Skip to main content
Log in

Liquid formaldehyde in catecholamine studies

A new approach to the morphological localization of monoamines in the adrenal medulla and the supraoptic nucleus of the rat

  • Published:
Histochemie Aims and scope Submit manuscript

Summary

Based on the fluorescence technique for demonstration of catecholamines a method has been elaborated using liquid, 100% formaldehyde and embedding in Epon. This method has been applied for a comparative light-, fluorescence- and electron microscopical study of the adrenal medulla. In the supraoptic nucleus this fluorescence technique as well as conventional electron microscopy have been used for the localization of synaptic terminals.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Corrodi, H., N.-Å. Hillarp, and G. Jonsson: Fluorescence methods for the histochemical demonstration of monoamines. 3. Sodium borohydride reduction of the fluorescent compounds as a specificity test. J. Histochem. Cytochem. 12, 582–586 (1964).

    Google Scholar 

  • —, and G. Jonsson: Fluorescence methods for the histochemical demonstration of monoamines. 4. Histochemical differentiation between dopamine and noradrenaline in models. J. Histochem. Cytochem. 13, 484–487 (1965).

    Google Scholar 

  • Csillik, B., and F. Joó: Cholinesterase, arylesterase and the structural basis of neurohumoral transmission in the central nervous system. Acta biol. Acad. Sci. hung. 16, 185–205 (1965).

    Google Scholar 

  • De Robertis, E.: Contribution of electronmicroscopy to some neuropharmacologioal problems. Biochem. Pharmacol. 9, 49–59 (1962).

    Google Scholar 

  • —, and A. Pellegrino de Iraldi: A plurivesicular component in adrenergic nerve endings. Anat. Rec. 139, 299 (1961).

    Google Scholar 

  • Eneström, S., and A. Hamberger: Morphology and respiratory activity of the supraoptic nucleus neurons in the rat. A correlative study in osmotic stress. To be published.

  • -, and Chr. Svalander: Freeze-drying for electronmicroscopy using the moving-gas principle. To be published.

  • Fuxe, K.: Evidence for the existence of monoamine neurons in the central nervous system. III. The monoamine nerve terminal. Z. Zellforsch. 65, 573–596 (1965).

    Google Scholar 

  • —, T. Hökfelt, and O. Nilsson: A fluorescence and electronmicroscopic study on certain brain regions rich in monoamine terminals. Amer. J. Anat. 117, 33–38 (1965).

    Google Scholar 

  • Hökfelt, T.: A modification of the histochemical fluorescence method for the demonstration of catecholamines and 5-hydroxytryptamine, using araldite as embedding medium. J. Histochem. Cytochem. 13, 518–520 (1965).

    Google Scholar 

  • —: Electron microscopic observations on nerve terminals in the intrinsic muscles of the albino rat iris. Acta physiol. scand. 67, 255–256 (1966).

    Google Scholar 

  • Malmfors, T.: Studies on adrenergic nerves. Acta physiol. scand. 60, Suppl. 248, 1–93 (1965).

    Google Scholar 

  • Matsui, T., and H. Kobayashi: Histochemical demonstration of monoamine oxidase in the hypothalamo-hypophysial system in the tree sparrow and the rat. Z. Zellforsch. 68, 172–182 (1965).

    Google Scholar 

  • Moline, S. W., and G. G. Glenner: Ultrarapid tissue freezing in liquid nitrogen. J. Histochem. Cytochem. 12, 777–783 (1964).

    Google Scholar 

  • Pellegrino de Iraldi, A., H. Farini Duggan, and E. de Robertis: Adrenergic synaptic vesicles in the anterior hypothalamus of the rat. Anat. Rec. 145, 521–531 (1963).

    Google Scholar 

  • Shimizu, N., and S. Ishii: Electron microscopic histochemistry of acetylcholinesterase of rat brain by Karnovsky's method. Histochemie 6, 24–33 (1966).

    Google Scholar 

  • Tramezzani, J. H., S. Chiocchio, and G. F. Wassermann: A technique for light and electron microscopic identification of adrenalin- and noradrenalin-storing cells. J. Histochem. Cytochem. 12, 890–899 (1964).

    Google Scholar 

  • Walker, J. F.: Some properties of anhydrous formaldehyde. J. Amer. chem. Soc. 55, 2821–2825 (1933).

    Google Scholar 

  • —: Formaldehyde, 3rd ed. New York: Reinhold Publ. Corp. 1964.

    Google Scholar 

  • Watson, M. L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4, 475–478 (1958).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Eneström, S., Svalander, C. Liquid formaldehyde in catecholamine studies. Histochemie 8, 155–163 (1967). https://doi.org/10.1007/BF00306468

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00306468

Keywords

Navigation