Summary
The Authors proved, in the course of a general investigation about the Alcian blue staining of cartilage, that the specificity of this dye is due to its inability of staining in the presence of a proteic fraction, which is present in a greater amount in the non-glycid structures containing ionized groups at 2,4 pH: i.e. collagen, DNA etc.
This protein is easily soluble in various salt solutions and in 1 M sulphuric acid, but insoluble in 3% acetic acid. Removal of this protein by these agents or by peptic digestions result in increasing the mucopolysaccharides staining, but in decreasing or suppressing electivity.
Differents abnormalities of Alcian method are explained by the Authors in this way.
Resumé
Les Auteurs, à la suite d'une serie des récherches sur la coloration au bleu Alcian de la cartilage, on pu démontrer que la signaléticité de ce colorant basique envers les mucopolysaccharides est due à la interférence inhibitive avec une fraction protéique présente en grande quantité dans les structures non-mucopolysaccharidiques ionisées au pH de utilisation (2,4) (DNA, collagéne etc.), fraction bien soluble dans les solutions salines et dans l'acide sulphurique 1 M, mais peu soluble dans l'acide acétique 3%.
L'asportation de cette substance à l'aide de dits solvants ou de la digestion à la pepsine, augmente la colorabilité des mucopolysaccharides, mais diminue ou abolit la signaléticité.
Sur cette donné, les Auteurs expliquent les differentes anomalies de la méthode aux Alcians.
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Palladini, G., Lauro, G. Observations sur la significativité de la coloration aux Alcians pour les mucopolysaccharides. Histochemie 16, 15–22 (1968). https://doi.org/10.1007/BF00306208
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DOI: https://doi.org/10.1007/BF00306208