Skip to main content
SpringerLink
Log in

Verteilung und Lokalisation induzierter Brüche auf den Chromosomen von Bellevalia

III. Natriumnitrit

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Nitrous acid induces fragmentation of chromosomes and only very few translocations in Bellevalia romana (2n=8). A total of 1098 breaks is induced by 1×10-3, 1,5×10-4 and 1,5×10-5 M NaNO2 at pH 4,5. Root tips were fixed at 24, 48 and 72 hours after treatment for 1 hour.

The percentage of breaks increases with time between treatment and fixation; it reaches its highest level at 1,5×10-4 M, which dose coincides with the lowest mitotic rate.

A dose influence on the distribution of breaks among the centromeres and the chromosome arms was observed. Consistent with the effect of other agents the highest number of breaks was in the A- the lowest, however, in the D-chromosome.

The distribution of breaks on the different chromosome arms was homogeneous for all fixations. As observed previously the breakage frequency was not proportional to the arm length.

The distribution of breaks on the proximal, median and distal sections of the chromosome arms is significantly different from random distribution.

The nitrous acid-induced pattern of breaks is significantly different from that induced by methylating (MMS+NMU) and ethylating agents (DES+NEU).

The known primary chemical reaction of nitrous acid and alkylating agents with DNA are inadequate alone in explaining the different patterns of breaks. More information is needed regarding the processes following these primary reactions.

In conclusion, several possible objections against the technique of localizing breaks are shown to be non-valid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  • Becker, E.F., B.K. Zimmermann, and E.P. Geiduschek: Structure and function of cross linked DNA. I. Reversible denaturation and Bacillus subtilis transformation. J. molec. Biol. 8, 377–391 (1964).

    Google Scholar 

  • Gläss, E.: Nicht zufallsgemäße Verteilung röntgeninduzierter Brüche auf den Chromosomen von Bellevalia romana. Port. Acta biol. A 8, 153–176 (1964).

    Google Scholar 

  • —: Die Anordnung der Chromosomen und die röntgeninduzierten Chromatidtranslokationen bei Bellevalia romana. Int. J. Radiat. Biol. 11, 145–151 (1966a).

    Google Scholar 

  • —: Verteilung und Lokalisation induzierter Brüche auf den Chromosomen von Bellevalia I. Methylphenylnitrosamin, Diäthylsulfat, Methylmethansulfonat. Z. Vererbungsl 98, 167–179 (1966b).

    Google Scholar 

  • —: Verteilung und Lokalisation induzierter Brüche auf den Chromosomen von Bellevalia. II. N-nitroso-N-methylurethan und N-nitroso-N-äthylurethan. Z. Vererbungsl. 98, 363–374 (1966c)

    Google Scholar 

  • Kohn, K.W. and D. M. Green: Transforming activity of nitrogen mustard-crosslinked DNA. J. molec. Biol. 19, 289–302 (1966).

    Google Scholar 

  • —: Inter-strand crosslinking of DNA by nitrogen-mustard. J. molec. Biol. 19, 266–288 (1966).

    Google Scholar 

  • —: Cross linking and repair of DNA in sensitive and resistant strains of E. coli treated with nitrogen-mustard Proc. nat. Acad. Sci. (Wash.) 53, 1154–1161 (1965).

    Google Scholar 

  • Lochmann, E.R., u. W. Stein: Die Wirkung von Natriumnitrit auf Nukleinsäurebasen und ihr Verhältnis zur Inaktivierung von Hefezellen. Z. Naturforsch. 18b, 809–816 (1963).

    Google Scholar 

  • Loveless, A.: Genetic and allied effects of alkylating agents. London 1966.

  • Marquardt, H., U. von Laer u. F.K. Zimmermann: Das spontane Nitrosamid- und Nitritinduzierte Mutationsmuster von 6 Adenin-Genloci der Hefe. Z. Vererbungsl. 98, 1–9 (1966).

    Google Scholar 

  • —: Nitrosamide als mutagene Agentien. Naturwissenschaften 50, 625 (1963).

    Google Scholar 

  • —: Die Wirkung krebsauslösender Nitrosamine und Nitrosamide auf das Adenin 6-45-Rückmutationssystem von Saccharomyces cerevisiae. Z. Vererbungsl. 95, 82–96 (1964).

    Google Scholar 

  • Nashed, N., and G. Jabbur: A genetic and functional characterization of adenine mutants induced in yeast by 1-nitroso-imidazolidone-2 and nitrous acid. Z. Vererbungsl 98, 106–110 (1966).

    Google Scholar 

  • Schuster, H.: Die Reaktionsweise der Desoxyribonucleinsäure mit salpetriger Säure. Z. Naturforsch. 15b, 298–304 (1960).

    Google Scholar 

  • Schwaier, R.: Vergleichende Mutationsversuche mit sieben Nitrosamiden im Rückmutationstest an Hefen. Z. Vererbungsl. 97, 55–67 (1965).

    Google Scholar 

  • —: Chemical constitution and mutagenic efficiency: mutation induction in Saccharomyces cerevisiae by a homologous series of N-nitroso-N-methylcarbonamides. Z. Vererbungsl. 98, 309–319 (1966a).

    Google Scholar 

  • —, F.K. Zimmermann, and U. von Laer: The effect of temperature on the mutation induction in yeast by N-alkylnitrosamides and nitrous acid. Z. Vererbungsl 97, 72–74 (1965a.

    Google Scholar 

  • Serres, F.J. de: Mutagenesis and chromosome structure. J. cell. comp. Physiol. 64, Suppl. 1, 33–42 (1964).

    Google Scholar 

  • Strauss, B.S.: The presence of breaks in the deoxyribonucleic acid of Bacillus subtilis treated in vivo with the alkylating agent methylmethansulfonate. Biochim. biophys. Acta (Amst.) 80, 116–126 (1964).

    Google Scholar 

  • Zimmermann, F.K., H. Marquardt, and R. Schwaier: Comparative studies on the mutagenic action of carcinogenic substances, nitrous acid and diethylsulfate in Saccharomyces cerevisiae. Proc. XI. Int. Congr. of Genetics, The Hague, The Netherlands, Sept. 1963. In: S.J. Geerts (ed.) Genetics today vol. I, p. 59. New York: Pergamon Press 1963.

    Google Scholar 

  • —: Eine ungewöhnliche Dosiswirkungs-Beziehung der N-nitroso-N-Methylacetamid induzierten Mutationsraten bei Saccharomyces cerevisiae. Z. Vererbungsl 94, 261–268 (1963).

    Google Scholar 

  • —: The influence of pH on the mutagenicity in yeast of N-methylnitrosamides and nitrous acid. Z. Vererbungsl. 97, 68–71 (1965).

    Google Scholar 

  • —: The effect of temperature on the mutation fixation in yeast. Mutation Res. 3, 90–92 (1966a).

    Google Scholar 

  • —: The effect of residual growth on the frequency of reverse mutations induced with nitrous acid and 1-nitroso-imidazolidone-2 in yeast Mutation Res. 3, 171–173 (1966b).

    Google Scholar 

  • —: Nitrous acid and alkylating nitrosamides: mutation fixation in Saccharomyces cerevisiae. Z. Verebungsl. 98, 152–166 (1966c).

    Google Scholar 

  • —: Mitotic recombination induced in Saccharomyces cerevisiae with nitrous acid, diethylsulfate and carcinogenic, alkylating nitrosamides. Z. Vererbungsl. 98, 230–246 (1966d).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Forstbotanisches Institut der Universität Freiburg i. Br., Frei burg i. Br, Deutschland

    E. Gläss & H. Marquardt

Authors
  1. E. Gläss
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Marquardt
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gläss, E., Marquardt, H. Verteilung und Lokalisation induzierter Brüche auf den Chromosomen von Bellevalia . Molec. Gen. Genetics 99, 40–48 (1967). https://doi.org/10.1007/BF00306456

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00306456

Search

Navigation