Summary
Rats bearing Guerin epithelioma were exposed to 3 or 6 sessions of local microwave hyperthermia (LMwH) (2450 MHz, tumor temperature 43° C, time of exposition 45 min) on the 3rd or 5th week after implantation of tumor cells. In some of the animals LMwH was combined with the administration of immunopotentiating (IP) substances—purified Streptolysin S (SLS) (1 mg/kg b.w.) or Corynebacterium parvum (CP) (1 mg/kg b.w., intratumorally)-injected 3 times weekly on the 4th and 6th weeks after implantation of tumor cells. All the animals were immunized with bovine serum albumine (BSA) 2 and 3 weeks after tumor implantation. Size of tumors was measured each 4 days and the growth curves were analysed. Eight weeks after implantation all the animals were killed and the following tests were performed: weight of tumors, BSA antibodies titre (passive hemagglutination test using BSA-glutaraldehyde coupled sheep red blood cells), 3H-leucine and 3H-uridine incorporation to phytohemagglutinin (PHA)-stimulated spleen lymphocytes and to mixed tumor cell-lymphocyte cultures, cytotoxicity of isolated spleen lymphocytes and peritoneal macrophages to 51Cr-labeled primary cultures of Guerin tumor cells, lysozyme (muramidase) activity in blood serum.
LMwH inhibited growth of Guerin tumors and the effect was enhanced by combined treatment with IP substances. Combined application of LMwH and CP resulted in complete disappearance of 9 of 12 tumors, while LMwH and IP applied separately led to eradication of 2–5 tumors of 12.
LMwH stimulated reactivity of the T-lymphocyte and macrophage systems enhancing both non-specific (increased production of BSA antibodies, high reactivity of spleen lymphocytes to PHA, increased serum lysozyme levels) and specific (increased cytotoxicity of lymphocytes and macrophages to cultured tumor cells and higher reactivity of lymphocytes to mitomycin-inhibited tumor cells) immune responses.
The role of immunostimulation by LMwH is stressed as an important factor in tumor-inhibiting effect of hyperthermia in addition to the well known selective heat killing of neoplastic cells.
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This work was supported by research grants 05-339-C from the US Public Health Service and PR-6-210-02 from the Polish Governmental Program
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Szmigielski, S., Janiak, M., Hryniewicz, W. et al. Local microwave hyperthermia (43° C) and stimulation of the macrophage and T-lymphocyte systems in treatment of Guerin epithelioma in rats. Z. Krebsforsch. 91, 35–48 (1978). https://doi.org/10.1007/BF00305970
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DOI: https://doi.org/10.1007/BF00305970