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Identification and characterization of DNA clones encoding group-II glycinin subunits

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Summary

DNA clones that encode the group-II subunits of soybean glycinin were identified and compared with clones for group-I subunits. The group-I clones hybridize weakly to those from group-II at low stringency, but fail to hybridize with them at moderate or high stringency. The genes for the group-II subunits are contained in 13 and 9 kb EcoRI fragments of genomic DNA in cultivar CX635-1-1-1. These fragments contain genes for subunits A5A4B3 and A3B4, respectively. The larger size of mature group-II subunits compared with group-I subunits is correlated with a larger sized mRNA. However, the gross arrangement of introns and exons within the group-II coding regions appears to be the same as for the genes which encode group-I subunits. Messenger RNA for both groups of glycinin subunits appear in the seed at the same developmental interval, and their appearance lags slightly behind that of mRNAs for the a/a′ subunits of β-conglycinin. These data indicate that the glycinin gene family is more complex than previously thought.

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Abbreviations

bp:

base pairs

kb:

kilobase pairs

SDS:

sodium dodecyl sulfate

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Authors and Affiliations

  1. USDA and the Department of Agronomy, Purdue University, 47907, West Lafayette, IN, USA

    B. Scallon, V. H. Thanh & L. A. Floener

  2. ARS and the Department of Agronomy, Purdue University, 47907, West Lafayette, IN, USA

    N. C. Nielsen

Authors
  1. B. Scallon
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  2. V. H. Thanh
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  3. L. A. Floener
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  4. N. C. Nielsen
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Additional information

Communicated by D. von Wettstein

Cooperative research between USDA/ARS and the Indiana Agric. Expt. Station. This work was supported in part by grants from the USDA Competitive Grants Program and the American Soybean Association Research Foundation. This is Journal Paper No. 10,078 from the Purdue Agricultural Experiment Station

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Scallon, B., Thanh, V.H., Floener, L.A. et al. Identification and characterization of DNA clones encoding group-II glycinin subunits. Theoret. Appl. Genetics 70, 510–519 (1985). https://doi.org/10.1007/BF00305984

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  • DOI: https://doi.org/10.1007/BF00305984

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