Summary
The cell cycle time and the doubling time of mouse spleen cells producing 19S hemolytic antibody against sheep red blood cells were determined in vitro.
The doubling time of the number of hemolytic plaque forming cells was found to be 4–7 hours. By a combination of the hemolytic plaque assay and the double labeling method with 3H- and 14C-thymidine the duration of the S-period and the cell cycle time were determined to be 8–9 hours and 13–15 hours, respectively.
The disagreement in doubling time and cycle time of PFC is discussed and a possible explanation by cell recruitment is presented.
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Herrn Prof. Dr. W. Maurer zum 65. Geburtstag gewidmet.
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Lang, W. Determination of S-period and cell cycle time of 19S hemolysin producing spleen cells of mice cultured in vitro . Z.Zellforsch 125, 332–338 (1972). https://doi.org/10.1007/BF00306630
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DOI: https://doi.org/10.1007/BF00306630