Summary
The intestinal caeca of the intraredial cercaria of Neophasis lageniformis differ in several aspects from previous descriptions of the intestinal caeca of other digenetic trematodes. The caeca are lined with a single-layered, non-syncytial epithelium with lamellate projections into the lumen. The cells are rich in glycogen particles and contain abundant granular endoplasmic reticulum. The apical cell membrane, which displays a unit membrane structure, is always covered by an external membrane of the same thickness as a unit membrane but showing no double-layered substructure. The external membrane, which is not equivalent to a glycocalyx, may continue from one cell to another. Apically on the projections the external membranes are apparently pushed out forming large, extracellular whorls, which fill up the caecal lumen. In the lumen there are also found crystal-like structures and lipid-like bodies which are suspended in a granular substance. The possible origin and function of the extracellular bodies are discussed.
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Aaronson, S., Behrens, U., Orner, R., Haines, T. H.: Ultrastructure of intracellular and extracellular vesicles, membranes, and myelin figures produced by Ochromonas danica. J. Ultrastruct. Res. 35, 418–430 (1971).
Bogitsh, B. J.: Cytochemical and biochemical observations on the digestive tract of digenetic trematodes. IX. Megalodiscus temperatus. Exp. Parasit. 32, 244–266 (1972).
Bogitsh, B. J., Davis, D. A., Nunnally, D. A.: Cytochemical and biochemical observations on the digestive tracts of digenetic trematodes. II. Ultrastructural localization of acid phosphatase in Haematoloechus medioplexus. Exp. Parasit. 23, 303–308 (1968).
Bogitsh, B. J., Shannon, W. A.: Haematoloechus medioplexus: Enzymatic oxidation of 3,3′-diaminobenzidine in mitochondria. Exp. Parasit. 28, 186–193 (1970).
Bogitsh, B. J., Shannon, W. A.: Cytochemical and biochemical observations on the digestive tracts of digenetic trematodes. VIII. Acid phosphatase activity in Schistosoma mansoni and Schistosomatium douthitti. Exp. Parasit. 29, 337–347 (1971).
Bruce, J. I., Pezzlo, F., Yajima, Y., McCarty, J. E.: An electron microscopic study of Schistosoma mansoni migration through mouse tissue: ultrastructure of the gut during the hepatoportal phase of migration. Exp. Parasit. 30, 165–173 (1971).
Davis, D. A., Bogitsh, B. J.: Arylsulfatase activity in Gorgoderina attenuata and Haematolocheus medioplexus: Cytochemical and biochemical observations on the digestive tracts of digenetic trematodes. Exp. Parasit. 29, 302–308 (1971a).
Davis, D. A., Bogitsh, B. J.: Gorgoderina attenuata: Cytochemical and biochemical observations on the digestive tracts of digenetic trematodes. Exp. Parasit. 29, 320–329 (1971b).
Davis, D. A., Bogitsh, B. J., Nunnally, D. A.: Cytochemical and biochemical observations on the digestive tracts of digenetic trematodes. I. Ultrastructure of Haematoloechus medioplexus gut. Exp. Parasit. 22, 96–106 (1968).
Davis, D. A., Bogitsh, B. J., Nunnally, D. A.: Cytochemical and biochemical observations on the digestive tracts of digenetic trematodes. III. Nonspecific esterase in Haematoloechus medioplexus. Exp. Parasit. 24, 121–129 (1969).
Dike, S. C.: Ultrastructure of the caeca of the digenetic trematodes Gorgodera amplicava and Haematoloechus medioplexus. J. Parasit. 53, 1173–1185 (1967).
Dike, S. C.: Acid phosphatase activity and ferritin incorporation in the caeca of digenetic trematodes. J. Parasit. 55, 111–123 (1969).
Ebrahimzadeh, A., Kraft, M.: Ultrastrukturelle Untersuchungen zur Anatomie der Cercarien von Schistosoma mansoni. I. Der Verdauungskanal. Z. Parasitk. 32, 157–175 (1969).
Gallagher, S. S. E., Threadgold, L. T.: Electron-microscope studies of Fasciola hepatica. II. The interrelationship of the parenchyma with other organ systems. Parasitology 57, 627–632 (1967).
Gresson, R. A. R., Threadgold, L. T.: A light and electron microscope study of the epithelial cells of the gut of Fasciola hepatica L. J. biophys. biochem. Cytol. 6, 157–167 (1959).
Halton, D. W.: Occurrence of microvilli-like structures in the gut of digenetic trematodes. Experientia (Basel) 22, 828–829 (1966).
Ito, S.: Structure and function of the glycocalyx. Fed. Proc. 28, 12–25 (1969).
Køie, M.: On the histochemistry and ultrastructure of the redia of Neophasis lageniformis (Lebour, 1910) (Trematoda, Acanthocolpidae). Ophelia 9, 113–143 (1971a).
Køie, M.: On the histochemistry and ultrastructure of the tegument and associated structures of the cercaria of Zoogonoides viviparus in the first intermediate host. Ophelia 9, 165–206 (1971b).
Køie, M.: The host-parasite interface and associated structures of the cercariae and adult Neophasis lageniformis (Lebour, 1910) (Trematoda, Acanthocolpidae) studied by scanning and transmission electron-microscopy. Ophelia 12 (in press) (1973).
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).
Marshall, A. T., Cheung, W. W.: Ultrastructure and cytochemistry of an extensive plexiform surface coat on the midgut cells of a fulgorid insect. J. Ultrastruct. Res. 33, 161–172 (1970).
Morris, G. P.: Fine structure of the gut epithelium of Schistosoma mansoni. Experientia (Basel) 24, 480–482 (1968).
Rothman, A. H.: Enzyme localization and colloid transport in Haematoloechus medioplexus. J. Parasit. 54, 286–294 (1968).
Rothman, A. H., Elder, J. E.: Histochemical nature of an acanthocephalan, a cestode and a trematode absorbing surface. Comp. Biochem. Physiol. 33, 745–762 (1970).
Senda, H., Wake, K., Hayano, T., Nakai, K., Jo, S., Fujiwara, T., Iwamoto, T., Mori, K.: On the myelinoid bodies, developed in microvilli of jejunal epithelium incubated in an isotonic aquous solution of CCl4. Electron Microscopy. R. Uyeda, ed. vol. II, p. 395–396. Tokyo: Maruzen Co. Ltd. 1966.
Senft, A. W., Philpott, D. E., Pelofsky, A. H.: Electron microscope observations of the integument, flame cells and gut of Schistosoma mansoni. J. Parasit. 47, 217–229 (1961).
Shannon, W. A., Bogitsh, B. J.: Cytochemical and biochemical observations on the digestive tracts of digenetic trematodes. V. Ultrastructure of Schistosomatium douthitti gut. Exp. Parasit. 26, 344–353 (1969).
Spence, I. M., Silk, M. H.: Ultrastructural studies of the blood fluke—Schistosoma mansoni. IV. The digestive system. S. Afr. J. med. Sci. 35, 93–112 (1970).
Thorsell, W., Björkman, N.: Morphological and biochemical studies on absorption and secretion in the alimentary tract of Fasciola hepatica. J. Parasit. 51, 217–223 (1965).
Threadgold, L. T.: Electron microscope studies of Fasciola hepatica. VI. The ultrastructural localization of phosphatases. Exp. Parasit. 23, 264–276 (1968).
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The author wishes to thank Prof. Dr.C. Overgaard Nielsen and Dr. K. J. Pedersen, Inst ute of General Zoology, Copenhagen, for use of the electron microscope facilities.
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Køie, M. The ultrastructure of the caecal epithelium of the intraredial cercaria of Neophasis lageniformis (Lebour, 1910) (Trematoda, Acanthocolpidae). Z.Zellforsch 139, 405–416 (1973). https://doi.org/10.1007/BF00306594
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DOI: https://doi.org/10.1007/BF00306594