Skip to main content
Log in

Karyotype analysis of regenerated plants from callus cultures of interspecific hybrids of cultivated barley (Hordeum vulgare L.)

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

The karyotype of 82 regenerated plants from callus cultures of interspecific hybrids between cultivated barley (Hordeum vulgare L.) and seven polyploid wild barley species was examined by C-banding or Feulgen staining. The karyotypic changes observed in 46 plants included aneuploidy, double haploidy, amphidiploidy, deletions, inversions, extra C-bands, and extra euchromatic segments. Apparently, chromosome 5, 6, and 7 of H. vulgare were more frequently exposed to elimination or structural change than the other chromosomes of this species. Irradiation of calli seemed to enhance the occurrence of karyotypic variants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Barbier M, Dulieu HL (1980) Effects génétiques observés sur des plantes de tabac régénérées á partir de cotylédons par culture in vitro. Ann Amel Plant 30:321–344

    Google Scholar 

  • Bothmer R von, Hagberg A (1983) Pre-breeding and wide hybridization in barley. Genetika, Ser F, Suppl III:41–53

  • Bothmer R von, Jacobsen N (1986) Interspecific crosses in Hordeum (Poaceae). Plant Syst Evol 153:49–64

    Google Scholar 

  • Bothmer R von, Flink J, Jacobsen N, Kotimäki M, Landström T (1983) Interspecific hybridization with cultivated barley (H. vulgare L.). Hereditas 99:219–244

    Google Scholar 

  • Döbel P, Schubert I, Rieger R (1978) Distribution of heterochromatin in a reconstructed karyotype of Vicia faba as identified by banding - and DNA-late replication patterns. Chromosoma 69:193–209

    Google Scholar 

  • Finch RA (1983) Tissue-specific elimination of alternative whole parental genomes in one barley hybrid. Chromosoma 88:386–393

    Google Scholar 

  • Gaponenko AK, Petrova TF, Iskakov AR, Sozinov AA (1988) Cytogenetics of in vitro cultured somatic cells and regenerated plants of barley (Hordeum vulgare L.). Theor Appl Genet 75:905–911

    Google Scholar 

  • Hagberg A (1966) Induced structural mutations in barley, especially translocation, and their use in further directed production of variation. In: Mutations in plant breeding. IAEA, Vienna, pp 109–116

    Google Scholar 

  • Jensen CJ (1977) Monoploid production by chromosome elimination. In: Reinert J, Bajaj YPS (eds) Fundamental aspects of plant cell, tissue and organ culture. Springer, Berlin Heidelberg New York pp 299–331

    Google Scholar 

  • Jessop CM, Subrahmanyam NC (1984) Nucleolar number variation in Hordeum species, their haploids and interspecific hybrids. Genetica 64:93–100

    Google Scholar 

  • Johnson SS, Phillips RL, Rines HW (1987) Possible role of heterochromatin in chromosome breakage induced by tissue culture in oats (Avena sativa L.). Genome 29:439–446

    Google Scholar 

  • Jørgensen RB (1986) Relationships in the barley genes (Hordeum): An electrophoretic examination of proteins. Hereditas 104:273–291

    Google Scholar 

  • Jørgensen RB, Jensen CJ, Andersen B, Bothmer R von (1986) High capacity of plant regeneration from callus of interspecific hybrids with cultivated barley (Hordeum vulgare L.). Plant Cell, Tissue Organ Cult 6:199–207

    Google Scholar 

  • Karp A, Bright SWJ (1985) On the causes and origins of somaclonal variation. Oxford Surveys Plant Mole Cell Biol 2:199–234

    Google Scholar 

  • Karp A, Steele SH, Parmar S, Jones MGK, Shewry PR, Breiman A (1987) Relative stability among barley plants regenerated from cultured immature embryos. Genome 29:405–412

    Google Scholar 

  • Lapitan NLV, Sears RG, Gill BS (1984) Translocations and other karyotypic structural changes in wheat × rye hybrids regenerated from tissue culture. Theor Appl Genet 68:547–554

    Google Scholar 

  • Larkin PJ, Scowcroft WR (1981) Somaclonal variation - a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214

    Google Scholar 

  • Linde-Laursen I (1984) Nucleolus organizer polymorphism in barley, Hordeum vulgare L. Hereditas 100:33–43

    Google Scholar 

  • Linde-Laursen I (1988) Giemsa C-banding of barley chromosomes. V. Localization of breakpoints in 70 reciprocal translocations. Hereditas 108:65–76

    Google Scholar 

  • Linde-Laursen I, Bothmer R von (1986) Preferential loss and gain of specific Hordeum vulgare chromosomes in hybrids with three alien species? In: Horn W, Jensen CJ, Odenbach W, Schieder O (eds) Genetic manipulation in plant breeding. de Gruyter, Berlin, pp 179–182

    Google Scholar 

  • Linde-Laursen I, Bothmer R von (1988) Elimination and duplication of particular Hordeum vulgare chromosomes in aneuploid interspecific Hordeum hybrids. Theor Appl Genet 76:897–908

    Google Scholar 

  • Linde-Laursen I, Bothmer R von, Jacobsen N (1980) Giemsa C-banding in Asiatic taxa from Hordeum section Stenostachys with notes on chromosome morphology. Hereditas 93:235–254

    Google Scholar 

  • Linde-Laursen I, Bothmer R von, Jacobsen N (1985) Giemsa C-banded karyotypes of Hordeum taxa from North America. Can J Genet Cytol 28:42–62

    Google Scholar 

  • Mohanty BD, Ghosh PD, Maity S (1986) Chromosome behaviour in long term callus culture in Hordeum vulgare L. Chromosome Inf Serv 41:10–11

    Google Scholar 

  • Orton TI (1980) Haploid barley regenerated from callus cultures of Hordeum vulgare × H. jubatum. J. Hered 71:280–282

    Google Scholar 

  • Rieger R, Michaelis A, Schubert I, Kaina B (1977) Effects of chromosome repatterning in Vicia faba L. II. Aberration clustering after treatment with chemical mutagens and X-rays as affected by segment transposition. Biol Zentralbl 96:161–182

    Google Scholar 

  • Sacristan MD (1971) Karyotypic changes in callus cultures from haploid and diploid plants of Crepis capillaris (L) Wallr. Chromosoma 33:273–283

    Google Scholar 

  • Scheunert EU, Shamina ZB, Koblitz H (1978) Karyological features of barley callus tissues cultured in vitro. Biol Plant 20:305–308

    Google Scholar 

  • Singh RJ (1986) Chromosomal variation in immature embryo derived calluses of barley (Hordeum vulgare L.). Theor Appl Genet 72:710–716

    Google Scholar 

  • Vasil IK (1986) Developing cell and tissue culture systems for the improvement of cereal and grass crops. J Plant Physiol 128:193–218

    Google Scholar 

  • Wenzel G, Foroughi-Wehr B (1984) Anther culture of cereals and grasses. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants. Academic Press, New York, pp 311–324

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by K. Tsunewaki

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jørgensen, R.B., Andersen, B. Karyotype analysis of regenerated plants from callus cultures of interspecific hybrids of cultivated barley (Hordeum vulgare L.). Theoret. Appl. Genetics 77, 343–351 (1989). https://doi.org/10.1007/BF00305826

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00305826

Key words

Navigation