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Studies in fluorescence histochemistry

X. Optimum conditions of the acetic anhydride-salicylhydrazide-zinc (or fluorescent ketone) technique for demonstrating C-terminal carboxyl groups of proteins

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Summary

The optimum conditions for eliciting the strongest fluorescence from proteins in skeletal and smooth muscle, stratified squamous epithelium and hyaline cartilage with the acetic anhydride-salicylhydrazide-zinc method for localizing C-terminal carboxyl groups were determined quantitatively with a microscope microfluorimeter and found to be those stated in the original method of Stoward and Burns (1967).

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References

  • Barrnett, R. J., Seligman, A. M.: Histochemical demonstration of protein bound acylamide carboxyl groups. J. biophys. biochem. Cytol. 4, 169–174 (1958).

    Google Scholar 

  • Burns, J., Stoward, P. J.: Studies in fluorescence histochemistry, VIII. Evidence that N-acetyl groups do not take part in the acetic anhydride-salicylhydrazide-zinc (or fluorescent ketone) method for demonstrating protein C-terminal carboxyl groups. Histochemie 26, 266–271 (1971a).

    Google Scholar 

  • —: Studies in fluorescence histochemistry, IX. Evidence that S-acetyl and O-acetyl groups do not take part in the acetic anhydride-salicylhydrazide-zinc (or fluorescent ketone) technique for localizing protein C-terminal carboxyl groups. Histochemie 26, 272–278 (1971b).

    Google Scholar 

  • Stoward, P. J.: Studies in fluorescence histochemistry, III. The demonstration with salicyl-hydrazide of the aldehydes present in periodate-oxidized mucosubstances. J. roy. micr. Soc. 87, 247–257 (1967).

    Google Scholar 

  • —: A simple microfluorimeter. Proc. roy. micr. Soc. 3, 122–125 (1968a).

    Google Scholar 

  • —: Studies in fluorescence histochemistry, V. The influence of trace metals on the fluorescence of periodate-oxidized mucosubstance salicylhydrazones. J. roy. micr. Soc. 88, 571–585 (1968b).

    Google Scholar 

  • Stoward, P. J.: Burns, J.: Studies in fluorescence histochemistry, IV. The demonstration of the C-terminal carboxyl groups of proteins. Histochemie 10, 230–233 (1967).

    Google Scholar 

  • —: Some evidence confirming the specificity of Barrnett and Seligman's technique for demonstrating side-chain carboxyl groups in tissue proteins. Histochemie 11, 7–10 (1968).

    Google Scholar 

  • —: Studies in fluorescence histochemistry, VII. The mechanism of the complex reactions that may take place between protein carboxyl groups and hot mixtures of acetic anhydride and pyridine in the acetic anhydride-salicylhydrazide-zinc (or fluorescent ketone) method for localizing protein C-terminal carboxyl groups. Histochemie J. 3, 127–141 (1971).

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Gibson Laboratories, Radcliffe Infirmary, Oxford

    J. Burns & P. J. Stoward

  2. The Department of Anatomy, University of Dundee, Scotland

    J. Burns & P. J. Stoward

Authors
  1. J. Burns
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  2. P. J. Stoward
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Burns, J., Stoward, P.J. Studies in fluorescence histochemistry. Histochemie 26, 279–288 (1971). https://doi.org/10.1007/BF00305661

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  • DOI: https://doi.org/10.1007/BF00305661

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