Summary
The carcinogenic p-Dimethylaminoazobenzene and the non-carcinogenic p-aminoazobenzene were radioactive labelled and applicated to Whistar rats intraperitoneally. A very significant difference in binding tendencies for the two compounds and their metabolites was observed in rat liver tissues, rat liver nuclei and rat liver chromatin. Above all a specific binding affinity of the carcinogen to nuclear non-histone protein was found.
Zusammenfassung
Nach radioaktiver Markierung wurden das krebserzeugende p-Dimethylaminoazobenzol und das inaktive p-Aminoazobenzol intraperitoneal an Whistarratten appliziert. Es ergaben sich starke Unterschiede in der zeitabhängigen Verteilung, wobei das Carcinogen und seine Metaboliten eine sehr viel höhere Bindungsfähigkeit zu Rattenleberzellkernen und — chromatin zeigt als das nichtcarcinogene Analogon. Schließlich konnte eine signifikante Bindung von p-Dimethylaminoazobenzol an Nichthistonproteine des Zellkerns festgestellt werden.
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Herrn Prof. Dr. A. Butenandt zum 75. Geburtstag gewidmet.
Diese Arbeit wurde begonnen, als die Abteilung noch von Herrn Prof. Dr. H. Dannenberg (†) geleitet wurde. Wir gedenken seiner großbzügigen Förderung in Dankbarkeit.
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Sonnenbichler, J., Reichhart, F. Wechselwirkung von p-Dimethylaminoazobenzol mit Nichthistoprotein aus Rattenlebercliromatin. Z. Krebsforsch. 91, 55–61 (1978). https://doi.org/10.1007/BF00305972
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DOI: https://doi.org/10.1007/BF00305972