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Die Differenzierungsgenese der TSH-Zellen von Xenopus laevis unter Normalbedingungen und nach Thiouracilbehandlung

Differentiation of TSH-cells in Xenopus laevis under normal conditions and after thiouracil-treatment

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Summary

The ultrastructural changes of the TSH-cells in Xenopus laevis have been investigated in the course of normal development and after thiouracil-treatment. Individually at stage 45, and in larger numbers at stage 47, the thyrotropic cells are detected first in young larvae by their polymorph granulation. Significant changes in size and shape of these secretory granules occur during the different developmental and functional periods. The size-minimum at stage 49 K (early discharge period) is followed by an enlargement of the granules and a rounding-off of their shape up to stage 59 K (period of storage). Up to stage 61 K a decrease in granule-size as well as the appearance of a new population of small, more polymorph elements are to be observed (discharge and new formation).

After MTU-treatment a corresponding course of granule-modifications is to be found, but if compared with the controls, there are larger granule-sizes as well as variations regarding age and stage of the animals. The granulation and other cytologic criteria confirm and complete current ideas of the functional development of the pituitary-thyroid-system during the ontogeny of the Amphibia.

Zusammenfassung

Die Strukturveränderungen der TSH-Zellen von Xenopus laevis im Verlaufe der Normalentwicklung und nach Belastung mit Methylthiouracil sind elektronenmikroskopisch untersucht worden. Folgende Ergebnisse wurden erzielt: Die thyreotropen Zellen lassen sich aufgrund ihrer polymorphen Granulation erstmals bei jungen Larven vereinzelt im Stadium 45, zahlreicher im Stadium 47, nachweisen. Form und Größe der sekretorischen Granula zeigen in verschiedenen Entwicklungs- und Funktionsphasen auffällige Veränderungen. Nach einem Durchmesserminimum im Stadium 49 K (frühe Ausschüttungsphase) erfolget eine Granulavergrößerung und Abrundung der Granulaform bis Stadium 59 K (Speicherphase). Bis Stadium 61 K ist eine Granulaverkleinerung und das Auftreten einer neuen, kleinen, stärker polymorphen Granulapopulation beobachtet worden (Ausschüttung und Neubildung).

Nach MTU-Behandlung zeigt sich ein entsprechender Verlauf der Granulaveränderungen, jedoch mit höheren Durchschnittswerten und zeitlich wie stadienbezogenen Verschiebungen gegenüber den Kontrollen. Die funktionelle Deutung der Veränderungen des Granulastatus sowie die übrigen cytologischen Kriterien bestätigen und ergänzen unsere Vorstellungen vom Aktivitätsverlauf des Hypophysen-Schilddrüsen-Systems in der Amphibienontogenese.

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  1. Anatomisches Institut der Universität Kiel, Germany

    L. Hemme

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Herrn Univ.-Doz. Dr. F.-W. Pehlemann danke ich für die Anregung zu dieser Arbeit, für Anleitung und jederzeit hilfreiche Beratung, Herrn Prof. Dr. med. W. Bargmann für die Überlassung eines Arbeitsplatzes.

Mit dankenswerter Unterstützung durch die Deutsche Forschungsgemeinschaft.

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Hemme, L. Die Differenzierungsgenese der TSH-Zellen von Xenopus laevis unter Normalbedingungen und nach Thiouracilbehandlung. Z.Zellforsch 125, 353–377 (1972). https://doi.org/10.1007/BF00306632

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