Summary
Since in histochemical reactions the liver alkaline phosphatase discloses only low activity with respect to beta-glycerophosphate, the authors have replaced the latter by cytidine monophosphate. With this substrate they obtained much better results: greater sensibility permitting to reduce the incubation time, and much more precise localization of the enzyme activity, at the ultrastructural level. Details of the method are given with a discussion of its specifity. The method was applied on liver tissue of normal albino rats and animals of the same strain with ligated common bile duct.
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This study was supported by a grant from the Fonds voor Wetenschappelijk Geneeskundig Onderzoek.
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Oledzka-Slotwińska, H., Creemers, J. & Desmet, V. Cytidine monophosphate as substrate for the electron microscopic visualization of alkaline phosphatase activity. Histochemie 9, 320–326 (1967). https://doi.org/10.1007/BF00305815
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DOI: https://doi.org/10.1007/BF00305815