Summary
A previously described electron histochemical method for detecting cystine in fully keratinised human hair has been modified for the examination of this amino acid residue amongst the cellular components of anagen guinea pig hair follicles. Using the technique the progressive incorporation of cystine into the hair cuticle and cortex has been observed. Although cystine was absent from the hair medulla and outer root sheath cells at all stages of development, a narrow layer of cystine-containing material was found adjacent to the cell membranes of hardened inner root sheath cells.
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Barrnett, R. J., and R. F. Sognnaes: Histochemical distribution of protein-bound sulfhydryl and disulphide groups in vertebrate keratins. In: Fundamentals of Keratinisation, edit. E. O. Butcher and R. F. Sognnaes, publ. no. 70 of the American Association of the Advancement of Science, p. 27–43 (1962).
Mercer, E. H.: Keratin and Keratinisation. New York: Pergamon Press 1961.
Rambourg, A., and C. P. Leblond: Electron microscope observations on the carbohydrate-rich cell coat present at the surface of cells in the rat. J. Cell Biol. 32, 27–53 (1967).
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208 (1963).
Richards, F. M., and J. R. Knowles: Glutaraldehyde as a protein cross-linked reagent. J. molec. Biol. 37, 231–233 (1968).
Sabatini, D. D., K. Bensch, and R. J. Barrnett: Cytochemistry and electron microscopy — The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17, 19–58 (1963).
Swift, J. A.: The electron histochemistry of cystine-containing proteins in thin transverse sections of human hair. J. roy. micr. Soc. 88, 449–460 (1968).
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Swift, J.A. The electron histochemical demonstration of cystine-containing proteins in the guinea pig hair follicle. Histochemie 19, 88–98 (1969). https://doi.org/10.1007/BF00305965
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DOI: https://doi.org/10.1007/BF00305965