Summary
A cytoplasmic inclusion comparable to nucleoluslike bodies in other neurons has been observed in cells of the nucleus dorsalis vagi lateralis of the rat medulla oblongata. This body which lacks a limiting membrane is composed of filaments 20 to 70 Å thick and granules 60 to 150 Å in diameter. The filamentous elements attach to the surrounding ribosomes. The cytoplasmic bodies are either large and fairly compact or small with the appearance of aggregated dense flecks. Furthermore a double-membrane bounded body near the nucleus, and sporadic accumulations of filaments and granules similar to those composing the nucleoluslike, bodies are also reported. Enzyme digestion tests reveal that nucleoluslike bodies contain mainly protein and lack detectable ribonucleic acid. The possible origin of these bodies is discussed.
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Bak, I. J., Hassler, R.: Electron microscopical observations on the cytoplasmic nucleolar extrusion bodies in substantia nigra in mouse. In: Macromolecules and the function of the neuron (Z. Lodin and S. P. R. Rose eds.). Amsterdam: Excerpta Medica Foundation. 1968.
Bernhard, W., Granboulan, N.: Electron microscopy of the nucleolus in vertebrate cells. In: The nucleus (A. J. Dalton and F. Haguenau eds.). New York and London: Academic Press. 1968.
Bernhard, W.: A new staining procedure for electron microscopical cytology. J. Ultrastruct. Res. 27, 250–265 (1969).
Eddy, E. M., Ito, S.: Fine structural and radioautographic observations on dense perinuclear cytoplasmic material in tadpole oocytes. J. Cell Biol. 49, 90–108 (1971).
Gambetti, P., Gonatas, N. K., Flexner, L. B.: The fine structure of puromycin-induced changes in mouse entorhinal cortex. J. Cell Biol. 36, 379–390 (1968).
Huxley, H. E., Zubay, G.: Preferential staining of nucleic acid-containing structures for electron microscopy. J. biophys. biochem. Cytol. 11, 273–296 (1961).
Karlsson, U.: Three-dimensional studies of neurons in the lateral geniculate nucleus of the rat. I. Organelle organization in the perikaryon and its proximal branches. J. Ultrastruct. Res. 16, 429–481 (1966).
Kawabata, I.: Electron microscopy of the rat hypothalamic neurosecretory system. II. Nucleolus-like inclusion bodies in the cytoplasm of neurosecretory cells. Arch. histol. jap. 26, 101–113 (1965).
Korfsmeier, K. H.: Ultrastrukturelle Veränderungen in den neurosekretorischen Zentren des Hypothalamus und in der Eminentia mediana nach Behandlung mit Cyproteronazetat (Antiandrogen). Z. Zellforsch. 110, 600–610 (1970).
Le Beux, Y. J.: An ultrastructural study of the neurosecretory cells of the medial vascular prechiasmatic gland, the preoptic recess and the anterior part of the suprachiasmatic area. I. Cytoplasmic inclusions resembling nucleoli. Z. Zellforsch. 114, 404–440 (1971).
Le Beux, Y. J., Langlier, P., Poirier, L. J.: Further ultrastructural data on the cytoplasmic nucleolus resembling bodies or nematosomes. Their relationship with the subsynaptic web and a cytoplasmic filamentous network. Z. Zellforsch. 118, 147–155 (1971).
Leduc, E. H., Bernhard, W.: Recent modificatiosn of the glycol methacrylate embedding procedure. J. Ultrastruct. Res. 19, 196–199 (1967).
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).
Millonig, G.: A modified procedure for lead staining of thin sections. J. biophys. biochem. Cytol. 11, 736–739 (1961).
Palay, S. L., McGee-Russell, S. M., Gordon, S., Grillo, M. A.: Fixation of neural tissues for electron microscopy by perfusion with solutions of osmium tetroxide. J. Cell Biol. 12, 385–410 (1962).
Palay, S. L., Sotelo, C., Peters, A., Orkand, P. M.: The axon hillock and the initial segment. J. Cell Biol. 38, 193–201 (1968).
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–213 (1963).
Rosenbluth, J.: The fine structure of acoustic ganglia in the rat. J. Cell Biol. 12, 329–359 (1962).
Rosenbluth, J.: Redundant myelin sheaths and other ultrastructural features of the toad cerebellum. J. Cell Biol. 28, 73–93 (1966).
Sano, M., Sotokawa, Y.: A possible mechanism for the nucleolar extrusion in nerve cells of the mouse hypothalamus. Arch. histol. jap. 33, 199–208 (1971).
Schoefl, G. I.: The effect of actinomycin D on the fine structure of the nucleolus. J. Ultrastruct. Res. 10, 224–243 (1964).
Shima, R.: Zur vergleichenden Anatomie des dorsalen Vaguskerns. Arb. neurol. Inst. Univ. Wien 17, 190–216 (1908).
Shimizu, N., Ishii, S.: Electron-microscopic observations on nucleolar extrusion in nerve cells of the rat hypothalamus. Z. Zellforsch. 67, 367–372 (1965).
Swift, H.: Cytochemical studies on nuclear fine structure. Exp. Cell Res., Suppl. 9, 54–67 (1963).
Wartenberg, H., Baumgarten, H. G.: Untersuchungen zur fluorescenz- und elektronenmikroskopischen Darstellung von 5-Hydroxytryptamin (5-HT) im Pineal-Organ von Lacerta viridis und L. muralis. Z. Anat. Entwickl.-Gesch. 128, 185–210 (1969).
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The author is indebted to Prof. H. Yamada and Associate Prof. K. Kohno for their guidance and valuable advice in this study.
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Kishi, K. Fine structural and cytochemical observations on cytoplasmic nucleoluslike bodies in nerve cells of rat medulla oblongata. Z.Zellforsch 132, 523–532 (1972). https://doi.org/10.1007/BF00306639
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DOI: https://doi.org/10.1007/BF00306639