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Spontaneous variations in the cell population of a T-cell lymphoma during tissue culture and transplantation

Spontanvariationen in der Zellpopulation eines T-Zell Lymphoms in Zellkultur und nach Isotransplantation

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Summary

Immunologically induced T-cell lymphomas were kept for long periods of time in isotransplantation and in tissue culture. Initially, a certain number of cells within the tumor showed differentiation towards histiocytes. These disappeared completely after about the twentieth graft generation when the tumor changed toward a more atypical and more uniform neoplasm. That change was correlated with a decrease in transplantability, a limited growth in tissue culture, and a decrease in density of theta antigens on tumor cell membranes. While the original tumor and early grafts contained occasional C-type particles, these were never demonstrated in late tumor grafts. The possible implications of these changes are discussed.

Zusammenfassung

Ein immunologisch induziertes T-Zell Lymphom der Maus wurde über längere Zeit in der Zellkultur und in Isotransplantation gehalten. Zu Beginn war eine gewisse Differenzierungspotenz einzelner Zellen im Tumor in Richtung auf Histiocyten erkennbar, die später zugunsten einer mehr homogenen atypischen Lymphoblastenpopulation verloren ging. Diese Änderung wurde besonders in transplantierten Tumoren nach der 20. Generation erkennbar. Gleichzeitig kam es zu einer Zunahme bestimmter Cytoplasmastrukturen und zu einer Abnahme der Theta-Antigen Häufigkeit sowie zu einer herabgesetzten Angehrate des Tumors bei Transplantation und zu begrenztem Wachstum in der Zellkultur. Anfangs beobachtete C-Typ Partikel ließen sich später nicht mehr nachweisen. Die mögliche Bedeutung dieser Befunde wird diskutiert.

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Authors and Affiliations

  1. Laboratories of Immunopathology and Section of Ultrastructural Research, Pathology Institute, University of Cologne, Cologne, Germany

    G. R. F. Krueger, H. -E. Schaefer, J. Luetzeler, R. Fischer & V. Kresin

Authors
  1. G. R. F. Krueger
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  2. H. -E. Schaefer
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  3. J. Luetzeler
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  4. R. Fischer
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  5. V. Kresin
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Additional information

The investigations are supported by the German Science Foundation and by the Volkswagen Foundation.

We are indebded to Dr. Gregory T. O'Conor, National Cancer Institute, NIH, U.S.A., for assistence in some of the ultrastructural work.

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Krueger, G.R.F., Schaefer, H.E., Luetzeler, J. et al. Spontaneous variations in the cell population of a T-cell lymphoma during tissue culture and transplantation. Z. Krebsforsch. 84, 25–35 (1975). https://doi.org/10.1007/BF00305686

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  • DOI: https://doi.org/10.1007/BF00305686

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