Summary
Available glutaraldehydes were fractionated on Sephadex G-10 in the pH range 3.0–8.2. Two fractions with absorptions at 235 and 280 mμ were separated, the latter alone having the aldehyde function. The relative elution volume of the aldehyde fraction was pH sensitive. Increase in temperature or pH increased the absorption at 280 mμ, the latter associated with a decrease in the aldehyde content. Glutaraldehyde solutions left three weeks at room temperature and pH 7.2 gave satisfactory fixation of rat liver.
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Hopwood, D. The behaviour of various glutaraldehydes on Sephadex G-10 and some implications for fixation. Histochemie 11, 289–295 (1967). https://doi.org/10.1007/BF00305805
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DOI: https://doi.org/10.1007/BF00305805