Summary
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1.
The use of a new grade of polyvinyl alcohol (PVA) is described for maintaining tissue integrity during histochemical incubations. The new material has several advantages over the PVA currently used. It is somewhat less viscous and easier to prepare, and it has no reducing effect upon tetrazolium salts.
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2.
No losses of nitrogenous material, or of the “soluble” enzyme 6 phosphogluconate dehydrogenase, could be detected from unfixed 10 micron sections of rat liver after incubation in media containing 22 % w/v of the new PVA.
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Altman, F.P. The use of a new grade of polyvinyl alcohol for stabilising tissue sections during histochemical incubations. Histochemie 28, 236–242 (1971). https://doi.org/10.1007/BF00305680
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DOI: https://doi.org/10.1007/BF00305680