Summary
The effects of anhydrous benzoylation were examined in connection with the following protein end-group methods: diazosulfanilic acid — Azure A, the Sakaguchi reaction and a fluorescent variant, the phenanthrequinone fluorescent method for arginine, the Millon reaction, the Morel-Sisley reaction, the mercury orange method, the alloxan and ninhydrinShiff methods, the Dansyl-chloride fluorescent method for lysine, the dimethylaminobenz-aldehydenitrite (DMAB) method for tryptophan, and the acid fluorochrome brilliant sulfoflavin used at pH 2.8 as a stain for basic groups. With all of these methods except the DMAB method for tryptophan, selective blockage of cytoplasmic proteins and staining of nuclei was obtained. With the DMAB reaction, the results were reversed: cytoplasmic staining exceeded nuclear staining after benzoylation.
This is a preview of subscription content, log in via an institution to check access.
References
Adams, C. W. M.: Ap-dimethylaminobenzaldehyde-nitrite method for the histochemical demonstration of tryptophane and related compounds. J. clin. Path. 10, 56–62 (1957).
Bachmann, R., Seitz, H. M.: Zur histochemischen Darstellung des Histidin mit Diazoniumsalzen. Histochemie 2, 307–312 (1961).
Barnard, E. A.: Acylation and diazonium coupling in protein cytochemistry with special reference to the benzoylation-tetrazonium method. In: General cytochemical methods (J. F. Danielli, ed.), vol. 2, p. 203–258. New York: Academic Press 1961.
—, Bell, L. G. E.: Selective cytochemical demonstration of arginine groups in nucleoprotein in the cell nucleus. Nature (Lond.) 187, 508–509 (1960).
—, Danielli, J. F.: A cytochemical reaction for nucleoprotein. Nature (Lond.) 178, 1450–1453 (1956).
Bennett, H. S., Watts, R. M.: The cytochemical demonstration and measurement of sulfhydryl groups by azo-aryl-mercaptide coupling, with special reference to mercury orange. In: General cytochemical methods (J. F. Danielli, ed.), vol. 1, p. 317–374. New York: Academic Press 1958.
Deitch, A. D.: Microspectrophotometric study of the binding of the anionic dye, naphthol yellow S by tissue sections and by purified proteins. Lab. Invest. 4, 324–251 (1955).
Lillie, R. D.: Histopathologic technic and practical histochemistry, 3rd ed. New York: McGraw-Hill 1965.
—, Palmer, R. W., Gutierrez, A.: A diazosulfanilic a acid azure A sequence for tissue protein. J. Histochem. Cytochem. 14, 421–424 (1966).
Maddy, A. H.: 1-Fmoro-2∶4-dinitrobenzene as a cytochemical reagent. In: General cytochemical methods (J. F. Danielli, ed.), vol. 2, p. 259–285. New York: Academic Press (1961).
Magun, B. E., Kelly, J. W.: A new fluorescent method with phenanthrenequinone for the histochemioal demonstration of arginine residues in tissues. J. Histochem. Cytochem. 17, 821–827 (1969).
Notenboom, C. D., Veerdonk, F. C. G. van de, Kamer, J. C. van de: A fluorescent modification of the Sakaguchi reaction on arginine. Histochemie 9, 117–121 (1967).
Pearse, A. G. E.: Histochemistry, theoretical and applied, 2nd ed. London: Churchill 1960.
Rasch, E., Swift, H.: Microspectrophotometric analysis of the cytochemical Millon reaction. J. Histochem. Cytochem. 8, 4–17 (1960).
Ritter, C., Berman, J.: The quantitative spectrophotometric analysis of tyrosine by a modified diazotization-coupling method. J. Histochem. Cytochem. 11, 590–602 (1963).
Rosselet, A., Ruch, F.: Cytofluorometric determination of lysine with dansylchloride. J. Histochem. Cytochem. 16, 459–466 (1968).
Yasuma, A., Ichikawa, T.: Ninhydrin-Schiff and alloxan-Schiff staining. A new histochemical staining method for protein. J. Lab. clin. Med. 41, 296–299 (1953).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Curtis, S.K., Cowden, R.R. Effects of anhydrous benzoylation on staining with a variety of protein end-group methods. Histochemie 23, 185–188 (1970). https://doi.org/10.1007/BF00305852
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00305852