Skip to main content
SpringerLink
Log in

Blood serum proteins and the mineralization of bone ground substance

  • Published:
Histochemie Aims and scope Submit manuscript

Zusammenfassung

Gewisse Anteile von Fluorochrom-markiertem homologem Blutserum lassen sich nach parenteraler Verabreichung in der Knochensubstanz junger und erwachsener Ratten direkt nachweisen. Diese Serumproteine werden in hoher Konzentration während der Knochenbildung in die organische Matrix eingebaut. Sie behalten ihre Markierungsfluoreszenz für lange Zeit und widerstehen der Fixierung und der Entkalkung. Während experimenteller Rachitis ist eine Aufnahme dieser markierten Serumproteine in das unverkalkte rachitische Osteoid nicht festzustellen. Ihre Inkorporation beginnt jedoch wieder gleichzeitig mit dem Einsetzen der Verkalkung, wenn die Rachitis zur Ausheilung gebracht wird. Die experimentellen Resultate deuten darauf hin, daß ein calciumtragendes Serumprotein mit gewissen Produkten der Osteoblasten Komplexe bildet, dabei präzipitiert und zu einem Bestandteil der Appositionssäume wird, der möglicher Weise die Verkalkung der organischen Knochengrundsubstanz in Gang setzt. Fraktionierungsexperimente zur Bestimmung der an diesem Vorgang beteiligten Serumkomponente(n) sind noch nicht abgeschlossen. Sie halten derzeit bei einer Fraktion, die Albumin, α-1-Makroglobuline, Transferrin und Haptoglobin enthält, und werden weiter fortgesetzt.

Summary

Direct tracing experiments with fluorochrome-labeled homologous blood serum show that certain serum components are taken up by the bone substance of young and adult rats. These proteins are concentrated and incorporated into the organic matrix while it is being formed. They retain their fluorescent label for long periods of time and withstand histological fixation and decalcification. In experimental rickets, no labeled serum protein is seen to be incorporated into the uncalcified rachitic osteoid. Its uptake begins, however, concomitantly with the onset of mineralization during the healing period. The results of experiments are interpreted in terms of a calcium-carrying serum protein being complexed and precipitated by osteoblast products to form an essential component of apposition zones which, possibly, initiates nucleation and the subsequent steps of calcification. Fractionation experiments to define the serum component(s) involved in this process, will be continued. So far, they have resulted in a fraction containing albumin, α 1-macroglobulin, transferrin, and haptoglobin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Beneke, G.: Modelluntersuchungen zur Reaktion zwischen Mucopolysacchariden und Plasmaeiweißkörpern. Verh. dtsch. Ges. Path. 48, 306–309 (1964).

    Google Scholar 

  • Bronner, F.: Dynamics and function of calcium. In: Mineral metabolism (eds. C. L. Comar and F. Bronner), vol. II, part A, p. 341–444. New York and London: Academic Press 1964.

    Google Scholar 

  • Chadwick, C. S., and J. E. Fothergill: Fluorochromes and their conjugation with proteins. In: Fluorescent protein-tracing (ed. B. C. Nairn), p. 4–30. Edingburgh and London: Livingstone 1962.

    Google Scholar 

  • Eger, W.: Über die Mineralisation des Knochengewebes unter Berücksichtigung autoradiographischer Befunde. Acta histochem. (Jena), Suppl. 3, 16–30 (1963).

    Google Scholar 

  • Fleisch, H.: Role of nucleation and inhibition in calcification. Clin. Orthop. 32, 170–180 (1964).

    Google Scholar 

  • Frost, H. M.: Preparation of thin undecalcified bone sections by rapid manual method. Stain Technol. 33, 273–277 (1958).

    Google Scholar 

  • Glimcher, M. J.: Specificity of the molecular structure of organic matrices in mineralization. In: Calcification in biological systems (ed. R. F. Sognnaes), p. 421–487. Washington, D. C., Amer. Assoc. Advanc. Sci., Publ. No 64 (1960).

    Google Scholar 

  • —, and S. M. Krane: Studies of the interactions of collagen and phosphate. I. The nature of inorganic phosphate binding. In: Radioisotopes and bone (eds. F. McLean, P. Lacroix and A. M. Budy), p. 393–418. Oxford (Engl.): Blackwell 1962.

    Google Scholar 

  • Hirschman, A., and D. D. Dziewiatkowski: Protein-polysaccharide loss during endochondial ossification: immunochemical evidence. Science 154, 393–395 (1966).

    Google Scholar 

  • Irving, J. T.: Dynamics and function of phosphorus. In: Mineral metabolism (eds. C. L. Comar and F. Bronner), vol. II, part A, p. 249–313. New York and London: Academic Press 1964.

    Google Scholar 

  • Knese, K. -H.: Über die Mineralablagerungen im Knorpel- und Knochengewebe unter Berücksichtigung elektronenmikroskopischer Befunde. Acta histochem. (Jena), Suppl. 3, 31–56 (1963).

    Google Scholar 

  • —: Zytogenese und topochemische Reaktion der frühen und späten epitheloiden Osteoblasten. Z. Zellforsch. 69, 93–128 (1966).

    Google Scholar 

  • Lipp, W.: Neuuntersuchungen des Knochengewebes. I. Die Knochenzellen und ihre Grenzscheiden. Acta anat. (Basel) 20, 162–200 (1954). — II. Histologisch erfaßbare Lebensäußerungen der Knochenzellen. Acta anat. (Basel) 22, 151–201 (1954). — III. Histologisch erfaßbare Lebensäußerungen der Osteozyten in embryonalen Knochen des Menschen. Anat. Anz. 102, 361–372 (1956).

    Google Scholar 

  • —: Aminopeptidase in bone cells. J. Histochem. Cytochem. 7, 205 (1959).

    Google Scholar 

  • —: Blood serum proteins and the formation of bone ground substance. Anat. Rec. 154, 471–472 (1966).

    Google Scholar 

  • Mancini, R. E., O. Vilar, J. M. Dellacha, O. W. Davidson, C. J. Gomez, and B. Alvarez: Extravascular distribution of fluorescent albumin, globulin and fibrinogen in connective tissue structures. J. Histochem. Cytochem. 10, 194–203 (1962).

    Google Scholar 

  • —, C. Gomez, J. M. Dellacha, O. W. Davidson, and A. Castro: Histological study of distribution of fluorescent serum proteins in connective tissue. J. Histochem. Cytochem. 9, 356–362 (1961).

    Google Scholar 

  • —, J. J. Heinrich, O. W. Davidson, and B. Alvarez: Transference of circulating labeled serum proteins to the follicle of the rat ovary. J. Histochem. Cytochem. 11, 80–88 (1963).

    Google Scholar 

  • Manunta, G., J. Saroff, and C. W. Turner: Paper electrophoretic study of Ca45 binding in sera of normal, parathyroidectomized and estrogen treated rats. Proc. Soc. exp. Biol. (N.Y.) 94, 790–791 (1957).

    Google Scholar 

  • Mayersbach, H.: Zur Frage des Proteinüberganges von der Mutter zum Foeten. I. Befunde an Ratten am Ende der Schwangerschaft. Z. Zellforsch. 48, 479–504 (1958a).

    Google Scholar 

  • —: Immunohistologische Methoden in der Histochemie. In: Handbuch der Histochemie (Hrsg. W. Graumann und K. Neumann), Bd. I/1, S. 599–639. Stuttgart: Gustav Fischer 1958b.

    Google Scholar 

  • —: Immunohistologische Methoden in der Histochemie; Ergänzungsbeitrag zu Bd. I/1. In: Handbuch der Histochemie (Hrsg. W. Graumann und K. Neumann), Bd. I/2, S. 188–268. Stuttgart: Gustav Fischer 1966.

    Google Scholar 

  • Mc Lean, F. C., and M. R. Urist: Bone. An introduction to the physiology of skeletal tissue, 2nd ed. Chicago: Univ. of Chicago Press 1961.

    Google Scholar 

  • Nairn, R. C.: Fluorescent protein tracing. Edinburgh and London: Livingstone. 1st ed. (1962), 2nd ed. (1964).

    Google Scholar 

  • Neuman, W. F., and M. W. Neuman: The chemical dynamics of bone mineral. Chicago: Univ. of Chicago Press 1958.

    Google Scholar 

  • Ratzenhofer, M., u. W. Maresch: Über das Vorkommen von Hämagglutininen in der Gewebsflüssigkeit. Virchows Arch. path. Anat. 331, 510–517 (1958).

    Google Scholar 

  • Sheldon, H., and R. A. Robinson: Studies on rickets. I. The fine structure of uncalcified bone matrix in experimental rickets. Z. Zellforsch. 53, 671–684 (1961). - II. The fine structure of the cellular components of bone in experimental rickets. Z. Zellforsch. 53, 685–701 (1961).

    Google Scholar 

  • Sobel, A. E., M. Burger, and S. Nobel: Mechanisms of nuclei formation in mineralizing tissue. Clin. Orthop. 17, 103–123 (1960).

    Google Scholar 

  • —, P. A. Lawrence, and S. Nobel: Nuclei formation and crystal growth in mineralizing tissues. J. Indiana dent. Ass. 40, 47–58 (1961).

    Google Scholar 

  • Termine, J. D., and A. S. Posner: Infrared analysis of rat bone: Age dependancy of amorphous and crystalline mineral fractions. Science 153, 1523–1525 (1966).

    Google Scholar 

  • Urist, M. R.: Recent advances in physiology of calcification. J. Bone Jt Surg. A 46, 889–900 (1964).

    Google Scholar 

  • —, and F. C. Mc Lean: The partition and binding of calcium in the serum of the laying hen and of the estrogenized rooster. Endocrinology 63, 570–585 (1958).

    Google Scholar 

  • —, and A. O. Schjeide: The partition of calcium and protein in the blood of oviparous vertebrates during estrus. J. gen. Physiol. 44, 743–756 (1961).

    Google Scholar 

  • Wasserman, R. H., and A. N. Taylor: Vitamin D3-induced calcium-binding protein in chick intestinal mucosa. Science 152, 791–793 (1966).

    Google Scholar 

  • Wolff, H. H.: Histochemical studies on experimental heterotopic calcification. Histochemie 9, 354–366 (1967).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, School of Medicine, Wayne State University, Detroit, Mich.

    Walther Lipp

Authors
  1. Walther Lipp
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by United States Public Health Service Grant No. AM06705.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lipp, W. Blood serum proteins and the mineralization of bone ground substance. Histochemie 9, 339–353 (1967). https://doi.org/10.1007/BF00305817

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00305817

Keywords

Search

Navigation