Summary
65Zn was infused at a constant rate for 10 days into a rat. Glutaraldehyde fixed, Epon-araldite embedded ultrathin sections of pancreatic tissue were coated with Ilford L4 emulsion and at 211 days exposure were developed. Silver grains were found over the zymogen granules and over the rough endoplasmic reticulum of exocrine cells. Islet tissue was not observed in these studies. The failure of other zinc localization methods to demonstrate zinc in acinar tissue is discussed as are some of the pitfalls of the autoradiographic method and suggestions for future improvement.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amsterdam, A., and M. Schramm: Rapid release of the zymogen granule protein by osmium tetroxide and its retention during fixation by glutaraldehyde. J. Cell Biol. 29, 199–207 (1966).
Dockum, N. L.: Autoradiographic localization of Zn65 (review method), U.S. Atomic Energy Commission Hanford Biology Research Annual Report for 1961, p. 97–101.
Euler, C. von: On the significance of the high zinc content in the hippocampal formation. Physiol. de l'hippocampe, Colloques internat. Centre National Recherche Scientifique, No 107, 135–145 (1962).
Folk, J. E., K. A. Piez, W. R. Carroll, and J. A. Gladner: Carboxypeptidase B. IV. Purification and characterization of the porcine enzyme. J. biol. Chem. 235, 2272–2277 (1960).
Gorycki, M. A.: Staining and washing ultrathin sections; a device for handling various materials simultaneously. Stain Technol. 41, 169–172 (1966).
Jamieson, J. D.: Intracellular transport of secretory proteins in the pancreatic exocrine cell. II. Transport to condensing vacuoles and zymogen granules. J. Cell Biol. 34, 597–615 (1967).
—, and G. E. Palade: I. Role of the peripheral elements of the Golgi complex. J. Cell Biol. 34, 577–596 (1967).
Keilin, D., and T. Mann: Carbonic anhydrase. Nature (Lond.) 144, 442 (1939).
Mager, M., W. R. McNary, Jr., and F. Lionetti: The histochemical detection of zinc. J. Histochem. Cytochem. 1, 493–504 (1953).
Maske, H.: Interaction between insulin and zinc in the islets of Langerhans. Diabetes 6, 335–341 (1957).
—, H. Wolff, B. Stampfl u. F. Baumgarten: Beobachtungen über den Zinkstoffwechsel beim Alloxandiabetes (ein Beitrag zur Alloxanwirkung). Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 216, 457–472 (1952).
Maunsbach, A. B.: Absorption of I125-labeled homologous albumin by rat kidney proximal tubule cells. J. Ultrastruct. Res. 15, 197–241 (1966).
McIsaac, R. J.: Distribution of Zinc-65 in the rat pancreas. Endocrinology 57, 571–579 (1955).
McNary, W. F., Jr.: Zinc-dithizone reaction of pancreatic islets. J. Histochem. Cytochem. 2, 185–193 (1954).
Millar, M. J., N. R. Vincent, and C. A. Mawson: An autoradiographic study of the distribution of Zinc-65 in rat tissues. J. Histochem. Cytochem. 9, 111–116 (1961).
Mills, C. F., J. Quarterman, R. B. Williams, A. C. Dalgarno, and B. Panić: The effects of zinc deficiency on pancreatic carboxypeptidase activity and protein digestion and absorption in the rat. Biochem. J. 102, 712–718 (1967).
Mollenhauer, H. H.: Plastic embedding mixtures for use in electron microscopy. Stain Technol. 39, 111–114 (1964).
Montgomery, M. L., G. E. Sheline, and I. L. Chaikoff: The elimination of administered zinc in the pancreatic juice, duodenal juice, and bile of the dog as measured by its radioactive isotope (Zn65). J. exp. Med. 78, 151–159 (1943).
Müller, D., u. H. Güthert: Untersuchungen mit der Sulfidsilbermethode über das Zink in den Langerhansschen Inseln des syrischen Goldhamsters. Virchows Arch. path. Anat. 333, 97–100 (1960).
Okamoto, K.: Biologische Untersuchungen der Metalle. VI. Histochemischer Nachweis einiger Metalle in den Geweben, besonders in den Nieren, und deren Veränderungen. Trans. Soc. path. Jap. 32, 99–105 (1942).
—: VII. Über das Gewebseisen der Malarialeber und Milz, die Zinkverteilung im Tierreich und den Zinkstoffwechsel. Trans. Soc. path. Jap. 33, 247–252 (1943).
—: Experimental studies on the pathogenesis of diabetes mellitus. Folia endocr. Jap. 25, 32–61 (1949).
—: Experimental pathology of diabetes mellitus II. Tohoko J. exp. Med. (Suppl. III) 61, 1–116 (1955).
—, and H. Kawanishi: Submicroscopic histochemical demonstration of intracellular reactive zinc in beta cells of pancreatic islets. Endocr. Jap. 13, 305–318 (1966).
Otsuka, N., u. M. Kawamoto: Histochemische und autoradiographische Untersuchungen der Hippocampusformation der Maus. Histochemie 6, 267–273 (1966).
Pihl, E.: Ultrastructural localization of heavy metals by a modified sulfide-silver method. Histochemie 10, 126–139 (1967).
—, and S. Falkmer: Trials to modify the sulfide-silver method for ultrastructural tissue localization of heavy metals. Acta histochem. (Jena) 27, 34–41 (1967).
Runge, W., I. Müller u. H. Ferner: Der Zinknachweis in den A-Zellen und B-Zellen des Inselorganes bei der Ente. Z. Zellforsch. 44, 208 (1956).
Schmidt, R., u. R. Rautschke: Der Zink- und Kupfergehalt verschiedener Organe der weißen Ratte; ein Beitrag zur Dithizon-Zink-Reaktion in der Histochemie. Acta histochem. (Jena) 19, 1–13 (1964).
Shearer, T. R., and J. W. Suttie: Effect of fluoride administration on plasma fluoride and food intake in the rat. Amer. J. Physiol. 212, 1165–1168 (1967).
Stampfl, B.: Das Zink in den Langerhansschen Inseln, seine Verteilung und sein Verhalten beim experimentellen Diabetes. Verh. dtsch. Ges. Path. (Freiburg) 36, 321–324 (1952).
—: Die intravitale histochemische Darstellung des Zink durch Dithizon. Acta histochem. (Jena) 8, 406–447 (1959).
Timm, F.: Zur Histochemie der Schwermetalle. Das Sulfid-Silberverfahren. Dtsch. Z. ges. gerichtl. Med. 46, 706–711 (1958).
Vallee, B. L., and H. Neurath: Carboxypeptidase, a zinc metalloenzyme. J. biol. Chem. 217, 253–261 (1955).
Voigt, G. E.: Untersuchungen mit der Sulfidsilbermethode an menschlichen und tierischen Bauchspeicheldrüsen (unter besonderer Berücksichtigung des Diabetes mellitus und experimenteller Metallvergiftungen). Virchows Arch. path. Anat. 332, 295–323 (1959).
Wetterdal, B.: Experimental studies on radioactive zinc in the male reproductive organs of the rat. Acta radiol. (Stockh.), Suppl. 156, 1–83 (1958).
Wolff, H., H. Maske, B. Stampfl u. F. Baumgarten: Untersuchungen über den Dithizondiabetes. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 216, 440–446 (1952).
—, u. D. Ringleb: Histochemische Untersuchungen über das Inselzink. Z. ges. exp. Med. 124, 236–256 (1954).
Wolff, H. P., D. Ringleb u. R. Amann: Histochemische Untersuchungen über das Inselzink (II. Histophotometrische Messungen). Z. ges. exp. Med. 126, 390–416 (1955).
Author information
Authors and Affiliations
Additional information
Published with the approval of the Director of the Wisconsin Agricultural Experiment Station, Madison. Supported in part by USPHS Research Grant AM-05606 from the Nat. Institute of Arthritis and Metabolic Diseases.
Supported by an NIH post-doctoral fellowship.
Rights and permissions
About this article
Cite this article
Westmoreland, N., Hoekstra, W.G. Ultrastructural localization of zinc in rat exocrine pancreas by autoradiography. Histochemie 18, 261–266 (1969). https://doi.org/10.1007/BF00306172
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00306172