electron microscopic study on morphogenesis in Suctoria. J. Protozool. 17, 51–70 (1970)
Bardele, C.F.: Microtubule model system: Cytoplasmic transport in the suctorian tentacle and the centrohelidan axopod. In: 29th Annual EMSA Meeting (ed. C.J. Arconeaux), p. 334–335. Baton Rouge: Claitor's Publishing Division 1971
Batisse, A.: Quelques données sur la permanence des cinétosomes durant la phase adulte des Acinétiens. C. R. Acad. Sci. (Paris) 266, 130–132 (1968)
Brown, D.L., Bouck, G.B.: Microtubule biogenesis and cell shape in Ochromonas. II. The role of nucleating sites in shape development. J. Cell Biol. 56, 360–378 (1973)
Cachon, J., Cachon, M.: Le système axopodial des Radiolaires sphaeroïdés. I. Centroaxoplastidiés. Arch. Protistenk. 114, 51–64 (1972)
Dippell, R.V.: The development of basal bodies in Paramecium. Proc. nat. Acad. Sci. (Wash.) 61, 461–468 (1968)
Fulton, C., Dingle, A.D.: Basal bodies, but not centrioles, in Naegleria. J. Cell Biol. 51, 826–836 (1971)
Grimstone, A.V.: Structure and formation of some fibrillar organelles in Protozoa. In: Formation and fate of cell organelles (ed. Warren, K.B.), p. 219–232. New York: Academic Press 1967
Hartog, M.M.: Notes on Suctoria. Arch. Protistenk. 1, 372–374 (1901)
Hascall, G.K., Rudzinska, M.A.: Metamorphosis in Tokophrya infusionum; an electronmicroscope study. J. Protozool. 17, 311–323 (1970)
Hauser, M.: Elektronenmikroskopische Untersuchung an dem Suktor Paracineta limbata Maupas. Z. Zellforsch. 106, 584–614 (1970)
Heller, G.: Elektronenmikroskopische Untersuchung zur Bildung und Struktur von Conoid, Rhoptrien, und Mikronemen bei Eimeria steidae (Sporozoa, Coccidia). Protistologica 8, 43–51 (1972)
Hitchen, E.T.: A cytological study of Choanophrya infundibulifera Hartog and Rhyncheta cyclopum Zenker. Ph. D. Thesis, University of Manchester (1972)
Hitchen, E.T., Butler, R.D.: Tentacle morphogenesis in Choanophrya infundibulifera Hartog (Ciliata, Suctorida). J. Protozool. 19 (Suppl.) 135 (1972a)
Hitchen, E.T., Butler, R.D.: A redescription of Rhyncheta cyclopum Zenker (Ciliatea, Suctorida). J. Protozool. 19, 597–601 (1972b)
Hitchen, E.T., Butler, R.D.: Ultrastructural studies of the commensal suctorian, Choanophrya infundibulifera Hartog. I. Tentacle structure, movement and feeding. Z. Zellforsch. 144, 37–57 (1973)
McIntosh, J.R., Ogata, E.S., Landis, S.C.: The axostyle of Saccinobaculus I. Structure of the organism and its microtubule bundle. J. Cell Biol. 56, 304–323 (1973)
Mignot, J.P., Puytorac, P. de: Sur la structure et la formation du style chez l'Acinétien Discophrya piriformis Guilcher. C. R. Acad. Sci. (Paris) 266, D 593–595 (1968)
Millecchia, L.L., Rudzinska, M.A.: Basal body replication and ciliogenesis in a suctorian Tokophrya infusionum. J. Cell Biol. 46, 553–563 (1970a)
Millecchia, L.L., Rudzinska, M.A.: The ultrastructure of brood pouch formation in Tokophrya infusionum. J. Protozool. 17, 574–583 (1970b)
Millecchia, L.L., Rudzinska, M.A.: The permanence of the infraciliature in Suctoria: An electronmicroscopic study of pattern formation in Tokophrya infusionum. J. Protozool. 19, 473–483 (1972)
Novikoff, A.B., Holtzman, E.: Cells and organelles. New York: Holt, Reinhart and Winston 1970
Pickett-Heaps, J.: The autonomy of the centriole: Fact or fallacy? Cytobios. 3, 205–214 (1971)
Pitelka, D.R.: Centriole replication. In: Handbook of molecular cytology (ed. Lima-de-Faria, A.), p. 1199–1218. Amsterdam-London: North Holland Publish. Co 1969
Szollosi, D.A.: Morphological changes in mouse eggs due to aging in the Fallopian tube. Amer. J. Anat. 130, 209–226 (1971)
Szollosi, D., Calarco, P., Donahue, R.P.: Absence of centrioles in the first and second meiotic spindles of mouse oocytes. J. Cell Sci. 11, 521–541 (1972)
Tilney, L.G.: How microtubule patterns are generated. The relative importance of nucleation and bridging of microtubules in the formation of the axoneme of Raphidiophrys. J. Cell Biol. 51, 837–854 (1971)
Tilney, L.G., Goddard, J.: Nucleating sites for the assembly of cytoplasmic microtubules in the ectodermal cells of blastulae of Arbacia punctulata. J. Cell Biol. 46, 564–575 (1970)
Tucker, J.B.: Morphogenesis of a large microtubular organelle and its association with basal bodies in the ciliate Nassula. J. Cell Sci. 6, 385–429 (1970)
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This investigation was supported by the J.S. Dunkerley Fellowship in Protozoology, awarded by the University of Manchester.
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Hitchen, E.T., Butler, R.D. Ultrastructural studies of the commensal suctorian, Choanophrya infundibulifera hartog. Z.Zellforsch 144, 59–73 (1973). https://doi.org/10.1007/BF00306686
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DOI: https://doi.org/10.1007/BF00306686