Summary
The presence of polysaccharides connected with microtubules in the lipotubuloids of Ornithogalum umbellatum L. was detected by the silver hexamine method and Ruthenium Red staining. Polysaccharides are localized on the outer surface of microtubules. Staining of polysaccharides by Ruthenium Red causes an increase in the microtubule walls thickness by 2.5 nm and in their diameter by 4.5 nm.
Similar content being viewed by others
References
Flaxa, M. L., Gill, T. J.: III-Preparation and properties of an alkylated brain protein related to the structural subunit of microtubules. Arch. Biochem. Biophys. 135, 194 (1967)
Kwiatkowska, M.: Ultrastrukturalne, cytochemiczne i przyżyciowe badania elajoplastów roślin kwiatowych, Łódź, University of Łódź 1970
Kwiatkowska, M.: Changes in the diameter of microtubules connected with the autonomous rotary motion of the lipotubuloids (elaioplasts). Protoplasma (Wien) 75, 345–357 (1972)
Kwiatkowska, M.: Half unit membranes surrounding osmiophilic granules (lipid droplets) of so-called lipotubuloid in Ornithogalum umbellatum. Protoplasma (Wien) 77, 473–476 (1973)
Lane, N. J., Treherne, J. E.: Lanthanium staining of neurotubules in axons from cockroach ganglia. J. Cell Sci. 7, 217–231 (1970)
Luft, J. H.: Ruthenium red staining of the striated muscle cell membrane and the myotendinal junction. In Proc. 6th Int. Cong. for Electron Microscopy vol. 2 Tokyo, 65–66 (1966)
Pickett-Heaps, J. D.: Further ultrastructural observations on polysaccharide localization in plant cells. J. Cell Sci. 3, 55–64 (1968)
Stephens, R. E.: The mitotic apparatus. Physiological, chemical characterisation of the 22 S protein component and its subunits. J. Cell Biol. 32, 255 (1967)
Tani, E., Ametani, T.: Substructure of mierotubules in brain nerve cells as revealed by ruthenium red. J. Cell Biol. 46, 159–165 (1970)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kwiatkowska, M. Polysaccharides connected with microtubules in the lipotubuloids of Ornithogalum umbellatum L.. Histochemie 37, 107–112 (1973). https://doi.org/10.1007/BF00305581
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00305581