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Histochemical method for the demonstration of the activity of phosphoglucomutase

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Summary

A method for the demonstration of the activity of phosphoglucomutase in tissues is described. In the histochemical system the enzyme converts the substrate α-D-glucose-1-phosphate to α-D-glucose-6-phosphate. The resulting α-D-glucose-6-phosphate is oxidized by exogenous glucose-6-phosphate dehydrogenase to D-glucono-δ-lactone-6-phosphate, whereby the activity of endogenous NADPH-tetrazolium oxidoreductase reduces Nitro-BT to the slightly soluble diformazan. The problems involved in the histochemical demonstration of phosphoglucomutase are discussed.

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Authors and Affiliations

  1. Department of Pathological Anatomy, Biochemical Section, University of Amsterdam, The Netherlands

    A. E. F. H. Meijer

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  1. A. E. F. H. Meijer
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Meijer, A.E.F.H. Histochemical method for the demonstration of the activity of phosphoglucomutase. Histochemie 8, 248–251 (1967). https://doi.org/10.1007/BF00306087

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  • DOI: https://doi.org/10.1007/BF00306087

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