Summary
In intact tissue sections, neotetrazolium formazan production is greater when the mitochondrial succinate dehydrogenase and the cytoplasmic glucose-6-phosphate dehydrogenase activities are tested together than when measured individually. Studies with malonate have shown that this increase is due to an increase in the rate of oxidation of succinate. Such an increase does not occur however when the hydrogen transport pathway is by-passed with PMS. It is suggested that the cytoplasmic activity overcomes a rate-limiting step in the mitochondrial transport chain.
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Butcher, R.G. Succinate oxidation in intact tissue sections: the effect of oxidation in the cytoplasm. Histochemie 32, 369–378 (1972). https://doi.org/10.1007/BF00305771
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DOI: https://doi.org/10.1007/BF00305771