Zusammenfassung
Es wird beim Phagen T4Bo reine Schutzwirkung von Cysteamin gegenüber den nach Einbau von 5-Bromuracil in die DNS biologisch zusätzlich beobachtbaren UV-Strahlenschäden beschrieben. Bei BU-Phagen, nicht aber bei normalen Phagen und auch nicht bei BU-Phagen bestrahlt in Anwesenheit von 10-2 m Cysteamin, wird eine Zerstörung von Desoxyribose in der DNS gemessen. Der Mechanismus, der zum Abbau von DNS-Pentose führt bzw. die Reaktionen, die in Anwesenheit eines Radikalfängers den Schutzeffekt bewirken, werden im Rahmen einer Arbeitshypothese diskutiert. Da bei den T-Phagen die Gruppe der näher untersuchten enzymatischen Reaktivierungsmechanismen, nämlich Photo-, Wirtszell- und u-Gen-Reaktivierung, hinsichtlich des BU-Phänomens gemeinsame Merkmale zeigt, d. h. Blockierung durch BU und Aufhebung des Blocks bei Bestrahlung in Anwesenheit von Cysteamin, liegt die Annahme nahe, daß Schäden an der Desoxyribose der DNS-Helix für die Blockierung der enzymatischen Reaktivierungsschritte verantwortlich sind.
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References
Adams, M. H.: Methods of study of bacterial viruses. In: J. H. Comroe, Methods in medical research, vol. 2, p. 1. Chicago: Year Book Publ. 1950.
Boyce, R. P., and P. Howard-Flanders: Genetic control of DNA breakdown and repair in E. coli K 12 treated with mitomycin C or ultraviolet light. Z. Vererbungsl. 95, 345 (1964).
Dische, Z.: Color reactions of nucleic acid components. In: E. Chargaff and I. N. Davidson, The nucleic acids, vol. 1, p. 285. New York: Academic Press 1955.
Hanawalt, P. C., and R. H. Haynes: Repair replication of DNA in bacteria: Irrelevance of chemical nature of base defect. Biochem. biophys. Res. Commun. 19, 462 (1965).
Harm, W.: Zur Deutung der unterschiedlichen UV-Empfindlichkeit der Phagen T2 und T4. Naturwissenschaften 45, 391 (1958).
—: Host-cell reactivation of non-lethal ultraviolet effects. Photochem. Photobiol. 4, 575 (1965).
Hotz, G.: Suppression by cysteamine of radiosensitization in 5-bromodeoxyuridine substituted phage T1. Biochem. biophys. Res. Commun. 11, 393 (1963).
—: Photoreactivation of uv-damage in phage containing 5-bromouracil-DNA. Z. Vererbungsl. 95, 211 (1964).
Hotz, G.: Unpublished results 1966.
—: Experiments in radiation chemistry of T1-phage. Int. J. Radiat. Biol. 7, 75 (1963).
Ishihara, H., and S. Y. Wang: Photochemistry of 5-bromouracils: Isolation of 5,5′-diuracils. Nature (Lond.) 210, 1222 (1966).
Koehnlein, W., and F. Hutchinson: ESR studies of normal and bromouracil substituted transforming DNA of Bacillus subtilis after irradiation with ultraviolet light. 3rd Int. Congr. Radiat. Res., Cortina d'Ampezzo 1966 (Abstract).
Lion, M. B.: UV-Photoproducts in 5-bromouracil substituted DNA of Escherichia coli 15 T −. Radiat. Res. 25, 211 (1965) (Abstract).
Papirmeister, B., and C. L. Davidson: Elimination of sulfur mustard-induced products from DNA of E. coli. Biochem. biophys. Res. Commun. 17, 608 (1964).
Rubinstein, I., C. A. Thomas jr., and A. D. Hershey: The molecular weights of T2-bacteriophage DNA and its first and second breakage products. Proc. nat. Acad. Sci. (Wash.) 47, 1113 (1961).
Rupert, C. S.: Questions regarding the presumed role of thymine dimer in photoreactivable u.v. damage to DNA. Photochem. Photobiol. 3, 399 (1964).
Rupp, W. D., and W. H. Prusoff: Photochemistry of iodouracil. II. Effects of sulfur compounds, ethanol and oxygen. Biochem. biophys. Res. Commun. 18, 158 (1965).
Sauerbier, W.: The influence of 5-bromodeoxyuridine substitution on uv-sensitivity, host-cell reactivation, and photoreactivation in T1 and P22 H5. Virology 15, 465 (1961).
—: Host cell reactivation of gamma rayed T1. Biochem. biophys. Res. Commun. 17, 46 (1964a).
—: Search for repair of ultraviolet damage in mutants of bacteriophage T4. Biochim. biophys. Acta (Amst.) 91, 663 (1964b).
Setlow, R. B., and R. Boyce: The action spectra for ultraviolet light inactivation of systems containing 5-bromouracil-substituted deoxyribonucleic acid. II. Bacteriophage T4. Biochim. biophys. Acta (Amst.) 68, 455 (1963).
—: The disappearance of thymine dimers from DNA: An error correcting mechanism. Proc. nat. Acad. Sci. (Wash.) 51, 226 (1964).
Smith, K.: The photochemistry of thymine and bromouracil in vivo. Photochem. Photobiol. 3, 1 (1964).
Stahl, F. W., J. M. Craseman, L. Okun, E. Fox, and C. Laird: Radiation sensitivity of bacteriophage containing 5-bromodeoxyuridine. Virology 13, 98 (1961).
Streisinger, G.: The genetic control of uv sensitivity levels in bacteriophages T2 and T4. Virology 2, 1 (1956).
Takahashi, I., and J. Marmur: Deplacement of thymidylic acid by deoxyuridylic acid in the deoxyribonucleic acid of a transducing phage for Bacillus subtilis. Nature (Lond.) 197, 794 (1963).
Wacker, A.: Molecular mechanisms of radiation effects. In: J. N. Davidson and W. E. Cohn, Progress in nucleic acid research, vol. 1. New York: Academic Press 1963.
Wulff, D. L., and C. S. Rupert: Disappearance of thymine photodimer in ultraviolet irradiated DNA upon treatment with a photoreactivating enzyme from baker's yeast. Biochem. biophys. Res. Commun. 7, 237 (1962).
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Hotz, G., Reuschl, H. Damage to deoxyribose molecules and to U-gene reactivation in UV-irradiated 5-bromouracil-DNA of phage T4 Bo ras influenced by cysteamine. Molec. Gen. Genetics 99, 5–11 (1967). https://doi.org/10.1007/BF00306453
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DOI: https://doi.org/10.1007/BF00306453