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Measurements of the affinities of basic and “mordant” dyes for various tissue substrates

Histochemie volume 36, pages 303–312 (1973)Cite this article

Summary

A quantitative measure of the affinity of a dye for a substrate is given by: Affinity =

$$- \Delta G^o {\text{ = }}RT ln \frac{1}{{C_d }}$$

(where R is the gas constant, T the absolute temperature, C d the concentration of the dyebath). Affinities have been measured for several tissue substrates with a range of basis and “mordant” dyes.

The results obtained have been used to study the mechanism of several staining procedures of special interest. For example, the Critical Electrolyte Concentration effects could not, contrary to previous suggestions, be correlated with differential dye-substrate affinities in the salt-free situation. Another application was the study of methyl green-pyronin type stains for differential staining of DNA and RNA. The results failed to support the theories requiring specific dye-nucleic acid affinities. Finally an attempt was made to investigate the mode of action of “mordant” dyes of the preformed metal complex type. The results suggested that some dyes of these at least do not bind via the metal ion.

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References

  • Baker, J. R.: Principles of biological microtechnique. London: Methuen 1970

    Google Scholar 

  • Baker, J. R., Williams, E. G. M.: The use of methyl green as a histochemical reagent. Quart. J. micr. Sci. 106, 3–13 (1965)

    Google Scholar 

  • Berube, G. R., Powers, M. M., Clark, G.: The cationic chelate of chromium and aluminon as a selective nuclear stain. Stain Technol. 39, 337–338 (1964)

    Google Scholar 

  • Berube, G. R., Powers, M. M., Kerkay, T., Clark, G.: The gallocyanin-chrome alum stain; influence of methods of preparation on its activity and separation of active staining compound. Stain Technol. 41, 73–81 (1966)

    Google Scholar 

  • Culling, C. F. A.: Handbook of histopathological techniques. London: Butterworths 1963

    Google Scholar 

  • Einarson, L.: A method for progressive staining of Nissl and nuclear substances in nerve cells. Amer. J. Path. 8, 295–305 (1932)

    Google Scholar 

  • Friedberg, S. H., Goldstein, D. J.: Thermodynamics of orcein staining of elastic fibres. Histochem. J. 1, 361–376 (1969)

    Google Scholar 

  • Goldstein, D. J.: Mechanism of differential staining of nucleic acids. Nature (Lond.) 191, 407–408 (1961)

    Google Scholar 

  • Goldstein, D. J.: An approach to the thermodynamics of histological dyeing, illustrated by experiments with azur A. Quart. J. micr. Sci. 104, 413–439 (1963)

    Google Scholar 

  • Gurr, E.: Encyclopaedia of microscopic stains. London: Leonard Hill 1960

    Google Scholar 

  • Kurnick, N. B.: Histochemistry of nucleic acids. Int. Rev. Cytol. 4, 221–268 (1955)

    Google Scholar 

  • Lillie, R. D.: H. J. Conn's biological stains, 8th ed. Baltimore: Williams & Wilkins 1969

    Google Scholar 

  • Long, C.: (Ed.) The Biochemist's Handbook, 2nd ed. London: Spon 1961

    Google Scholar 

  • Marshall, P. N., Horobin, R. W.: The chemical nature of the gallocyaninchrome alum staining complex. Stain Technol. 47, 155–161 (1972)

    Google Scholar 

  • Pappenheim, A.: Vergleichende Untersuchungen über die elementare Zusammensetzung des roten Knochenmarks einiger Säugetiere. Virchows Arch. path. Anat. 157, 19–76 (1899)

    Google Scholar 

  • Sandritter, W., Kiefer, G., Rick, W.: Gallocyanin-chrome alum. In: Introduction to quantitative cytochemistry (ed. G. L. Wied). New York: Academic Press 1966

    Google Scholar 

  • Scott, J. E.: On the mechanism of the methyl green-pyronin stain for nucleic acids. Histochemie 9, 30–47 (1967)

    Google Scholar 

  • Scott, J. E.: Patterns of specificity in the interaction of organic cations with acid mucopoly-saccharides. In: The chemical physiology of mucopolysaccharides (ed. G. Quintarelli). London: Churchill 1968

    Google Scholar 

  • Scott, J. E., Willett, I. H.: Binding of cationic dyes to nucleic acids and other biological polyanions. Nature (Lond.) 209, 985–987 (1966)

    Google Scholar 

  • Stockwell, R. A., Scott, J. E.: Observations on the acid glycosaminoglycan (mucopoly-saccharide) content of the matrix of ageing cartilage. Ann. rheum. Dis. 24, 341–350 (1965)

    Google Scholar 

  • Vickerstaff, T.: The physical chemistry of dyeing. London: Oliver & Boyd 1954

    Google Scholar 

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Author notes

  1. P. N. Marshall

    Present address: Department of Haematology, Royal Postgraduate Medical School, Hammersmith Hospital, W. 12, London

Authors and Affiliations

  1. Department of Human Biology and Anatomy, University of Sheffield, Sheffield, UK

    P. N. Marshall & R. W. Horobin

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  1. P. N. Marshall
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  2. R. W. Horobin
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Marshall, P.N., Horobin, R.W. Measurements of the affinities of basic and “mordant” dyes for various tissue substrates. Histochemie 36, 303–312 (1973). https://doi.org/10.1007/BF00305709

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  • DOI: https://doi.org/10.1007/BF00305709

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