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DOPA compared with dihydroxyfumarate as co-factor in peroxidase-mediated oxidation of tyrosine to melanin

Histochemical studies with neutrophils, eosinophils, mast cells, melanoma cells and neurons

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Summary

Dihydroxyfumarate was used as a co-factor in the histochemical demonstration of peroxidase-mediated oxidation of tyrosine to melanin in eosinophils, mast cells, melanoma cells and neurons. The use of dihydroxyfumarate as co-factor provides a direct method for demonstrating peroxidase-mediated oxidation of tyrosine to melanin in tissues, and allows for the ultrastructural localization of this pigment.

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References

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Authors and Affiliations

  1. Departments of Dermatology, Tufts University School of Medicine and Boston City Hospital, Boston, Massachusetts

    Milton R. Okun, Ravindra P. Patel, Barbara Donnellan & Walter F. Lever

  2. Department of Pathology, St. Vincent Hospital and University of Massachusetts School of Medicine, Worcester, Massachusetts

    Leon M. Edelstein & David Epstein

Authors
  1. Milton R. Okun
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  2. Ravindra P. Patel
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  3. Barbara Donnellan
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  4. Walter F. Lever
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  5. Leon M. Edelstein
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  6. David Epstein
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Additional information

Supported by USPHS Grant T1 AM 5220 and by the St. Vincent Hospital Research Foundation.

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Okun, M.R., Patel, R.P., Donnellan, B. et al. DOPA compared with dihydroxyfumarate as co-factor in peroxidase-mediated oxidation of tyrosine to melanin. Histochemie 27, 331–334 (1971). https://doi.org/10.1007/BF00305335

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  • DOI: https://doi.org/10.1007/BF00305335

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