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Histochemical studies on experimental heterotopic calcification

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Zusammenfassung

  1. 1.

    Histochemische Veränderungen zellulärer und extrazellulärer Gewebekomponenten während der Entwicklung heterotoper Verkalkungsherde bei Ratten werden beschrieben. Die Verkalkungen wurden durch DHT-Fütterung und lokale mechanische Traumatisierung nach Selye (1962) erzeugt. Nicht DHT-sensibilisierte Tiere dienten als Kontrollen. In beiden Tiergruppen verläuft die erste Gewebsreaktion ähnlich: Ödembildung, starke Synthesetätigkeit der Bindegewebszellen, Abgabe der Mastzellgranula, starke Zunahme der Aktivität alkalischer Phosphatase. Bei DHT-sensibilisierten Ratten erscheinen später feine Mineralniederschläge, die sich zu großen Verkalkungsherden entwickeln.

  2. 2.

    Ratten mit in Entwicklung begriffenen Kalkherden erhielten eine homologe, Fluorochrom-markierte Serumproteinfraktion injiziert, die aus Albumin, α-1-Globulin, Transferrin und Haptoglobin bestand. Fluoreszenzmikroskopische Untersuchung ergibt, daß dieses Protein, ausschließlich während der aktiven Mineralisierung, selektiv von der verkalkenden Bindegewebsgrundsubstanz aufgenommen und gebunden wird.

  3. 3.

    Dieser Befund legt zusammen mit den Beobachtungen von Lipp (1966, 1967) an Knochen und unseren vorläufigen Ergebnissen an Coronararterien und Nieren die Vermutung nahe, daß Serumproteine als Calciumträger wirken, die spezifisch mit der verkalkungsfähigen Grundsubstanz reagieren.

Summary

  1. 1.

    The histochemical changes of cellular and extracellular components during the development of experimental heterotopic calcifications (provoked in rats by dihydrotachysterol-(DHT)-feeding and local mechanical injury after Selye, 1962) were investigated. Non-DHT-sensitized animals with skin injuries served as controls. In both groups the first reactions were similar. An edema and heavy synthetic activities of connective tissue cells developed. The discharge of mast cell granules, and increased alkaline phosphatase activity were also observed. Only in DHT-sensitized animals fine mineral deposits appeared and developed into large calcifying plaques.

  2. 2.

    Rats with developing heterotopic calcifications were also injected with a fluorochrome labeled homologous serum protein fraction containing albumin, α1-globulin, transferrin, and haptoglobin. Fluorescence microscopy revealed the specific and selective uptake and binding of this protein by the connective tissue matrix simultaneously with the inorganic calcium deposition.

  3. 3.

    These findings suggest together with the results reported by Lipp (1966, 1967) on bones and our preliminary observations on coronary arteries and kidneys that serum protein may function as a calcium carrier which reacts specifically with the calcifiable ground substance.

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Authors and Affiliations

  1. Department of Anatomy, Wayne State University, School of Medicine, Detroit, Michigan, USA

    Helmut H. Wolff

  2. Anatomisches Institut der Universität, Koellikerstr 6, 87, Würzburg

    Helmut H. Wolff

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  1. Helmut H. Wolff
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Additional information

Supported by United States Public Health Service Grant No. AM 06705.

I would like to thank Professor Dr. med. W. W. Lipp for providing his facilities and his help and advice during the experiments and the preparation of the manuscript.

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Wolff, H.H. Histochemical studies on experimental heterotopic calcification. Histochemie 9, 354–366 (1967). https://doi.org/10.1007/BF00305818

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  • DOI: https://doi.org/10.1007/BF00305818

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