Summary
The distribution of 5′-nucleotidase activity in rat liver shows a sexual dependence. In male liver the activity in the bile canalicular wall is most pronounced, whereas the activity at the sinusoidal border of the liver parenchymal cell is slightly more in the female rat. Castration and treatment with sex hormones change the distribution pattern. The greatest variations in enzyme activity are seen at the bile canalicular site of the liver cell. These changes are probably an expression of the altered functional state of the liver cell.
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Christiansen, K., Gan, M. V., Holman, R. T.: Sex differences in the metabolism of fatty acids in vitro. Biochim. biophys. Acta (Amst.) 187, 19–25 (1969).
Deimling, O. v.: Enzymarchitektur der Niere und Sexualhormone. Progr. Histochem. Cytochem. 1, 1–50 (1970).
Duve, C. de: Fissue fractionation, past and present. J. Cell Biol. 50, 20D-55D (1971).
Emmelot, P., Bos, C. J.: Differential effect of neuraminidase on the magnesium ATP-ase, sodium—potassium—magnesium ATP-ase and 5′nucleotidase of isolated plasma membranes. Biochim. biophys. Acta (Amst.) 99, 578–580 (1965).
Emmelot, P., Bos, C. J.: Studies on plasma membranes. V. On the lipid dependence of some phosphohydrolases of isolated rat liver plasma membranes. Biochim. biophys. Acta (Amst.) 150, 341–353 (1968).
Emmelot, P., Bos, C. J.: Studies on plasma membranes. XV. A sex difference in alkaline phosphatase activities of plasma membranes isolated from rat liver. Biochim. biophys. Acta (Amst.) 249, 293–300 (1971).
Erecińska, M., Sierakowska, H., Shugar, D.: Intracellular localization of phosphodiesterases I and II in rat liver. Europ. J. Biochem. 11, 465–471 (1969).
Essner, E., Novikoff, A. B., Masek, B.: ATP-ase and 5′-nucleotidase in the plasma membrane of liver cells as revealed by electron microscopy. J. biophys. biochem. Cytol. 4, 711–716 (1958).
Goldfischer, S., Essner, E., Novikoff, A. B.: The localization of phosphatase activities at the level of ultrastructure. J. Histochem. Cytochem. 12, 72–95 (1964).
Hardonk, M. J.: 5′-Nucleotidase. I. Distribution of 5′-nucleotidase in tissues of rat and mouse. Histochemie 12, 1–17 (1968a).
Hardonk, M. J., Boer, H. G. A. de: 5′-Nucleotidase. III. Determinations of 5′-nucleotidase iso-enzymes in tissues of rat and mouse. Histochemie 12, 29–41 (1968b).
Hardonk, M. J., Hoedemaeker, Ph. J.: Lokalisation der 5-Nukleotidase-Isoenzyme in der Rattenleber. Acta histochem. (Jena), Supplementband X, 385–389 (1971).
Holtzman, J. L., Gram, T. E., Gillette, J. R.: The kinetics of 32P incorporation into the phospholipids of hepatic rough and smooth microsomal membranes of male and female rats. Arch. Biochem. 138, 199–207 (1970).
Jap, P. H. K.: Ultrastructural and histochemical investigations on auxiliary liver grafts in rats, with some notes on the application to other species. Thesis, University of Nijmegen, The Netherlands 1971.
Kato, R., Takanaki, A.: Metabolism of drug in old rats (1). Activities of NADPH-linked electrontransport and drugmetabolizing enzyme systems in liver microsomes of old rats. Jap. J. Pharmacol. 18, 381–388 (1968).
Klevay, L. M.: Dieldrin excretion by the isolated perfused rat liver: a sexual difference. Toxicol. appl. Pharmacol. 17, 813–815 (1970).
Koudstaal, J., Hardonk, M. J.: Histochemical demonstration of enzymes in rat liver during post-natal development. Enzymes related to NADPH dependent hydroxylating systems and to sexdifference. Histochemie 23, 71–81 (1970).
Putzke, H. P., Ernst, B.: Chemisch-analytische und elektronenmikroskopische Untersuchungen zur 5′-Nukleotidase-Aktivität im exokrinen Parenchym und Sekret des Rattenpankreas. Acta biol. med. germ. 26, 665–670 (1971).
Song, C. S., Bodansky, O.: Subcellular localization and properties of 5′-nucleotidase in the rat liver. J. biol. Chem. 242, 694–699 (1967).
Thines-Sempoux, D., Amar-Costesec, A., Beaufay, H., Berthet, J.: The association of cholesterol, 5′-nucleotidase and alkaline phosphodiesterase I with a distinct group of microsomal particles. J. Cell Biol. 43, 189–192 (1969).
Touster, O., Aronson, N. N., Dulaney, J. T., Hendrickson, H.: Isolation of rat liver plasma membranes. Use of nucleotide pyrophosphatase and phosphodiesterase I as marker enzymes. J. Cell Biol. 47, 604–618 (1970).
Wattiaux-de Coninck, S., Wattiaux, R.: Nucleoside diphosphatase activity in plasma membrane of rat liver. Biochim. biophys. Acta (Amst.) 183, 118–128 (1969).
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Hardonk, M.J., Jonkers, A. 5′-nucleotidase. Histochemie 32, 363–368 (1972). https://doi.org/10.1007/BF00305770
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DOI: https://doi.org/10.1007/BF00305770